Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACI5E1_RS04685 Genome accession   NZ_CP173662
Coordinates   1043597..1044976 (+) Length   459 a.a.
NCBI ID   WP_005601211.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain APL2305174E     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1038597..1049976
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI5E1_RS04655 (ACI5E1_04650) - 1038634..1039272 (-) 639 WP_005604550.1 prepilin peptidase -
  ACI5E1_RS04660 (ACI5E1_04655) - 1039299..1039796 (-) 498 Protein_909 type II secretion system F family protein -
  ACI5E1_RS04665 (ACI5E1_04660) - 1039885..1040859 (+) 975 WP_012478345.1 IS30-like element ISApl1 family transposase -
  ACI5E1_RS04670 (ACI5E1_04665) - 1040869..1041573 (-) 705 Protein_911 type II secretion system F family protein -
  ACI5E1_RS04675 (ACI5E1_04670) pilB 1041557..1042951 (-) 1395 WP_237593620.1 GspE/PulE family protein Machinery gene
  ACI5E1_RS04680 (ACI5E1_04675) pilA 1042969..1043415 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACI5E1_RS04685 (ACI5E1_04680) radA 1043597..1044976 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  ACI5E1_RS04690 (ACI5E1_04685) - 1045020..1045253 (-) 234 WP_237593621.1 YgjV family protein -
  ACI5E1_RS04695 (ACI5E1_04690) folA 1045383..1045871 (-) 489 WP_237593622.1 type 3 dihydrofolate reductase -
  ACI5E1_RS04700 (ACI5E1_04695) proB 1045958..1047049 (+) 1092 WP_237593623.1 glutamate 5-kinase -
  ACI5E1_RS04705 (ACI5E1_04700) - 1047062..1047838 (+) 777 WP_005597402.1 TSUP family transporter -
  ACI5E1_RS04710 (ACI5E1_04705) nth 1047839..1048471 (+) 633 WP_005597404.1 endonuclease III -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49678.24 Da        Isoelectric Point: 7.9094

>NTDB_id=1073600 ACI5E1_RS04685 WP_005601211.1 1043597..1044976(+) (radA) [Actinobacillus pleuropneumoniae strain APL2305174E]
MAKAPKTAYVCNDCGAEYSRWMGQCKECKSWNTVSEIRLISSKEATKSDRFSGYAGETSGKVQTLSEISLQEVPRFSSGF
NELDRVLGGGVVPGSAILIGGHPGAGKSTLLLQVMCGLSKDLPTLYVTGEESLQQVAMRANRLGLPTDNLKMLSETSVEH
ICNIADQEKPKIMVIDSIQVMHLADIQSSPGSVSQVRECASFLTRYAKTRQVAIIMVGHVTKDGTLAGPKVLEHCIDASI
LLEGESDSRFRTLRSQKNRFGAVNELGVFAMTEQGLREVKNPSAIFLSRSDEQTPGSSVMVLWEGTRPLLVEIQALVDHS
MLANPRRVAVGLDHNRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALISSFRNRALPQDLVVFGEVG
LAGEIRPVPSGQERISEAAKHGFKRAIIPHGNAPKKPIKGMEVFTVKKLSDALDILGNL

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=1073600 ACI5E1_RS04685 WP_005601211.1 1043597..1044976(+) (radA) [Actinobacillus pleuropneumoniae strain APL2305174E]
ATGGCTAAAGCACCAAAAACAGCTTATGTATGTAATGATTGCGGCGCGGAATATTCTCGTTGGATGGGGCAATGCAAAGA
ATGTAAGTCTTGGAATACCGTGAGTGAAATCAGACTTATCTCGAGTAAAGAAGCAACAAAAAGCGACCGCTTCAGCGGAT
ATGCCGGCGAAACCTCCGGTAAAGTTCAAACTTTATCCGAAATCAGCTTACAAGAAGTGCCTCGTTTTAGCAGCGGCTTT
AATGAATTGGATCGCGTACTGGGCGGCGGTGTCGTGCCGGGTTCGGCAATTTTAATCGGCGGCCATCCCGGTGCCGGTAA
ATCCACTCTGCTTTTACAAGTAATGTGCGGTTTGTCTAAAGATTTGCCGACCCTTTACGTGACCGGTGAAGAATCCCTAC
AACAAGTGGCGATGCGAGCGAATCGTTTAGGCTTACCGACCGACAATCTCAAGATGCTTTCAGAAACTTCGGTCGAGCAT
ATTTGTAATATCGCCGACCAAGAAAAGCCGAAAATTATGGTAATCGACTCAATTCAGGTCATGCACCTTGCCGATATTCA
ATCTTCTCCCGGTAGCGTTTCGCAAGTGCGGGAATGCGCTTCGTTTCTGACTCGCTATGCAAAAACCCGCCAAGTAGCGA
TTATTATGGTCGGACACGTCACCAAAGACGGGACACTTGCCGGCCCGAAAGTGTTGGAACACTGTATTGACGCCTCAATT
TTACTGGAAGGCGAATCGGACTCTCGATTCCGTACCTTACGCAGCCAGAAAAACCGTTTCGGTGCGGTCAATGAATTGGG
CGTATTTGCCATGACCGAACAAGGCTTACGAGAAGTAAAAAACCCTTCCGCGATTTTCCTCAGCCGTAGCGATGAACAAA
CGCCGGGCAGTTCCGTGATGGTGCTTTGGGAAGGAACCCGTCCGTTATTAGTCGAAATTCAAGCGTTGGTTGATCATTCG
ATGTTGGCTAATCCTCGCCGTGTCGCCGTAGGTTTAGATCACAACCGTTTATCGCTCTTACTTGCGGTGTTACATCGCCA
CGGCGGTTTGCAAATGTCGGATCAAGATGTGTTCGTCAATGTGGTCGGCGGGGTAAAAGTGACCGAGACCAGTGCCGATC
TCGCCCTAATTTTGGCGTTAATTTCCAGTTTTAGAAATCGTGCGTTACCGCAAGATTTGGTCGTATTCGGCGAGGTGGGA
TTAGCCGGAGAAATTCGTCCGGTACCGAGCGGTCAAGAACGTATCAGCGAAGCGGCGAAGCACGGTTTTAAACGTGCGAT
TATTCCTCACGGCAACGCACCTAAAAAACCGATTAAAGGTATGGAAGTGTTTACCGTTAAAAAATTAAGCGACGCACTGG
ATATCCTCGGCAATCTCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(83-224)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(347-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.359

99.564

0.481
  radA Streptococcus mitis NCTC 12261

44.493

98.911

0.44
  radA Streptococcus mitis SK321

44.273

98.911

0.438
  radA Streptococcus pneumoniae Rx1

45.581

93.682

0.427
  radA Streptococcus pneumoniae D39

45.581

93.682

0.427
  radA Streptococcus pneumoniae R6

45.581

93.682

0.427
  radA Streptococcus pneumoniae TIGR4

45.581

93.682

0.427

Multiple sequence alignment