Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   AAA995_RS10680 Genome accession   NZ_AP028877
Coordinates   2074961..2076259 (-) Length   432 a.a.
NCBI ID   WP_000867591.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 21P20     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2069961..2081259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAA995_RS10660 (SP21P20_20690) rplI 2071116..2071568 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  AAA995_RS10665 (SP21P20_20700) - 2071565..2073538 (-) 1974 WP_000715116.1 DHH family phosphoesterase -
  AAA995_RS10670 (SP21P20_20710) raiA 2073674..2074222 (-) 549 WP_000599107.1 ribosome-associated translation inhibitor RaiA -
  AAA995_RS10675 (SP21P20_20720) comFC/cflB 2074302..2074964 (-) 663 WP_000649971.1 ComF family protein Machinery gene
  AAA995_RS10680 (SP21P20_20730) comFA/cflA 2074961..2076259 (-) 1299 WP_000867591.1 DEAD/DEAH box helicase Machinery gene
  AAA995_RS10685 (SP21P20_20740) - 2076315..2076950 (+) 636 WP_000395738.1 YigZ family protein -
  AAA995_RS10690 (SP21P20_20750) cysK 2077235..2078155 (+) 921 WP_000029866.1 cysteine synthase A -
  AAA995_RS10695 - 2078230..2078759 (-) 530 Protein_2068 transposase -
  AAA995_RS10700 (SP21P20_20770) - 2078741..2079049 (+) 309 Protein_2069 transposase -
  AAA995_RS10705 (SP21P20_20780) tnpB 2079190..2079405 (-) 216 WP_000376909.1 IS66 family insertion sequence element accessory protein TnpB -
  AAA995_RS10710 (SP21P20_20790) tsf 2079760..2080800 (-) 1041 WP_000808062.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49562.56 Da        Isoelectric Point: 8.5992

>NTDB_id=107239 AAA995_RS10680 WP_000867591.1 2074961..2076259(-) (comFA/cflA) [Streptococcus pneumoniae strain 21P20]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSIILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKATKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=107239 AAA995_RS10680 WP_000867591.1 2074961..2076259(-) (comFA/cflA) [Streptococcus pneumoniae strain 21P20]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGCTGTAATAGTATTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGAATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCATGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAATTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGCAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGACTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(293-396)

Type III restriction enzyme, res subunit (ResIII)

(94-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.537

100

0.995
  comFA/cflA Streptococcus pneumoniae D39

99.537

100

0.995
  comFA/cflA Streptococcus pneumoniae R6

99.537

100

0.995
  comFA/cflA Streptococcus pneumoniae TIGR4

99.306

100

0.993
  comFA/cflA Streptococcus mitis NCTC 12261

93.056

100

0.931
  comFA/cflA Streptococcus mitis SK321

92.13

100

0.921
  comFA Lactococcus lactis subsp. cremoris KW2

52.696

94.444

0.498
  comFA Latilactobacillus sakei subsp. sakei 23K

38.979

99.769

0.389

Multiple sequence alignment