Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACIVJH_RS00295 Genome accession   NZ_CP173226
Coordinates   49313..50173 (+) Length   286 a.a.
NCBI ID   WP_003689814.1    Uniprot ID   A0AA44U9R8
Organism   Neisseria gonorrhoeae strain NCTC 8375 [B 5025]     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 44313..55173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIVJH_RS00280 dapD 44823..45644 (-) 822 WP_003689804.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  ACIVJH_RS00285 pgi 45854..47497 (-) 1644 WP_003697594.1 glucose-6-phosphate isomerase -
  ACIVJH_RS00290 pilG 48007..49239 (+) 1233 WP_003689811.1 type 4 pilus assembly protein PilG Machinery gene
  ACIVJH_RS00295 pilD 49313..50173 (+) 861 WP_003689814.1 A24 family peptidase Machinery gene
  ACIVJH_RS00300 coaE 50170..50802 (+) 633 WP_003689816.1 dephospho-CoA kinase -
  ACIVJH_RS00305 - 50795..51004 (+) 210 WP_003696096.1 DNA gyrase inhibitor YacG -
  ACIVJH_RS00310 pilF 51090..52766 (-) 1677 WP_003689817.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACIVJH_RS00320 - 53104..53550 (-) 447 WP_003689818.1 DUF441 domain-containing protein -
  ACIVJH_RS00325 - 53554..54528 (-) 975 WP_003689819.1 polyprenyl synthetase family protein -
  ACIVJH_RS00330 rplU 54752..55060 (+) 309 WP_003691838.1 50S ribosomal protein L21 -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 31332.34 Da        Isoelectric Point: 8.9561

>NTDB_id=1070838 ACIVJH_RS00295 WP_003689814.1 49313..50173(+) (pilD) [Neisseria gonorrhoeae strain NCTC 8375 [B 5025]]
MSDLSVLSPFAVPLAAVLGLLVGSFLNVVIYRVPVMMERGWTVFAKEHLNLPLTDDESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYSSLWLLCAVYKLLTGKTGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGRHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVR

Nucleotide


Download         Length: 861 bp        

>NTDB_id=1070838 ACIVJH_RS00295 WP_003689814.1 49313..50173(+) (pilD) [Neisseria gonorrhoeae strain NCTC 8375 [B 5025]]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGGCAGCAGTTTTGGGGCTGCTGGTCGGCAGCTTCCTGAA
TGTCGTCATTTACCGCGTACCCGTTATGATGGAACGCGGCTGGACGGTATTTGCCAAAGAACATTTAAACCTGCCGCTGA
CCGACGATGAAAGCCGTACCTTCAACCTGATGAAGCCGGATTCCTGCTGTCCCAAATGCCGTGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGTTACCTGCTCCTGCGCGGCAAATGCGCTTCCTGCCAAACCAAAATCAGCATACGTTATCC
CTTAATCGAGCTGCTGACCGGCGTATTGTTCGGGCTGGTCGCCTGGCAATACGGCTGGTCTTGGATTACGCTGGGCGGTT
TGATACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACATTACCC
TTGATCTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATAGTTCATTATGGCTCTTATGTGCAGTGTATAAACTGCTCACAGGAAAAACCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCTCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCGGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGAGATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)

Type IV leader peptidase family (Peptidase_A24)

(134-242)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

100

100

1
  pilD Vibrio cholerae strain A1552

44.792

100

0.451
  pilD Vibrio campbellii strain DS40M4

45.652

96.503

0.441
  pilD Acinetobacter baumannii D1279779

47.727

92.308

0.441
  pilD Acinetobacter nosocomialis M2

47.727

92.308

0.441

Multiple sequence alignment