Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACI3EV_RS00040 Genome accession   NZ_CP173677
Coordinates   7946..9487 (+) Length   513 a.a.
NCBI ID   WP_329769736.1    Uniprot ID   -
Organism   Brachymonas sp. G13     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2946..14487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI3EV_RS00015 (ACI3EV_00015) argS 2948..4645 (+) 1698 WP_329769731.1 arginine--tRNA ligase -
  ACI3EV_RS00020 (ACI3EV_00020) - 4656..5645 (+) 990 WP_329769732.1 SPOR domain-containing protein -
  ACI3EV_RS00025 (ACI3EV_00025) - 5762..6424 (+) 663 WP_329769733.1 thiol:disulfide interchange protein DsbA/DsbL -
  ACI3EV_RS00030 (ACI3EV_00030) lptA 6501..7076 (+) 576 WP_329769734.1 lipopolysaccharide transport periplasmic protein LptA -
  ACI3EV_RS00035 (ACI3EV_00035) lptB 7073..7840 (+) 768 WP_337691793.1 LPS export ABC transporter ATP-binding protein -
  ACI3EV_RS00040 (ACI3EV_00040) rpoN/rpoN1 7946..9487 (+) 1542 WP_329769736.1 RNA polymerase factor sigma-54 Machinery gene
  ACI3EV_RS00045 (ACI3EV_00045) ssb 9568..10098 (-) 531 WP_337691792.1 single-stranded DNA-binding protein Machinery gene
  ACI3EV_RS00050 (ACI3EV_00050) - 10255..11460 (-) 1206 WP_337691862.1 MFS transporter -
  ACI3EV_RS00055 (ACI3EV_00055) - 11591..13468 (-) 1878 WP_329769738.1 potassium transporter Kup -
  ACI3EV_RS00060 (ACI3EV_00060) - 13665..13907 (-) 243 WP_329769739.1 GlsB/YeaQ/YmgE family stress response membrane protein -

Sequence

Protein


Download         Length: 513 a.a.        Molecular weight: 57198.29 Da        Isoelectric Point: 4.7265

>NTDB_id=1073644 ACI3EV_RS00040 WP_329769736.1 7946..9487(+) (rpoN/rpoN1) [Brachymonas sp. G13]
MKAGLQLRISQNLALTPQLQHAIRLLQASTQELQQELEQMLAENPFLEQAEEDTGTDDQADPPAAATSHAEDAAERLDRD
EDWSGRDAWDDWSSGANERDSADSHASSQASEEQLHTRWASHQPDDDESPWSGKPQPDKLSTHLHQQALALRLDALDRAA
LYALVESLNEDGYLEDSLDELALALQQQIPDSLLDSDQETLHDHLRIALGLLQSMEPPGVGARDLAECLRLQLLALPASQ
TDAATRQIALDLCAQPLELLARRDLRTLARLTGHGQPALRQALQLIARLEPKPGRRFATAEQQVVIPDVLVLRAPAGQQS
PWLVRLNPAVLPRVQVQDRYAGVLRQYQGEAVQALQQQLQEARWMVKSIQQRFDTILRVSQAIVERQQRFFEQGVLAMEP
LVLREIAEALALHESTVSRATSAKYMQTPWGTFELKYFFGSGIGTDDGQATSSTAVRALIGQLIADEEVRKPLSDQRLVD
LLAERGIQCARRTVAKYREQLKLPPAHLRKSLD

Nucleotide


Download         Length: 1542 bp        

>NTDB_id=1073644 ACI3EV_RS00040 WP_329769736.1 7946..9487(+) (rpoN/rpoN1) [Brachymonas sp. G13]
ATGAAAGCCGGTCTGCAACTCCGTATCAGCCAGAACCTGGCGCTGACGCCACAGCTGCAGCACGCGATCCGCTTGCTGCA
GGCTTCGACCCAGGAGCTGCAGCAAGAGCTGGAGCAGATGCTGGCCGAGAATCCGTTTCTGGAGCAGGCCGAGGAGGATA
CCGGTACGGACGACCAAGCCGATCCGCCAGCAGCAGCCACCAGTCATGCGGAAGACGCAGCCGAACGCCTTGACCGCGAC
GAGGACTGGTCTGGGCGCGATGCCTGGGACGACTGGTCTTCAGGCGCCAACGAACGCGATAGCGCAGACAGCCACGCATC
CAGCCAGGCCAGCGAAGAGCAGCTCCATACCCGCTGGGCCAGCCACCAGCCTGATGATGACGAGTCGCCCTGGTCCGGCA
AGCCGCAGCCCGACAAGCTGAGCACCCACTTGCACCAGCAGGCGCTGGCCTTGCGGCTGGATGCCCTGGACCGCGCTGCC
CTGTATGCGCTGGTCGAATCCTTGAATGAAGACGGCTATCTGGAAGACAGCCTGGACGAGCTGGCGCTCGCGCTGCAGCA
GCAGATTCCGGACAGCCTGCTGGATAGCGACCAGGAAACCCTGCACGACCACTTGCGCATCGCACTGGGTCTGCTGCAGT
CGATGGAACCGCCTGGCGTTGGCGCACGCGACCTGGCCGAATGCCTGCGACTGCAGCTGCTGGCGCTACCCGCCAGCCAG
ACCGATGCCGCCACGCGCCAGATTGCACTGGACCTGTGCGCGCAACCGCTGGAACTGCTGGCGCGACGCGACCTGCGCAC
GCTGGCACGCCTGACCGGCCATGGCCAGCCTGCGCTGCGCCAGGCACTGCAGCTGATTGCCCGCCTCGAGCCCAAGCCAG
GCCGCCGCTTTGCCACTGCCGAGCAACAAGTGGTGATTCCCGATGTTCTGGTGCTGCGCGCTCCGGCAGGCCAGCAGTCT
CCCTGGCTGGTGCGGCTCAATCCAGCGGTGCTGCCGCGCGTGCAGGTACAGGACAGGTATGCGGGCGTGCTGCGCCAGTA
CCAGGGCGAAGCGGTGCAGGCACTGCAACAGCAGCTGCAGGAGGCGCGCTGGATGGTCAAGAGCATCCAGCAGCGCTTTG
ACACCATCCTGCGCGTCAGCCAGGCGATCGTGGAGCGCCAGCAACGCTTTTTCGAGCAAGGTGTGCTGGCCATGGAGCCG
CTGGTGCTGCGCGAGATTGCCGAGGCGCTGGCGCTACACGAATCCACTGTCAGCCGCGCCACCAGCGCCAAGTACATGCA
GACACCCTGGGGCACTTTCGAACTCAAGTATTTCTTTGGTTCCGGCATCGGCACCGATGACGGTCAGGCCACCAGCAGCA
CCGCCGTGCGCGCCCTGATCGGCCAGCTGATTGCCGATGAAGAGGTACGCAAGCCGCTCAGTGACCAGCGCCTGGTCGAC
CTGCTGGCGGAGCGCGGCATCCAGTGTGCCCGCCGCACCGTGGCCAAGTACCGTGAACAGCTCAAGCTGCCGCCGGCACA
TTTGCGCAAGTCGCTGGACTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(354-510)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-340)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.828

100

0.489

Multiple sequence alignment