Detailed information NTG0106925

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AADG46_RS10910
• Genome accession: NZ_AP028804
• Coordinates: 2393529..2394545 (-)
• Length: 338 a.a.
• NCBI ID: WP_172780299.1
• Uniprot ID: -
• Organism: Vibrio cholerae strain VN152
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2388529..2399545

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AADG46_RS10885 (VNVC001_20650)	aroK	2389406..2389930 (-)	525	WP_000818616.1	shikimate kinase AroK	-
AADG46_RS10890 (VNVC001_20660)	pilQ	2390117..2391838 (-)	1722	WP_162797396.1	type IV pilus secretin PilQ family protein	Machinery gene
AADG46_RS10895 (VNVC001_20670)	pilP	2391864..2392382 (-)	519	WP_138041023.1	pilus assembly protein PilP	Machinery gene
AADG46_RS10900 (VNVC001_20680)	pilO	2392372..2392962 (-)	591	WP_000157688.1	type 4a pilus biogenesis protein PilO	Machinery gene
AADG46_RS10905 (VNVC001_20690)	pilN	2392955..2393539 (-)	585	WP_000902746.1	PilN domain-containing protein	Machinery gene
AADG46_RS10910 (VNVC001_20700)	pilM	2393529..2394545 (-)	1017	WP_172780299.1	type IV pilus assembly protein PilM	Machinery gene
AADG46_RS10915 (VNVC001_20710)	-	2394688..2397165 (+)	2478	WP_002044688.1	penicillin-binding protein 1A	-
AADG46_RS10920 (VNVC001_20720)	oxyR	2397240..2398133 (-)	894	WP_001025909.1	DNA-binding transcriptional regulator OxyR	-
AADG46_RS10925 (VNVC001_20730)	-	2398273..2399004 (+)	732	WP_000495223.1	glutathione peroxidase	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37693.41 Da
• Isoelectric Point: 7.3328

>NTDB_id=106925 AADG46_RS10910 WP_172780299.1 2393529..2394545(-) (pilM) [Vibrio cholerae strain VN152]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FVQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide

• Length: 1017 bp

>NTDB_id=106925 AADG46_RS10910 WP_172780299.1 2393529..2394545(-) (pilM) [Vibrio cholerae strain VN152]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCA
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTGTTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCAGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-221)

• Type IV pilus assembly protein PilM; (PilM_2) (240-328)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	99.704	100	0.997
pilM	Vibrio campbellii strain DS40M4	48.521	100	0.485