Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACHGMI_RS14065 Genome accession   NZ_CP172417
Coordinates   2577502..2578572 (+) Length   356 a.a.
NCBI ID   WP_017696192.1    Uniprot ID   -
Organism   Bacillus subtilis strain AKPS2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2572502..2583572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHGMI_RS14035 yqgX 2572627..2573262 (+) 636 WP_017696186.1 MBL fold metallo-hydrolase -
  ACHGMI_RS14040 yqgY 2573302..2573547 (-) 246 WP_003226300.1 DUF2626 domain-containing protein -
  ACHGMI_RS14050 rsbRD 2573780..2574616 (+) 837 WP_017696187.1 RsbT co-antagonist protein RsbRD -
  ACHGMI_RS14055 yqhB 2574669..2575997 (-) 1329 WP_017696188.1 hemolysin family protein -
  ACHGMI_RS14060 corA 2576139..2577092 (+) 954 WP_017696189.1 magnesium transporter CorA -
  ACHGMI_RS14065 comGA 2577502..2578572 (+) 1071 WP_017696192.1 competence protein ComGA Machinery gene
  ACHGMI_RS14070 comGB 2578559..2579596 (+) 1038 WP_017696193.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACHGMI_RS14075 comGC 2579610..2579906 (+) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  ACHGMI_RS14080 comGD 2579896..2580327 (+) 432 WP_017696194.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACHGMI_RS14085 comGE 2580311..2580658 (+) 348 WP_017696195.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACHGMI_RS14090 comGF 2580684..2581067 (+) 384 WP_017696196.1 ComG operon protein ComGF Machinery gene
  ACHGMI_RS14095 comGG 2581068..2581442 (+) 375 WP_017696197.1 ComG operon protein ComGG Machinery gene
  ACHGMI_RS14100 spoIITA 2581513..2581692 (+) 180 WP_014480252.1 YqzE family protein -
  ACHGMI_RS14105 yqzG 2581734..2582060 (-) 327 WP_026113671.1 YqzG/YhdC family protein -
  ACHGMI_RS14110 tapA 2582331..2583086 (+) 756 WP_017696199.1 amyloid fiber anchoring/assembly protein TapA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40472.67 Da        Isoelectric Point: 9.0175

>NTDB_id=1067586 ACHGMI_RS14065 WP_017696192.1 2577502..2578572(+) (comGA) [Bacillus subtilis strain AKPS2]
MDSIEKISKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1067586 ACHGMI_RS14065 WP_017696192.1 2577502..2578572(+) (comGA) [Bacillus subtilis strain AKPS2]
TTGGATTCAATAGAAAAGATAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATCGTGCC
GAGGGAGCGAGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCTCAATACAATATCCCTTCGATTGATAAACTGTCGTTATTTCCAAAGACAGGAGCCACATTACTCTCATTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAC
GCAAAAAAACACTTTAATCGAAATATTGTTACATTAGAGGACCCTGTTGAAACTAGGGACGAAGACGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAGGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGATGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTGACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGGCTGCTTGAATTCGGTATCAATATGAATGAAATTGAACAGACCGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGTCCATTTTGTGAAAACGGATGTTCATCAGTATATTGCCGTCAGTCAC
GAAATACCAGAAGAGCTAGCGTTTATGAGCTTCTTTATGGGAAAAACCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

99.719

100

0.997

Multiple sequence alignment