Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACIU4M_RS02170 Genome accession   NZ_CP174375
Coordinates   365439..366509 (-) Length   356 a.a.
NCBI ID   WP_327871870.1    Uniprot ID   -
Organism   Bacillus altitudinis strain SF5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 360439..371509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIU4M_RS02120 (ACIU4M_02120) sipW 360535..361107 (-) 573 WP_017357979.1 signal peptidase I SipW -
  ACIU4M_RS02125 (ACIU4M_02125) tapA 361157..361684 (-) 528 WP_407708572.1 amyloid fiber anchoring/assembly protein TapA -
  ACIU4M_RS02130 (ACIU4M_02130) - 361937..362263 (+) 327 WP_025207617.1 DUF3889 domain-containing protein -
  ACIU4M_RS02135 (ACIU4M_02135) - 362297..362491 (-) 195 WP_327871871.1 YqzE family protein -
  ACIU4M_RS02140 (ACIU4M_02140) - 362552..362935 (-) 384 WP_024718845.1 competence type IV pilus minor pilin ComGG -
  ACIU4M_RS02145 (ACIU4M_02145) comGF 362932..363429 (-) 498 WP_039166031.1 competence type IV pilus minor pilin ComGF -
  ACIU4M_RS02150 (ACIU4M_02150) comGE 363371..363685 (-) 315 WP_039166034.1 competence type IV pilus minor pilin ComGE -
  ACIU4M_RS02155 (ACIU4M_02155) comGD 363669..364115 (-) 447 WP_039166035.1 competence type IV pilus minor pilin ComGD -
  ACIU4M_RS02160 (ACIU4M_02160) comGC 364108..364401 (-) 294 WP_024718841.1 competence type IV pilus major pilin ComGC Machinery gene
  ACIU4M_RS02165 (ACIU4M_02165) comGB 364418..365458 (-) 1041 WP_396466826.1 competence type IV pilus assembly protein ComGB -
  ACIU4M_RS02170 (ACIU4M_02170) comGA 365439..366509 (-) 1071 WP_327871870.1 competence type IV pilus ATPase ComGA Machinery gene
  ACIU4M_RS02175 (ACIU4M_02175) - 366968..367840 (-) 873 WP_035390819.1 STAS domain-containing protein -
  ACIU4M_RS02185 (ACIU4M_02185) - 368066..368446 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ACIU4M_RS02190 (ACIU4M_02190) - 368659..368901 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  ACIU4M_RS02195 (ACIU4M_02195) - 368950..369588 (-) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  ACIU4M_RS02200 (ACIU4M_02200) - 369749..369922 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  ACIU4M_RS02205 (ACIU4M_02205) - 369947..370261 (-) 315 WP_017367597.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40352.65 Da        Isoelectric Point: 8.8590

>NTDB_id=1076952 ACIU4M_RS02170 WP_327871870.1 365439..366509(-) (comGA) [Bacillus altitudinis strain SF5]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECINEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEANL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1076952 ACIU4M_RS02170 WP_327871870.1 365439..366509(-) (comGA) [Bacillus altitudinis strain SF5]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGATCATTGGCT
GTGATGCTAAGACGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCAACTGTCAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACAGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGGAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCCAGAACAGCTATTAGAGCAGCTTTGACAGGCCATTTAGTCATGAGCACACTGCAT
GCAAAAAATGCAAAAGGAGCTCTTTATCGAATGCTGGAATTTGGTGTGACGATGAATGAACTCGAACAAACAATGGTTGC
TATTGCTGCTCAGCGGTTAATCGAACTTGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAACGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGATTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAACCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.888

100

0.649

Multiple sequence alignment