Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   ACK2WG_RS05790 Genome accession   NZ_CP176536
Coordinates   1131821..1135321 (+) Length   1166 a.a.
NCBI ID   WP_409509595.1    Uniprot ID   -
Organism   Bacillus spizizenii strain 29-4     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1126821..1140321
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2WG_RS05770 (ACK2WG_05770) - 1127675..1128877 (-) 1203 WP_072181834.1 MDR family MFS transporter -
  ACK2WG_RS05775 (ACK2WG_05775) - 1128991..1130718 (+) 1728 WP_409509594.1 ABC transporter substrate-binding protein -
  ACK2WG_RS05780 (ACK2WG_05780) - 1130754..1131083 (-) 330 WP_041520913.1 four-helix bundle copper-binding protein -
  ACK2WG_RS05785 (ACK2WG_05785) - 1131200..1131637 (-) 438 WP_014113300.1 ferritin family protein -
  ACK2WG_RS05790 (ACK2WG_05790) addB 1131821..1135321 (+) 3501 WP_409509595.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ACK2WG_RS05795 (ACK2WG_05795) addA 1135308..1139012 (+) 3705 WP_409509596.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ACK2WG_RS05800 (ACK2WG_05800) sbcD 1139084..1140259 (+) 1176 WP_041904943.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134761.70 Da        Isoelectric Point: 5.4257

