Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACI1GX_RS06665 Genome accession   NZ_CP173358
Coordinates   1475198..1475746 (-) Length   182 a.a.
NCBI ID   WP_237593297.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain APL2305225E     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1470198..1480746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI1GX_RS06650 (ACI1GX_06640) - 1470680..1471201 (+) 522 WP_193327846.1 SprT family zinc-dependent metalloprotease -
  ACI1GX_RS06655 (ACI1GX_06645) - 1471306..1472592 (+) 1287 WP_252590385.1 NAD(P)/FAD-dependent oxidoreductase -
  ACI1GX_RS06660 (ACI1GX_06650) - 1472784..1475123 (-) 2340 WP_237593296.1 Tex family protein -
  ACI1GX_RS06665 (ACI1GX_06655) pilF 1475198..1475746 (-) 549 WP_237593297.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACI1GX_RS06670 (ACI1GX_06660) - 1475800..1476981 (-) 1182 WP_005608495.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ACI1GX_RS06695 (ACI1GX_06685) gltX 1477887..1479326 (+) 1440 WP_237592596.1 glutamate--tRNA ligase -
  ACI1GX_RS06705 (ACI1GX_06695) yefM 1479664..1479915 (+) 252 WP_017357689.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  ACI1GX_RS06710 (ACI1GX_06700) - 1479912..1480172 (+) 261 WP_237593298.1 Txe/YoeB family addiction module toxin -
  ACI1GX_RS06715 (ACI1GX_06705) - 1480331..1480498 (-) 168 WP_005598320.1 Trm112 family protein -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20643.25 Da        Isoelectric Point: 6.6300

>NTDB_id=1071596 ACI1GX_RS06665 WP_237593297.1 1475198..1475746(-) (pilF) [Actinobacillus pleuropneumoniae strain APL2305225E]
MNLAKFLRNLSACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLDQHDLPNAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQVHAEQAYQQALKLSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSLENIVLCAKQEPNL
AKVNEALSQLEKLDKSRAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=1071596 ACI1GX_RS06665 WP_237593297.1 1475198..1475746(-) (pilF) [Actinobacillus pleuropneumoniae strain APL2305225E]
ATGAATCTTGCAAAATTTTTGCGAAATTTGAGCGCTTGTATGCTGTTATCCGTGTTGTTCGGTTGTTCTTCTCAGCTTCC
TACCTCCCAAACTACCTTTAATCGTTCGGAAGCGGTTAAAGCCCGTATTAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAACGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTATTTGCCTCACTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGTTCATGCGGAACAGGCTTATCAGCAAGCATTGAAATTAAGTGATAATCGTCC
GGATGTCTTAAATAATTACGGTACTTTTCTGTGTAAACAAGCCAAATTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGAAAAGCGAACAACCTTATTATCATCAAGCCGATAGCCTCGAAAATATCGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGTAAACGAAGCGTTAAGCCAATTAGAAAAGCTGGATAAATCAAGAGCGGCGGCACTACGCTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(79-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

65.957

100

0.681
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.368

93.956

0.445

Multiple sequence alignment