Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   ACI6TB_RS06245 Genome accession   NZ_CP174270
Coordinates   1378397..1378936 (-) Length   179 a.a.
NCBI ID   WP_012341221.1    Uniprot ID   -
Organism   Histophilus somni strain HSNMG-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1373397..1383936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI6TB_RS06225 (ACI6TB_06225) - 1374828..1374974 (-) 147 WP_157665520.1 hypothetical protein -
  ACI6TB_RS06230 (ACI6TB_06230) - 1374974..1375189 (-) 216 WP_075294140.1 transposase -
  ACI6TB_RS06235 (ACI6TB_06235) - 1375298..1375555 (+) 258 WP_406812064.1 helix-turn-helix domain-containing protein -
  ACI6TB_RS06240 (ACI6TB_06240) nrdA 1376044..1378314 (+) 2271 WP_012341222.1 class 1a ribonucleoside-diphosphate reductase subunit alpha -
  ACI6TB_RS06245 (ACI6TB_06245) pilF/pilF2 1378397..1378936 (-) 540 WP_012341221.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACI6TB_RS06250 (ACI6TB_06250) - 1378967..1380085 (-) 1119 WP_012341220.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ACI6TB_RS06255 (ACI6TB_06255) - 1380573..1380788 (-) 216 Protein_1210 DDE transposase -
  ACI6TB_RS06260 (ACI6TB_06260) - 1380829..1381227 (+) 399 WP_240731007.1 hypothetical protein -
  ACI6TB_RS06265 (ACI6TB_06265) - 1381414..1381632 (+) 219 WP_041605117.1 hypothetical protein -
  ACI6TB_RS06270 (ACI6TB_06270) - 1381642..1382160 (+) 519 WP_012340661.1 hypothetical protein -
  ACI6TB_RS06275 (ACI6TB_06275) - 1382186..1382698 (+) 513 WP_406812065.1 hypothetical protein -
  ACI6TB_RS06280 (ACI6TB_06280) - 1382864..1383466 (+) 603 WP_406812066.1 hypothetical protein -
  ACI6TB_RS06285 (ACI6TB_06285) - 1383443..1383820 (-) 378 WP_075321384.1 hypothetical protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20670.59 Da        Isoelectric Point: 9.3139

>NTDB_id=1076665 ACI6TB_RS06245 WP_012341221.1 1378397..1378936(-) (pilF/pilF2) [Histophilus somni strain HSNMG-1]
MRYRTIFTFIIFSLFLSACVSQPTTGRFNKQQAAKARIELGLGYLKLRNFSQAKLNFDKALFYQPDYYLVHSAFAYLYQL
QGDISQAKSSYEKAISLDKSQGDVKNNYGAFLCSQGEFARAYELFEQALTTPNYYQQADTYANIIICAEAEHNIPRYQKF
LQLLKNIDPKRAEEIKKVS

Nucleotide


Download         Length: 540 bp        

>NTDB_id=1076665 ACI6TB_RS06245 WP_012341221.1 1378397..1378936(-) (pilF/pilF2) [Histophilus somni strain HSNMG-1]
ATGCGATATCGGACTATATTTACTTTTATTATATTCTCTCTTTTTCTTTCTGCTTGCGTTTCTCAGCCTACAACTGGTCG
CTTTAATAAGCAACAAGCTGCAAAAGCACGAATTGAGTTAGGTTTAGGTTATTTAAAATTACGCAATTTTTCTCAAGCAA
AACTTAATTTTGATAAAGCTTTATTCTATCAACCTGACTATTATTTGGTACATTCGGCTTTTGCCTATTTATATCAATTA
CAAGGTGATATTTCGCAAGCGAAATCCTCTTATGAAAAAGCCATCAGTTTGGATAAGTCGCAAGGTGATGTGAAAAATAA
TTATGGTGCATTTTTATGTAGTCAGGGCGAATTTGCTCGTGCTTATGAGTTATTTGAGCAGGCACTTACTACGCCTAATT
ATTATCAGCAAGCGGATACCTATGCAAATATCATAATTTGTGCAGAGGCGGAACATAATATTCCACGTTATCAGAAATTT
TTACAGTTACTGAAGAATATTGATCCCAAAAGAGCGGAGGAAATAAAGAAAGTTTCTTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_7)

(109-130)

Tetratricopeptide repeat (TPR_8)

(74-100)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.121

92.179

0.48
  pilF Glaesserella parasuis strain SC1401

44.068

98.883

0.436

Multiple sequence alignment