>NTDB_id=1080760 ACK2WG_RS05790 WP_409509595.1 1131821..1135321(+) (addB) [Bacillus spizizenii strain 29-4]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMAESGTASEYRGERVLSE
KLHDLSILYQQMEKSLADQYLHSEDYLTLLAEHIPFAEDIKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLDITYKELSGTERHTQTPELAHLEAQYEARPAIPYAEKQEAFTVMQAANR
RAELEGIAREIHALVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSIDVLKGNWRY
EAVFRCVKTELLFPLNEPKTKVREQVDQLENYCIAYGIKGDRWTKGDRFHYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPQLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLLEEFVEMMGDEEI
SLDLFQQMMEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVLSDDDREWLKTVGIELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSIVIKRLEELFPQHKERLLTNEPEQVSDEEQLMYVVNKS
VAQSFTASQLRLWTREYDISDVWWSTYNVLMSEPDRLQSKNLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKMDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFKLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEAVRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFELLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRPLKAEKDKTILEWIKKEADGNEHS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=1080760 ACK2WG_RS05790 WP_409509595.1 1131821..1135321(+) (addB) [Bacillus spizizenii strain 29-4]
TTGGGAGCAGAGTTTTTAGTAGGCAGATCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGCATTCAGGATGAACTGCG
CCGGGCTCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCTGATATGGGCGGAATGATACGTGCTCAAGTGTTCAGCTTTTCACGATTGGCCTGGCGCGTCCTTCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTTACGAGCACAGGCGTACAGATGCTCCTGCGGAAGCTCATTGAGGAGCATAAACAGGAGTT
TAAAGTCTATCAAAAAGCAAGTGACAAAAGCGGATTTACCGCACAGGTTGAACGGATGCTGACAGAATTTAAGCGTTACT
GCCTGGAACCGGAAGATATCCGCCGGATGGCGGAAAGCGGAACGGCTTCCGAGTATCGCGGGGAACGTGTTTTATCTGAA
AAGCTTCATGATTTATCAATTCTGTATCAGCAGATGGAAAAAAGCCTTGCAGATCAATATCTTCACTCTGAGGATTATTT
GACATTGCTGGCAGAGCATATTCCGTTTGCGGAAGATATAAAAGGCGCGCATATTTATGTGGACGGCTTTTATCAATTTA
CACCGCAGGAATTCAGGGTCTTGGAGCAGCTTATGGTTCATGCAGAGCACATCACGTTTTCGCTCACAGCGGACAAGCCG
TCATATGAGCGGGAGCCGCATGAACTGGAATTGTTCAGAATGACGGGAAAAACGTATTATCGCCTGCATCAGAAGGCGAA
GGAACTGAATCTGGACATTACCTATAAAGAGCTAAGCGGGACTGAGCGGCATACGCAGACGCCGGAACTAGCACATCTAG
AGGCGCAGTATGAAGCGCGACCGGCCATCCCGTACGCAGAAAAACAAGAGGCCTTTACTGTGATGCAGGCCGCAAACAGA
CGAGCTGAACTGGAAGGCATTGCCCGGGAAATTCACGCTCTTGTCAGAGAGAAGGGCTATCGCTATAAAGATGTAGCGAT
TCTTGCGCGCCAGCCGGAAGACTACAAGGATATGGTGAAGGAAGTTTTCGCAGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCTTCTATGCTGAACCATCCGTTAATCGAATTTATCCGGTCGAGCATTGATGTTCTGAAAGGGAATTGGCGTTAT
GAAGCGGTGTTTCGCTGCGTGAAAACCGAACTGCTATTCCCGCTTAACGAGCCGAAAACTAAGGTGAGAGAACAGGTCGA
TCAGCTTGAAAATTACTGTATCGCCTACGGGATCAAAGGCGATCGCTGGACAAAGGGCGACCGTTTCCATTACAGACGTT
TTGTGTCATTGGATGATGATTTTGCGCAAACCGATCAGGAAATCGAAATGGAAAACATGCTGAATGACACACGCGACTGG
ATTGTTCCGCAGCTTTTTCAGCTTCAAAAACGAATGAAAAAAGCAAAGACGGTTCAGGAGAAGGCGGAGGCGCTCTACCG
CTATCTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGACAGCGTGCTGAGGAAGACGGCAGAATTATTGAAG
CGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGAGATGAAGAGATT
TCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCA
GGTATTTGTCGGCAATATGGATTTATCCAGAATGTATGGCACCTCCTGCACCTTTGTGTTGGGGGCAAACGACGGCGTTC
TGCCTGCACGCCCCGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGGTTGGGATTGAACTTTCC
TCAGGCGGGCGGGAGCGTCTGCTTGATGAGCATTTCCTCATCTATATGGCGTTTTCCAGTCCGTCTGACCGGCTTTACGT
ATCGTATCCGATTGCCGATGCGGAAGGAAAAACGCTTTTGCCATCGATTGTCATTAAACGGCTGGAAGAACTGTTTCCGC
AGCATAAGGAGCGTCTGTTGACAAATGAACCTGAACAGGTCAGCGATGAGGAACAGCTTATGTATGTCGTCAATAAAAGC
GTGGCACAGTCCTTTACCGCAAGCCAGCTCAGATTATGGACTAGAGAATACGACATCAGCGACGTCTGGTGGAGCACGTA
CAATGTGCTGATGAGTGAGCCGGACAGGCTGCAATCGAAAAATCTGTTCTCAAGCCTGTTTTTCCGGAATGAAGTGAAGC
AGCTTGAACGCAGCGTGTCGAGACAGCTTTATGGTGAACGCATTCAGGGCAGTGTATCGAGAATGGAAACCTTTAACGCG
TGCCCGTTCTCCCATTTTGCATCACACGGGCTGCATCTGAAGGAACGGCAATTTTTCAAGCTCGAAGCACCGGATATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCTGACCGGCTGCGCGAGCAAAAAATGGATTGGCGCGATTTAACGAAAG
AGCAGTGCGAGCTGTTTTCCTATGATGCGGTAGAGCGGCTGGCGCCGAAACTGCAAAAGGAAATTTTGCTCAGCTCAAAC
CGGCATTATTATGTAAAGGAAAAACTGCAAAAAATTGTGACACGCGTGTCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTTGTGCCGATCGGGCTTGAACTGGGCTTTGGAGGAAAAGGGCCGCTTCCGCCGCTGACCTTTAAGCTGAAAAACG
GCTGCACGATGGAACTCGTCGGGCGAATTGACCGCGTAGATAAAGCGGAAAGCTCGAAAGGCCTGCTCCTAAGGATTGTC
GATTATAAATCAAGCGACAAAGGCCTTGACTTAGCGGAAGTATATTACGGACTGGCGCTGCAAATGCTGACGTATCTTGA
TTTATCAATTACTCATTCAGCTGACTGGCTCGGAATGAGGGCGACGCCTGCCGGAGTGCTATATTTCCATATTCATGACC
CGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTG
CTCCTCGGCGATCAGGAAGCGGTTCGCCTCATGGATACGACCCTTCAAGAGGGACGGTCGAATATCATCAACGCGGGCTT
GAAGAAAGACGGCTCTCTCAGATCGGATTCAGCGGCAGTCGGCGAGAAGGAATTTGAGCTGTTGACGAAGCATGTGCGCC
GCACCTTCCAAGAAGCAGGCGAACAAATCACTGACGGACGCGTATCGATTGAGCCGTATAAAATGAAGAACAAGACGCCG
TGCACATACTGTGCGTTCAAATCAGTATGCCAATTTGATGAGTCATTAGAAGAAAACGAGTATCGCCCGTTAAAGGCTGA
AAAGGATAAGACGATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

98.027

100

0.98

Multiple sequence alignment