Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACJ23B_RS13215 Genome accession   NZ_CP175653
Coordinates   2601512..2602252 (-) Length   246 a.a.
NCBI ID   WP_000076101.1    Uniprot ID   N9LH62
Organism   Acinetobacter baumannii strain NIH1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2596512..2607252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ23B_RS13195 (ACJ23B_13215) aroB 2597049..2598131 (-) 1083 WP_001193997.1 3-dehydroquinate synthase -
  ACJ23B_RS13200 (ACJ23B_13220) aroK 2598160..2598729 (-) 570 WP_000643712.1 shikimate kinase AroK -
  ACJ23B_RS13205 (ACJ23B_13225) pilQ 2598741..2600906 (-) 2166 WP_001017033.1 type IV pilus secretin PilQ family protein Machinery gene
  ACJ23B_RS13210 (ACJ23B_13230) pilP 2600974..2601501 (-) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  ACJ23B_RS13215 (ACJ23B_13235) pilO 2601512..2602252 (-) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACJ23B_RS13220 (ACJ23B_13240) pilN 2602249..2602890 (-) 642 WP_000201227.1 PilN domain-containing protein Machinery gene
  ACJ23B_RS13225 (ACJ23B_13245) pilM 2602890..2603948 (-) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  ACJ23B_RS13230 (ACJ23B_13250) ponA 2604111..2606666 (+) 2556 WP_000736677.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27819.59 Da        Isoelectric Point: 4.8357

>NTDB_id=1078489 ACJ23B_RS13215 WP_000076101.1 2601512..2602252(-) (pilO) [Acinetobacter baumannii strain NIH1]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=1078489 ACJ23B_RS13215 WP_000076101.1 2601512..2602252(-) (pilO) [Acinetobacter baumannii strain NIH1]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTGCAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGCATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LH62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

100

100

1
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683

Multiple sequence alignment