Detailed information NTG0108058

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AACJ33_RS02890
• Genome accession: NZ_AP029014
• Coordinates: 594671..595552 (-)
• Length: 293 a.a.
• NCBI ID: WP_075463374.1
• Uniprot ID: -
• Organism: Ralstonia syzygii subsp. indonesiensis strain MAFF 211271
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 589671..600552

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AACJ33_RS02860 (MAFF211271_05460)	argJ	590380..591609 (+)	1230	WP_013211398.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
AACJ33_RS02865 (MAFF211271_05470)	-	591625..592509 (+)	885	WP_013211399.1	ATP-binding protein	-
AACJ33_RS02870 (MAFF211271_05480)	-	592487..592948 (+)	462	WP_075463372.1	NUDIX domain-containing protein	-
AACJ33_RS02875 (MAFF211271_05490)	-	592941..593156 (-)	216	WP_013211401.1	DNA gyrase inhibitor YacG	-
AACJ33_RS02880 (MAFF211271_05500)	zapD	593167..593925 (-)	759	WP_013211402.1	cell division protein ZapD	-
AACJ33_RS02885 (MAFF211271_05510)	coaE	594043..594657 (-)	615	WP_075463373.1	dephospho-CoA kinase	-
AACJ33_RS02890 (MAFF211271_05520)	pilD	594671..595552 (-)	882	WP_075463374.1	A24 family peptidase	Machinery gene
AACJ33_RS02895 (MAFF211271_05530)	-	595555..596820 (-)	1266	WP_118882585.1	type II secretion system F family protein	-
AACJ33_RS02900 (MAFF211271_05540)	pilF	596865..598592 (-)	1728	WP_118882586.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AACJ33_RS02905 (MAFF211271_05550)	-	598840..600117 (-)	1278	WP_075463377.1	HlyC/CorC family transporter	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31667.69 Da
• Isoelectric Point: 8.0336

>NTDB_id=108058 AACJ33_RS02890 WP_075463374.1 594671..595552(-) (pilD) [Ralstonia syzygii subsp. indonesiensis strain MAFF 211271]
MSGMFVIPTLAQLPAWFVIGSAALLGLLVGSFLNVVIHRLPRMIEREEANYIAELRDEPLPHPEPYNLVIPRSACPSCGH
QIKAVENIPVISWLALRGRCSACKAPISWRYPAVELVTGVLTGACLWHFGPTWVAVASAALLWFLIAGSMIDADTQLLPD
AITQPLLWLGLLVNLFDMFARLPDAVIGAMAGYLFLWTIYWAYKLMRGREGMGYGDFKLMAALGAWFGWQALPLLVLLSS
VVGLVFGAVRIARGAKSESPFSFGPFIAGAGVIALLAGPQLVALTGLAPLLAR

Nucleotide

• Length: 882 bp

>NTDB_id=108058 AACJ33_RS02890 WP_075463374.1 594671..595552(-) (pilD) [Ralstonia syzygii subsp. indonesiensis strain MAFF 211271]
ATGTCCGGGATGTTTGTCATTCCCACGCTGGCGCAGTTGCCGGCGTGGTTCGTGATCGGCTCGGCGGCGCTGCTGGGCCT
GCTGGTCGGCAGCTTCCTCAACGTGGTGATCCACCGCCTGCCGCGCATGATCGAGCGCGAAGAAGCCAACTACATCGCCG
AGCTGCGCGACGAACCGCTGCCGCATCCCGAGCCGTACAACCTGGTCATACCGCGCTCGGCCTGCCCGTCGTGCGGCCAC
CAGATCAAGGCGGTCGAGAACATCCCGGTGATCAGCTGGCTGGCATTGCGCGGCCGCTGCAGCGCCTGCAAGGCGCCCAT
TTCCTGGCGCTACCCTGCCGTGGAGCTGGTGACCGGCGTGCTGACCGGTGCCTGCCTGTGGCATTTCGGGCCGACCTGGG
TGGCGGTGGCCTCGGCCGCGCTGTTGTGGTTCCTGATCGCCGGCTCGATGATCGACGCCGACACGCAACTGCTGCCCGAT
GCCATTACCCAGCCGCTGCTGTGGCTCGGCCTGCTGGTGAATCTGTTCGACATGTTCGCGCGGCTGCCGGATGCGGTGAT
CGGCGCGATGGCCGGGTATCTGTTCCTGTGGACCATCTACTGGGCCTACAAGCTGATGCGCGGCCGGGAAGGCATGGGCT
ACGGCGATTTCAAGCTGATGGCCGCGCTGGGTGCCTGGTTCGGCTGGCAGGCGCTGCCGCTGCTGGTACTGCTGTCGTCG
GTGGTCGGTCTGGTGTTCGGGGCGGTGCGCATCGCGCGCGGCGCCAAGAGCGAATCGCCGTTTTCGTTCGGCCCGTTCAT
CGCGGGTGCCGGCGTCATCGCCCTGCTGGCCGGCCCGCAGCTGGTGGCATTGACGGGGCTCGCGCCGCTGCTGGCGCGGT
AA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (140-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (24-129)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.208	90.444	0.481
pilD	Vibrio campbellii strain DS40M4	49.458	94.539	0.468
pilD	Neisseria gonorrhoeae MS11	48.881	91.468	0.447
pilD	Acinetobacter nosocomialis M2	46.241	90.785	0.42
pilD	Acinetobacter baumannii D1279779	46.241	90.785	0.42