Detailed information NTG1075377

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACJEID_RS07430
• Genome accession: NZ_CP174218
• Coordinates: 1655674..1656537 (+)
• Length: 287 a.a.
• NCBI ID: WP_015444491.1
• Uniprot ID: I7HPT6
• Organism: Legionella pneumophila strain SPF650
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1650674..1661537

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJEID_RS07410 (ACJEID_07410)	-	1651167..1651493 (-)	327	WP_010947249.1	DUF1820 family protein	-
ACJEID_RS07415 (ACJEID_07415)	-	1651676..1652419 (-)	744	WP_010947250.1	methyltransferase domain-containing protein	-
ACJEID_RS07420 (ACJEID_07420)	pilB	1652678..1654402 (+)	1725	WP_010947251.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACJEID_RS07425 (ACJEID_07425)	pilC	1654408..1655628 (+)	1221	WP_010947252.1	type II secretion system F family protein	Machinery gene
ACJEID_RS07430 (ACJEID_07430)	pilD	1655674..1656537 (+)	864	WP_015444491.1	A24 family peptidase	Machinery gene
ACJEID_RS07435 (ACJEID_07435)	-	1656551..1657756 (-)	1206	WP_015444490.1	cyclic nucleotide-binding domain-containing protein	-
ACJEID_RS07440 (ACJEID_07440)	-	1657864..1658457 (-)	594	WP_010947255.1	hypothetical protein	-
ACJEID_RS07445 (ACJEID_07445)	-	1658473..1658928 (-)	456	WP_010947256.1	hypothetical protein	-
ACJEID_RS07450 (ACJEID_07450)	-	1659062..1659877 (+)	816	WP_010947257.1	pyruvate, water dikinase regulatory protein	-
ACJEID_RS07455 (ACJEID_07455)	-	1660002..1661450 (-)	1449	WP_015444489.1	bifunctional 2-methylcitrate dehydratase/aconitate hydratase	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 32769.36 Da
• Isoelectric Point: 7.8442

>NTDB_id=1075377 ACJEID_RS07430 WP_015444491.1 1655674..1656537(+) (pilD) [Legionella pneumophila strain SPF650]
MINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFHLEQRKEKIKLNLFLPRSFCPHCKAMVKA
WQNIPLLSYILLRGRCYQCDSPISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTL
GLLWIGLIANTQNVFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITGA
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDSIINWYIGYWM

Nucleotide

• Length: 864 bp

>NTDB_id=1075377 ACJEID_RS07430 WP_015444491.1 1655674..1656537(+) (pilD) [Legionella pneumophila strain SPF650]
ATGATAAATGCACTAATAATCAATTATCCATGGTTTATGTATTTAGTCGTTGGACTCTTTTCTCTAGCCGTTGGAAGTTT
GCTTAATGTGATAATCTATCGCTTACCAATTATTTTGCAGGAAGAGTGGAAAGAGCAATGCTGTGAGCTATTCCATCTTG
AACAGCGAAAAGAAAAAATAAAACTTAATTTATTTTTACCACGTTCTTTTTGTCCTCATTGTAAAGCGATGGTTAAAGCG
TGGCAGAATATTCCTCTGTTAAGCTATATTTTGTTAAGAGGACGCTGTTACCAATGTGATAGTCCAATTTCAATCCGCTA
CCCTTTTGTTGAGACGTTGACCACAGTTTTATCCTTATACGCCAGTTGGCATTTTGGGTTTACAATACAATTATTATTTG
CTTTATTAGCTATCTGGATATTAATATCACTTGTTTTTATTGATCTTGATCATCAATTACTCCCTGATAGCCTCACTTTA
GGGCTCTTATGGATTGGCCTTATTGCTAATACCCAGAATGTATTTGTTAGTCTTGACGTTGCTGTATTAAGCTGCGCTGG
CGCTTATTTAGCCTTATGGCTATTCATTAATCTGTTTTATCTGATGACAGGTAAAGTAGGTATGGGACATGGAGATTTCA
AATTATTTGCAGCATTTGGTGCCTGGCTTGGATGGATGTATTTACCATTAATTCTACTAATATCTTCAATAACGGGGGCT
ATCATTGGTTTGATTTATTTAAAGATAAATGGTAAATCAAGAGATACTGCAATACCATTTGGCCCTTTTCTTTGCATTTC
TGGATTAATAGCTATGTTTTGGGGAGACTCAATCATCAATTGGTATATAGGTTATTGGATGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-125)

• Type IV leader peptidase family (Peptidase_A24) (136-244)

3D structure

• AlphaFold DB: I7HPT6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.11	97.909	0.481
pilD	Acinetobacter nosocomialis M2	47.985	95.122	0.456
pilD	Acinetobacter baumannii D1279779	47.273	95.819	0.453
pilD	Vibrio campbellii strain DS40M4	45.324	96.864	0.439
pilD	Neisseria gonorrhoeae MS11	42.806	96.864	0.415