Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AADG46_RS10890 Genome accession   NZ_AP028804
Coordinates   2390117..2391838 (-) Length   573 a.a.
NCBI ID   WP_162797396.1    Uniprot ID   -
Organism   Vibrio cholerae strain VN152     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2385117..2396838
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AADG46_RS10870 (VNVC001_20620) - 2385860..2386693 (-) 834 WP_000744680.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  AADG46_RS10875 (VNVC001_20630) - 2386763..2388238 (-) 1476 WP_000050187.1 AAA family ATPase -
  AADG46_RS10880 (VNVC001_20640) aroB 2388293..2389378 (-) 1086 WP_000439803.1 3-dehydroquinate synthase -
  AADG46_RS10885 (VNVC001_20650) aroK 2389406..2389930 (-) 525 WP_000818616.1 shikimate kinase AroK -
  AADG46_RS10890 (VNVC001_20660) pilQ 2390117..2391838 (-) 1722 WP_162797396.1 type IV pilus secretin PilQ family protein Machinery gene
  AADG46_RS10895 (VNVC001_20670) pilP 2391864..2392382 (-) 519 WP_138041023.1 pilus assembly protein PilP Machinery gene
  AADG46_RS10900 (VNVC001_20680) pilO 2392372..2392962 (-) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  AADG46_RS10905 (VNVC001_20690) pilN 2392955..2393539 (-) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  AADG46_RS10910 (VNVC001_20700) pilM 2393529..2394545 (-) 1017 WP_172780299.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62363.46 Da        Isoelectric Point: 4.6229

>NTDB_id=106921 AADG46_RS10890 WP_162797396.1 2390117..2391838(-) (pilQ) [Vibrio cholerae strain VN152]
MKNGLKTYVAQTWLTLWVGLALCASSMVFSAESATANQLENIDFRVNKEKAAVLIVELASPSAVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVESVEVFRKEPSTQLVVTVDGEFQHDYTLKGKYLEVVISKLKADEKPKPKSVLEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLDELGVRWGFSSSNGSFGAGGSIENNLLQTVPTTLPAADFNVQDFLNVNLAATSSNASSIAFQVAK
LGSGTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPDNRLVL
DLSVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKS
ELLIFVTPKVVIQ

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=106921 AADG46_RS10890 WP_162797396.1 2390117..2391838(-) (pilQ) [Vibrio cholerae strain VN152]
ATGAAAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTATGGGTTGGTTTGGCACTTTGTGCTTCTTCCAT
GGTGTTTAGTGCTGAATCCGCTACCGCCAACCAGTTGGAAAACATCGACTTTCGGGTCAACAAAGAGAAGGCGGCGGTCT
TAATTGTCGAATTGGCTTCGCCATCGGCGGTCGTGGATGTGCAAAAAGTCCAAGAAGGGTTGAGCATTGAACTCCTGAAA
ACCGATGTGGCGGATGACAAGCTGTATCTACTTGATGTGAAAGATTTTTCGACCCCTGTTGAGAGCGTGGAAGTATTCCG
TAAAGAGCCAAGCACACAACTGGTTGTGACTGTCGATGGGGAGTTTCAGCACGATTATACCTTGAAAGGAAAATACCTTG
AGGTCGTGATCAGTAAACTCAAAGCTGATGAAAAGCCTAAGCCCAAGAGTGTCTTGGAAAAAGAAGGCAAGCTTATTTCA
ATTAACTTCCAAGATATTCCGGTGCGTAACGTACTGCAGCTGATTGCCGATTATAACGGTTTCAACTTAGTGGTTTCCGA
TTCTGTGGTGGGTAACCTGACTTTACGCTTGGATGGCGTGCCTTGGCAGCAAGTGTTGGACATTATTCTGCAAGTCAAAG
GGCTCGATAAGCGGGTCGATGGCAATGTGATTTTGATTGCGCCGAAAGAGGAACTCGACTTGCGCGAGAAACAAGCGCTC
GAAAAAGCCCGTTTAGCAGAAGAGTTGGGCGATTTAAAATCAGAAATCATCAAGATTAACTTTGCCAAAGCGTCTGACAT
TGCGGCGATGATCGGCGGTGAAGGCAACGTGAACATGTTGTCTGAACGAGGTTCGATCAGCATCGACGAACGAACTAATT
CGCTATTGATCCGAGAACTGCCCGATAATATTGCTGTGATCCGCGAGATCATTGAATCGCTGGATATTCCGGTCAAACAA
GTTCAAATCGAAGCGCGGATTGTCACCGTCAAAGAAGGTAACTTGGATGAACTAGGTGTGCGCTGGGGCTTCAGCTCGAG
CAATGGTTCATTTGGTGCTGGAGGCAGTATTGAAAACAACTTATTACAGACAGTTCCTACCACATTACCTGCTGCCGATT
TCAACGTTCAAGACTTCTTGAATGTCAACTTAGCGGCCACATCCAGCAATGCGTCTTCGATTGCTTTCCAAGTCGCTAAA
TTGGGCTCAGGTACGTTGTTGGATTTGGAGCTTTCAGCATTACAAAATGAATCGAAGGCCGAAATCATCTCTAGCCCTCG
TTTGATTACCACTAATAAACAGCCTGCATATATTGAGCAAGGTACTGAGATCCCTTATCTGGAATCGTCTTCCAGTGGTG
CCTCTACGGTCGCGTTTAAAAAAGCGGTATTGAGCTTGAAGGTGACACCACAAATCACCCCCGATAACCGCTTGGTGCTC
GACTTGAGTGTGACCCAAGACCGACGCGGTGAAACGGTGAAAACCGGTACGGGTGAAGCGGTTTCTATTGATACTCAGCG
TATTGGCACTCAAGTGTTGGTGAATAACGGAGAAACTGTGGTGTTGGGTGGCATTTTCCAACACAGCATCAATAATTCGG
TAGATAAGGTGCCACTGCTGGGGGACTTGCCTGTTTTGGGCGCGTTATTCAGACGAACCTATGAGCAAATGGGCAAAAGT
GAACTGCTGATTTTTGTCACGCCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(413-571)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

95.986

100

0.96
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

95.986

100

0.96
  pilQ Vibrio campbellii strain DS40M4

78.545

95.986

0.754
  pilQ Legionella pneumophila strain ERS1305867

42.726

94.764

0.405
  pilQ Legionella pneumophila strain Lp02

42.726

94.764

0.405
  comQ Acinetobacter baylyi ADP1

39.336

99.825

0.393
  pilQ Acinetobacter baumannii strain A118

37.755

100

0.387
  pilQ Acinetobacter baumannii D1279779

37.755

100

0.387
  pilQ Pseudomonas aeruginosa PAK

38.949

96.335

0.375

Multiple sequence alignment