Detailed information NTG1073304

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACI516_RS14760
• Genome accession: NZ_CP173625
• Coordinates: 3256662..3257507 (-)
• Length: 281 a.a.
• NCBI ID: WP_040031453.1
• Uniprot ID: -
• Organism: Pectobacterium carotovorum strain M8a
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3251662..3262507

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACI516_RS14735 (ACI516_14730)	-	3251853..3252623 (-)	771	WP_405078371.1	ABC transporter ATP-binding protein	-
ACI516_RS14740 (ACI516_14735)	-	3252625..3253662 (-)	1038	Protein_2891	FecCD family ABC transporter permease	-
ACI516_RS14745 (ACI516_14740)	-	3253659..3254723 (-)	1065	WP_226294973.1	ABC transporter substrate-binding protein	-
ACI516_RS14750 (ACI516_14745)	-	3255046..3256071 (+)	1026	WP_010302944.1	LLM class flavin-dependent oxidoreductase	-
ACI516_RS14755 (ACI516_14750)	ypdK	3256427..3256501 (+)	75	WP_099606229.1	membrane protein YpdK	-
ACI516_RS14760 (ACI516_14755)	pilD	3256662..3257507 (-)	846	WP_040031453.1	A24 family peptidase	Machinery gene
ACI516_RS14765 (ACI516_14760)	-	3257597..3258343 (-)	747	WP_405078372.1	type II secretion system protein N	-
ACI516_RS14770 (ACI516_14765)	gspM	3258333..3258827 (-)	495	WP_010302957.1	type II secretion system protein GspM	-
ACI516_RS14775 (ACI516_14770)	gspL	3258824..3260101 (-)	1278	WP_039507901.1	type II secretion system protein GspL	-
ACI516_RS14780 (ACI516_14775)	gspK	3260098..3261084 (-)	987	WP_039471195.1	type II secretion system minor pseudopilin GspK	-
ACI516_RS14785 (ACI516_14780)	gspJ	3261081..3261731 (-)	651	WP_180775510.1	type II secretion system minor pseudopilin GspJ	-
ACI516_RS14790 (ACI516_14785)	gspI	3261731..3262096 (-)	366	WP_180775509.1	type II secretion system minor pseudopilin GspI	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31327.25 Da
• Isoelectric Point: 6.4529

>NTDB_id=1073304 ACI516_RS14760 WP_040031453.1 3256662..3257507(-) (pilD) [Pectobacterium carotovorum strain M8a]
MDDLREFAQLFPVWWFGALGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGVADSDPDTRYNLWWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQSVSVQYPLVEVITMLAFLAAGLLWLPGMALWGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWMGLLFNLSGTFVSLNDAVVGAMAGYLSLWLLYWAFKYVTGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLIWRGLRKEDTAKPLAFGPWLAIGGVFGVIMNGFNL

Nucleotide

• Length: 846 bp

>NTDB_id=1073304 ACI516_RS14760 WP_040031453.1 3256662..3257507(-) (pilD) [Pectobacterium carotovorum strain M8a]
GTGGACGATTTAAGGGAATTCGCACAACTTTTTCCGGTGTGGTGGTTTGGGGCGCTGGGCGTGTTGGGGTTGATTGTTGG
CAGTTTTCTGAACGTGGTGATTTACCGCTTGCCGATTATGCTGGAGCGTCGCTGGCGGCAGGACATCGAGCTTGAAACGG
GCGTGGCCGATTCCGATCCTGATACGCGCTACAACCTGTGGTGGCCGCCTTCGTCTTGCCCGCACTGCCAGCAGGCTATT
GCGGTCAAAGATAATATCCCGCTATTCAGCTGGCTGTGGCTGCGCGGCCGCTCCCGCTGCTGCCATCAATCGGTCTCTGT
GCAATATCCGTTAGTGGAAGTCATCACCATGCTGGCTTTTCTGGCGGCAGGTTTGCTCTGGTTGCCCGGTATGGCGCTGT
GGGGGGCGTTGATCCTGCTGTCCTTCCTGTTGGTGCTGACCGTTATTGATATAAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATGGGGTTGCTGTTTAATCTGTCGGGGACATTTGTCTCATTGAATGATGCCGTAGTCGGCGC
GATGGCCGGTTATTTATCTCTGTGGCTACTTTATTGGGCATTCAAATACGTAACGGGCAAAGAAGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCGCTGGGGGCTTGGCTTGGCTGGCAGGCATTGCCCAATCTGGTTTTGGTGGCGGCGCTGAGT
GGGCTGGTCGTGACTCTCATCTGGCGCGGGTTGCGTAAAGAGGATACGGCTAAACCGCTGGCCTTTGGCCCCTGGCTGGC
AATCGGCGGGGTGTTCGGCGTGATAATGAACGGATTCAATCTGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.725	98.221	0.498
pilD	Vibrio campbellii strain DS40M4	47.037	96.085	0.452
pilD	Neisseria gonorrhoeae MS11	46.36	92.883	0.431
pilD	Acinetobacter baumannii D1279779	44.358	91.459	0.406
pilD	Acinetobacter nosocomialis M2	43.969	91.459	0.402