Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACI5E1_RS04680 Genome accession   NZ_CP173662
Coordinates   1042969..1043415 (-) Length   148 a.a.
NCBI ID   WP_005597397.1    Uniprot ID   B0BPG5
Organism   Actinobacillus pleuropneumoniae strain APL2305174E     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1037969..1048415
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI5E1_RS04650 (ACI5E1_04645) coaE 1037974..1038612 (-) 639 WP_237593619.1 dephospho-CoA kinase -
  ACI5E1_RS04655 (ACI5E1_04650) - 1038634..1039272 (-) 639 WP_005604550.1 prepilin peptidase -
  ACI5E1_RS04660 (ACI5E1_04655) - 1039299..1039796 (-) 498 Protein_909 type II secretion system F family protein -
  ACI5E1_RS04665 (ACI5E1_04660) - 1039885..1040859 (+) 975 WP_012478345.1 IS30-like element ISApl1 family transposase -
  ACI5E1_RS04670 (ACI5E1_04665) - 1040869..1041573 (-) 705 Protein_911 type II secretion system F family protein -
  ACI5E1_RS04675 (ACI5E1_04670) pilB 1041557..1042951 (-) 1395 WP_237593620.1 GspE/PulE family protein Machinery gene
  ACI5E1_RS04680 (ACI5E1_04675) pilA 1042969..1043415 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACI5E1_RS04685 (ACI5E1_04680) radA 1043597..1044976 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  ACI5E1_RS04690 (ACI5E1_04685) - 1045020..1045253 (-) 234 WP_237593621.1 YgjV family protein -
  ACI5E1_RS04695 (ACI5E1_04690) folA 1045383..1045871 (-) 489 WP_237593622.1 type 3 dihydrofolate reductase -
  ACI5E1_RS04700 (ACI5E1_04695) proB 1045958..1047049 (+) 1092 WP_237593623.1 glutamate 5-kinase -
  ACI5E1_RS04705 (ACI5E1_04700) - 1047062..1047838 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15980.38 Da        Isoelectric Point: 7.6257

>NTDB_id=1073599 ACI5E1_RS04680 WP_005597397.1 1042969..1043415(-) (pilA) [Actinobacillus pleuropneumoniae strain APL2305174E]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCASN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=1073599 ACI5E1_RS04680 WP_005597397.1 1042969..1043415(-) (pilA) [Actinobacillus pleuropneumoniae strain APL2305174E]
ATGCAAAAATTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACAGTAACGGGGAA
AGGGAATCTACAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGCATCAAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B0BPG5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689
  pilA Haemophilus influenzae 86-028NP

55.102

99.324

0.547
  pilA Haemophilus influenzae Rd KW20

53.061

99.324

0.527

Multiple sequence alignment