Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   ACIJUN_RS16840 Genome accession   NZ_CP172962
Coordinates   3589340..3590932 (+) Length   530 a.a.
NCBI ID   WP_196686611.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain YB1     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 3584340..3595932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIJUN_RS16820 (ACIJUN_16825) pgeF 3586090..3586818 (-) 729 WP_012614543.1 peptidoglycan editing factor PgeF -
  ACIJUN_RS16825 (ACIJUN_16830) rluD 3586815..3587777 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  ACIJUN_RS16830 (ACIJUN_16835) - 3587923..3588948 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  ACIJUN_RS16835 (ACIJUN_16840) - 3589105..3589350 (+) 246 WP_009316269.1 PP0621 family protein -
  ACIJUN_RS16840 (ACIJUN_16845) pilS 3589340..3590932 (+) 1593 WP_196686611.1 two-component system sensor histidine kinase PilS Regulator
  ACIJUN_RS16845 (ACIJUN_16850) pilR 3590947..3592284 (+) 1338 WP_003094694.1 two-component system response regulator PilR Regulator
  ACIJUN_RS16850 (ACIJUN_16855) thiO 3592330..3593424 (-) 1095 WP_003145981.1 glycine oxidase ThiO -
  ACIJUN_RS16855 (ACIJUN_16860) - 3593559..3594068 (+) 510 WP_003102595.1 Tfp pilus assembly protein FimT/FimU -
  ACIJUN_RS16860 (ACIJUN_16865) - 3594174..3594680 (+) 507 WP_003102598.1 Tfp pilus assembly protein FimT/FimU -
  ACIJUN_RS16865 (ACIJUN_16870) pilV 3594671..3595228 (+) 558 WP_003119423.1 type 4a pilus minor pilin PilV Machinery gene

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58998.20 Da        Isoelectric Point: 6.8189

>NTDB_id=1069652 ACIJUN_RS16840 WP_196686611.1 3589340..3590932(+) (pilS) [Pseudomonas aeruginosa strain YB1]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEDQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLSSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLG
RHSPMLMHCMKQWRLNPSLRPPTLKVVPDGPTVQPSFISLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLQRFVDEYPGRLRND
SQLHLQLGAGDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLARDPESDLPVLEIIDDGPGVPADKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREQGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=1069652 ACIJUN_RS16840 WP_196686611.1 3589340..3590932(+) (pilS) [Pseudomonas aeruginosa strain YB1]
GTGCGCGCTGAACGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGCCTGACCAT
CGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAACTGGAAGATCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCGCGGCAATTGCTGCCGATCTTC
ATCCTCGCGCTCACCGACGTGCTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTGCCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTCGCCATTGCCAACATCCTGCTGCGCGGGCGCATCGGCCTGGTCATCGCGGCGGCGGCCAGCCTCG
GCCTGCTCTACCTGACCTTCTTCCTCAGCCTGAGCAGTCCGGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCTCTGGTGCGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACG
CGCCGAGACGGTCGCCAACCTGGAGGAACTCAACGCATTGATCCTGCAGCGCATGCGCACCGGCATCCTCGTGGTCGATA
GCCGTCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTGCAGGGCGCCAGCCTGGGC
CGCCACAGCCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCGCCGACGCTCAAGGTGGT
GCCGGATGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTCGGCCGCCTGACCGCCGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCAATCAGCCACGCCGCCCAACTGCTGCAGGAGTCAGAGGAACTGGATGCCCCGGACCGACG
CCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTTCAGCGGTTCGTCGACGAATACCCCGGCAGGCTGCGCAACGAC
AGCCAACTGCACCTGCAGCTCGGTGCCGGCGACATCCAGACCCGCATGGACCCACATCAGTTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGCGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGAAATCATCGACGACGGTCCCGGCGTACCGGCGGACAAACTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAGCTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGCAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

99.623

100

0.996

Multiple sequence alignment