Detailed information NTG0107478

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: RGQ30_RS02480
• Genome accession: NZ_AP028947
• Coordinates: 547925..548764 (-)
• Length: 279 a.a.
• NCBI ID: WP_130558488.1
• Uniprot ID: -
• Organism: Limnobacter thiooxidans strain CS-K2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 542925..553764

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RGQ30_RS02455 (RGQ30_04880)	argJ	543269..544498 (+)	1230	WP_130558493.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
RGQ30_RS02460 (RGQ30_04890)	-	544515..545396 (+)	882	WP_130558492.1	ATP-binding protein	-
RGQ30_RS02465 (RGQ30_04900)	-	545405..546361 (+)	957	WP_338284683.1	Nudix family hydrolase	-
RGQ30_RS02470 (RGQ30_04910)	zapD	546412..547221 (-)	810	WP_130558490.1	cell division protein ZapD	-
RGQ30_RS02475 (RGQ30_04920)	coaE	547294..547920 (-)	627	WP_130558489.1	dephospho-CoA kinase	-
RGQ30_RS02480 (RGQ30_04930)	pilD	547925..548764 (-)	840	WP_130558488.1	prepilin peptidase	Machinery gene
RGQ30_RS02485 (RGQ30_04940)	-	548761..549999 (-)	1239	WP_130558487.1	type II secretion system F family protein	-
RGQ30_RS02490 (RGQ30_04950)	pilB	550019..551716 (-)	1698	WP_130558486.1	type IV-A pilus assembly ATPase PilB	-
RGQ30_RS02495 (RGQ30_04960)	-	551825..552817 (-)	993	WP_130558485.1	polyprenyl synthetase family protein	-
RGQ30_RS02500 (RGQ30_04970)	rplU	553033..553344 (+)	312	WP_130558484.1	50S ribosomal protein L21	-
RGQ30_RS02505 (RGQ30_04980)	rpmA	553361..553621 (+)	261	WP_105027263.1	50S ribosomal protein L27	-

Sequence

Protein

• Length: 279 a.a.
• Molecular weight: 30493.42 Da
• Isoelectric Point: 7.5667

>NTDB_id=107478 RGQ30_RS02480 WP_130558488.1 547925..548764(-) (pilD) [Limnobacter thiooxidans strain CS-K2]
MTALNSMDPMHWLILGILLGLCAGSFLNVVAYRLPIMMQREWDSDLAEAQGREPEQLPRFNLWLPASHCPACKTPLKIWH
NIPVLSFLLLRGKCGHCHSAVSWRYPIVELLCAALFAAVAYTQPPGLSGLALMAFAACLLVLAVIDLDTYLLPDSLTLPL
MWAGLIVNLLGGFVPLESAVSGAALGYLVLWFIYQAFKLFTGKEGMGYGDFKLLAAIGAWLGVGSLFSVVFFASVFGIVF
GLIIQTIRGKKKTDAFPFGPCLVMGAFAWMAGFDLTRWI

Nucleotide

• Length: 840 bp

>NTDB_id=107478 RGQ30_RS02480 WP_130558488.1 547925..548764(-) (pilD) [Limnobacter thiooxidans strain CS-K2]
TTGACAGCGCTGAACAGCATGGACCCCATGCACTGGCTGATCCTTGGCATTTTGCTGGGACTGTGTGCGGGATCATTCCT
GAATGTGGTGGCCTACCGACTGCCCATCATGATGCAACGCGAATGGGACTCAGACCTGGCCGAGGCCCAAGGCCGCGAAC
CGGAACAACTTCCACGGTTCAACCTGTGGCTGCCGGCCTCGCATTGCCCAGCGTGCAAAACACCGTTGAAAATCTGGCAC
AACATCCCGGTGTTGTCGTTCCTGCTACTGCGCGGAAAATGTGGCCATTGCCACAGCGCAGTGTCATGGCGCTATCCGAT
TGTTGAACTGTTGTGTGCCGCCCTGTTTGCGGCTGTGGCCTATACCCAACCACCCGGGCTTTCAGGATTGGCCTTGATGG
CCTTTGCTGCTTGCCTGTTGGTGCTTGCCGTGATTGACCTTGACACCTACCTGCTGCCCGACAGCCTGACCTTGCCCTTG
ATGTGGGCTGGCCTGATCGTCAACCTGCTGGGGGGCTTTGTTCCGCTGGAAAGTGCGGTGTCGGGTGCAGCCTTGGGTTA
CCTGGTGTTGTGGTTCATCTACCAGGCATTCAAACTGTTCACCGGTAAAGAAGGCATGGGCTATGGCGACTTCAAACTGC
TGGCAGCCATCGGCGCATGGCTGGGCGTTGGCTCGTTGTTCAGCGTGGTGTTTTTTGCCTCGGTCTTTGGCATTGTGTTC
GGCTTGATTATTCAGACCATTCGCGGCAAGAAGAAAACCGATGCTTTTCCATTTGGCCCCTGTCTGGTCATGGGCGCCTT
CGCCTGGATGGCCGGCTTCGATTTGACACGCTGGATTTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-121)

• Type IV leader peptidase family (Peptidase_A24) (133-242)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.923	97.133	0.495
pilD	Neisseria gonorrhoeae MS11	46.886	97.849	0.459
pilD	Vibrio campbellii strain DS40M4	45.62	98.208	0.448
pilD	Acinetobacter baumannii D1279779	44.015	92.832	0.409
pilD	Acinetobacter nosocomialis M2	43.243	92.832	0.401