Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   ACK2WG_RS05795 Genome accession   NZ_CP176536
Coordinates   1135308..1139012 (+) Length   1234 a.a.
NCBI ID   WP_409509596.1    Uniprot ID   -
Organism   Bacillus spizizenii strain 29-4     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1130308..1144012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2WG_RS05780 (ACK2WG_05780) - 1130754..1131083 (-) 330 WP_041520913.1 four-helix bundle copper-binding protein -
  ACK2WG_RS05785 (ACK2WG_05785) - 1131200..1131637 (-) 438 WP_014113300.1 ferritin family protein -
  ACK2WG_RS05790 (ACK2WG_05790) addB 1131821..1135321 (+) 3501 WP_409509595.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ACK2WG_RS05795 (ACK2WG_05795) addA 1135308..1139012 (+) 3705 WP_409509596.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ACK2WG_RS05800 (ACK2WG_05800) sbcD 1139084..1140259 (+) 1176 WP_041904943.1 exonuclease subunit SbcD -
  ACK2WG_RS05805 (ACK2WG_05805) sbcC 1140256..1143648 (+) 3393 WP_409509597.1 exonuclease subunit SbcC -
  ACK2WG_RS05810 (ACK2WG_05810) hlpB 1143662..1143964 (+) 303 WP_014113305.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141398.79 Da        Isoelectric Point: 5.1261

