Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACJ23C_RS05405 Genome accession   NZ_CP175646
Coordinates   1000197..1000937 (+) Length   246 a.a.
NCBI ID   WP_000076101.1    Uniprot ID   N9LH62
Organism   Acinetobacter baumannii strain HUMC1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 995197..1005937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ23C_RS05390 (ACJ23C_05385) ponA 995783..998338 (-) 2556 WP_000736677.1 penicillin-binding protein PBP1a -
  ACJ23C_RS05395 (ACJ23C_05390) pilM 998501..999559 (+) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  ACJ23C_RS05400 (ACJ23C_05395) pilN 999559..1000200 (+) 642 WP_000201227.1 PilN domain-containing protein Machinery gene
  ACJ23C_RS05405 (ACJ23C_05400) pilO 1000197..1000937 (+) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACJ23C_RS05410 (ACJ23C_05405) pilP 1000948..1001475 (+) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  ACJ23C_RS05415 (ACJ23C_05410) pilQ 1001543..1003708 (+) 2166 WP_001017033.1 type IV pilus secretin PilQ family protein Machinery gene
  ACJ23C_RS05420 (ACJ23C_05415) aroK 1003720..1004289 (+) 570 WP_000643712.1 shikimate kinase AroK -
  ACJ23C_RS05425 (ACJ23C_05420) aroB 1004318..1005400 (+) 1083 WP_001193997.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27819.59 Da        Isoelectric Point: 4.8357

>NTDB_id=1078393 ACJ23C_RS05405 WP_000076101.1 1000197..1000937(+) (pilO) [Acinetobacter baumannii strain HUMC1]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=1078393 ACJ23C_RS05405 WP_000076101.1 1000197..1000937(+) (pilO) [Acinetobacter baumannii strain HUMC1]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTGCAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGCATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LH62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

100

100

1
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683

Multiple sequence alignment