Detailed information    

insolico Bioinformatically predicted

Overview

Name   epdJ   Type   Machinery gene
Locus tag   ACKUB1_RS06485 Genome accession   NZ_CP176366
Coordinates   1339759..1340694 (+) Length   311 a.a.
NCBI ID   WP_109941311.1    Uniprot ID   A0A2V2N9E5
Organism   Methanospirillum stamsii strain Pt1     
Function   DNA uptake (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1334759..1345694
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKUB1_RS06455 (ACKUB1_06455) - 1335212..1335484 (+) 273 WP_109941317.1 co-chaperone YbbN -
  ACKUB1_RS06460 (ACKUB1_06460) npdG 1335487..1336137 (+) 651 WP_109941316.1 NADPH-dependent F420 reductase -
  ACKUB1_RS06465 (ACKUB1_06465) - 1336141..1336677 (+) 537 WP_109941315.1 aldolase -
  ACKUB1_RS06470 (ACKUB1_06470) - 1336683..1337060 (+) 378 WP_109941314.1 hypothetical protein -
  ACKUB1_RS06475 (ACKUB1_06475) - 1337062..1337685 (-) 624 WP_109941313.1 hypothetical protein -
  ACKUB1_RS06480 (ACKUB1_06480) epdL 1337940..1339772 (+) 1833 WP_109941312.1 type II/IV secretion system ATPase subunit Machinery gene
  ACKUB1_RS06485 (ACKUB1_06485) epdJ 1339759..1340694 (+) 936 WP_109941311.1 type II secretion system F family protein Machinery gene
  ACKUB1_RS06490 (ACKUB1_06490) epdK 1340697..1341689 (+) 993 WP_109941310.1 type II secretion system F family protein Machinery gene
  ACKUB1_RS06495 (ACKUB1_06495) - 1341727..1343790 (-) 2064 WP_109941309.1 CHASE4 domain-containing protein -
  ACKUB1_RS06500 (ACKUB1_06500) - 1343950..1345401 (-) 1452 WP_109941308.1 response regulator -

Sequence

Protein


Download         Length: 311 a.a.        Molecular weight: 34308.10 Da        Isoelectric Point: 4.7684

>NTDB_id=1079774 ACKUB1_RS06485 WP_109941311.1 1339759..1340694(+) (epdJ) [Methanospirillum stamsii strain Pt1]
MNTYERFCFSLLGSRMSAKREKYYELIENIRSARLGITFEAYLATATVTSVLVGIIGSILIALLVYLLDVPGLISFRGNV
PVDIAATINQFQGYYLVGGTILAGIVSFVVLYGITFLIYLMYPSIIAGGRRRDIESTLPYAINYLAAMSTAGITPAEVFR
QLGNSTIYGESSVEARYIILEVDIFGHDLIESLKNVSMSTPSPRMRDFLQGCIGSVAAGSNISEFFRTKAEQYTGENRQT
QKQFLDTLALIAESYVTALVAGTLFLILIQSIMAILSGESTPLMLYIIIYLIIPFGSIMFVVLIDSMTPEW

Nucleotide


Download         Length: 936 bp        

>NTDB_id=1079774 ACKUB1_RS06485 WP_109941311.1 1339759..1340694(+) (epdJ) [Methanospirillum stamsii strain Pt1]
ATGAATACTTATGAACGGTTCTGTTTCTCCCTGCTTGGGAGCCGGATGTCAGCCAAACGGGAGAAATATTATGAACTTAT
CGAAAATATCAGGTCAGCACGGTTGGGCATCACTTTTGAAGCCTATCTAGCGACCGCCACGGTAACTTCTGTCCTTGTTG
GCATCATCGGTTCAATCCTAATCGCGCTTCTGGTTTACCTGCTTGATGTTCCCGGACTGATATCATTCAGAGGAAATGTC
CCGGTGGATATTGCAGCAACCATCAATCAGTTCCAGGGATATTATCTCGTGGGTGGGACTATCCTTGCCGGGATTGTATC
TTTTGTGGTGCTCTACGGCATTACTTTCCTAATCTATCTCATGTATCCTTCGATTATCGCCGGAGGGAGAAGAAGAGATA
TTGAGTCTACGCTTCCGTATGCAATCAACTACCTCGCGGCAATGTCTACCGCGGGAATTACTCCGGCAGAGGTATTCAGA
CAGCTGGGGAACTCGACCATCTATGGTGAGTCATCGGTAGAAGCCAGGTACATCATTCTTGAAGTGGATATTTTCGGTCA
TGATCTTATCGAGTCCCTCAAGAATGTCTCCATGAGCACACCAAGTCCCCGGATGCGCGATTTTCTCCAGGGCTGCATTG
GGAGTGTTGCAGCGGGAAGTAACATTTCTGAGTTTTTCCGGACCAAGGCTGAACAATATACCGGTGAAAACAGGCAGACA
CAAAAGCAGTTTCTGGATACCCTTGCCCTTATCGCGGAAAGTTACGTGACTGCGCTGGTTGCCGGCACCCTGTTTCTGAT
CCTTATCCAGTCGATAATGGCAATTTTATCCGGTGAGTCAACTCCCCTGATGCTGTATATCATCATCTACCTGATCATCC
CCTTTGGAAGCATCATGTTCGTGGTGCTCATTGATTCCATGACGCCGGAGTGGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(142-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2V2N9E5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  epdJ Methanoculleus thermophilus strain DSM 2373

60.323

99.678

0.601

Multiple sequence alignment