>NTDB_id=1080761 ACK2WG_RS05795 WP_409509596.1 1135308..1139012(+) (addA) [Bacillus spizizenii strain 29-4]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPFYQYVKEDIAMVLNGAKEKLQRA
LELTKAPGGPAPRADNFLDDLGQLDELIQHQDDYTELYKRVPAVSFKRAKAVKGDEFDPALLEEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLADMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILTAENETGERQPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDKTETELLLIDNAEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLETFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQLAKWQASASQTDWLLPEFDRYQAKTYLDFIGPALARHRDMEVLAENDAAMHADISGHPARFAVRMIHSYD
LLDDDLEERTEEKSERLEAIRKGEPVPGSFAFDEKAREQLSWTYPHHEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHIPTIEEAEQTVHKLYEKELLTEEQKNAIDIEEIVQFFHTEIGG
QLIGAKWKDREVPFSLALPAKEIYPDAQEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=1080761 ACK2WG_RS05795 WP_409509596.1 1135308..1139012(+) (addA) [Bacillus spizizenii strain 29-4]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTCTCAACCGGCCAGGATATTCT
CGTGGCAGCGGCGGCGGGCTCCGGTAAAACCGCTGTGCTCGTTGAACGAATGATCCGGAAAATCACCGCGGAAGAAAACC
CGATTGATGTAGACCGGCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAACACCGGATCGCGGATGCC
CTGGAAAAAGAGCTTGTAAAGCGCCCCGGCTCGCTGCATATCAGACGCCAGCTGTCTCTTTTAAACCGGGCCAGCATTTC
GACACTTCATTCCTTTTGCCTGCAAGTGCTGAAAAAATATTACTATTTGATCGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTGCTCGGGGATGAGGTGCTGGACGAGCTGTTTGAAGACGAATACGCAAAGGGCGAAAAGGCG
TTTTTTGAACTTGTTGACCGCTACACGACAGACCGGCACGATCTGGATCTGCAATTTCTCGTCAAACAGGTGTACGAGTA
TTCACGTTCCCACCCAAACCCGGAGGCATGGCTGGAAAGCTTTGTTCATTTGTATGATGTATCGGAAAAGAGCGCCATCG
AGGAGCTTCCGTTTTATCAATATGTCAAAGAGGATATTGCGATGGTGCTTAACGGAGCAAAGGAGAAGCTTCAGCGCGCG
CTTGAGCTGACGAAAGCGCCGGGCGGCCCCGCGCCGCGCGCTGACAATTTTCTTGATGATCTTGGGCAGCTTGATGAACT
GATTCAGCATCAGGATGATTACACTGAGCTTTATAAGCGGGTGCCCGCAGTCTCTTTTAAGCGCGCGAAAGCAGTAAAAG
GGGACGAGTTTGATCCTGCACTCCTTGAAGAAGCGACAGATTTGAGAAACGGTGCAAAAAAACTGCTTGAAAAGCTTAAA
ACCGACTACTTCACAAGAAGTCCTGAACAGCATTTGAAAAGCTTGGCCGACATGAAGCCTGTTATTGAAACGCTCGTACA
GCTTGTCATCAGCTATGGAAAACGGTTCGAAGCCGCGAAGCAGGAAAAATCAATCATTGATTTTTCTGATTTGGAGCATT
ACTGTTTATCCATTTTGACAGCTGAGAATGAAACAGGTGAGCGCCAGCCGAGCGAGGCAGCAAGATTTTATCAAGAGCAG
TTTCATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTAGTGCAGGAATCGATCCTGCAGCTCGTCACAAGCGGACC
GGAGGAAACAGGCAACCTGTTTATGGTAGGTGATGTCAAACAGTCCATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TCCTCTCCAAATACAAACGGTTTACAGAGAGCGGAGAAGGCACAGGACGGAAAATTGACTTGAATAAAAATTTCCGAAGC
CGGGCTGATATTTTAGATAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTTGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCGGCGTATCCGGACAATGATAAGACGGAAACGGAGCTGCTGCTGATCGACAACGCAG
AAGAGACGGACGCAGGCGAAGAAGCAGAAGAGCTGGAAACGGTGCAGTTCGAGGCGAAAGCGATCGCTAAGGAAATTCGC
AAGCTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTGCTCCGCTCCATGCCGTGGGCGCCGCAAATCATGGAGGAGCTGAGAGCTCAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGCTATTTTGAAGCGGTCGAAGTTGCCGTCGCGCTTTCTGTGCTGAAGGTAATCGATAACCCGTATCAGGATATA
CCGCTTGCCTCTGTTCTGCGCTCACCAATTGTCGGAGCGGATGAAAACGAGCTCTCTTTGATCCGGCTTGAAAATAAAAA
AGCGCCGTACTATGAAGCGATGAAAGACTATTTGGCTGCCGGTGACCGGAACGACGAGCTTTATCAAAAGCTGGAAACGT
TCTACGGACATCTTCAAAAATGGCGTGCGTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAC
ACGAAATACATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGTGTTCTCTATGACCGTGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTCCGTTTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGAACGGCGCGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGTCTCGAG
TTTCCCGTCGTGTTTGCAGCAGGTCTCGGCCGGAATTTCAACATGATGGACTTGAACAAATCGTACCTGCTGGACAAGGA
GCTTGGGTTCGGCACGAAGTATATTCATCCGCAATTGCGCATCAGCTACCCGACGCTGCCTCTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCAGAGGAATTGCGTGTTCTCTATGTTGCATTAACGAGAGCGAAAGAAAAACTGTTTCTG
ATTGGCTCGTGCAAGGATCATCAGAAACAGCTCGCAAAATGGCAGGCATCCGCGTCTCAAACTGACTGGCTTCTTCCGGA
ATTTGACCGTTATCAGGCAAAAACGTATTTAGATTTCATCGGACCGGCTCTTGCCAGACACAGAGATATGGAGGTTTTGG
CTGAAAACGATGCGGCTATGCATGCTGATATATCAGGACACCCGGCTCGTTTTGCCGTTCGAATGATTCATTCCTATGAT
TTGCTGGATGATGATCTGGAAGAAAGAACGGAAGAAAAAAGCGAGCGCTTAGAAGCGATCCGCAAAGGTGAACCAGTTCC
CGGCTCGTTTGCATTTGATGAAAAAGCCCGCGAGCAGCTGAGCTGGACATACCCGCATCACGAGGTCACGCAAATTCGTA
CAAAACAGTCGGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCCGGGCCCCCGTTAAACCTGCT
GACGGAAGCATTCTGTACAGGCGTCCCGCTTTTATGATGAAAAAAGGCCTGACAGCGGCGGAGAAAGGGACGGCCATGCA
TACGGTTATGCAGCATATCCCGCTTACACATATCCCAACGATAGAAGAAGCGGAGCAGACGGTTCACAAGCTTTATGAAA
AAGAACTGCTCACTGAAGAACAAAAAAACGCTATTGATATAGAAGAAATCGTGCAATTTTTCCATACAGAAATCGGCGGA
CAGCTGATCGGCGCTAAATGGAAGGACCGGGAAGTGCCATTTAGTTTAGCGCTTCCGGCCAAGGAGATCTATCCTGATGC
ACAGGAGGCGGATGAACCGCTTTTAGTGCAGGGAATCATCGACTGTCTCTATGAAACTGAGGACGGCTTATATCTCTTGG
ACTATAAATCAGACCGGATTGAGGGCAAATTCCAGCACGGCTTTGAAGGAGCAGCCCCGATCTTGAAGAAACGATATGAA
ACGCAAATTCAGCTGTACACGAAGGCAGTTGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTGACATTATAG

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

96.677

100

0.967

Multiple sequence alignment