Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACH2FV_RS11425 Genome accession   NZ_CP172339
Coordinates   2232552..2233622 (-) Length   356 a.a.
NCBI ID   WP_076840973.1    Uniprot ID   -
Organism   Bacillus safensis subsp. safensis strain KGU003     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2227552..2238622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH2FV_RS11375 (ACH2FV_11375) sipW 2227646..2228218 (-) 573 WP_041106855.1 signal peptidase I SipW -
  ACH2FV_RS11380 (ACH2FV_11380) tapA 2228271..2228798 (-) 528 WP_076840975.1 amyloid fiber anchoring/assembly protein TapA -
  ACH2FV_RS11385 (ACH2FV_11385) - 2229099..2229377 (+) 279 WP_249895874.1 DUF3889 domain-containing protein -
  ACH2FV_RS11390 (ACH2FV_11390) - 2229411..2229605 (-) 195 WP_024718846.1 YqzE family protein -
  ACH2FV_RS11395 (ACH2FV_11395) - 2229665..2230048 (-) 384 WP_103131951.1 competence type IV pilus minor pilin ComGG -
  ACH2FV_RS11400 (ACH2FV_11400) comGF 2230045..2230572 (-) 528 WP_395893902.1 competence type IV pilus minor pilin ComGF -
  ACH2FV_RS11405 (ACH2FV_11405) comGE 2230484..2230798 (-) 315 WP_041106865.1 competence type IV pilus minor pilin ComGE -
  ACH2FV_RS11410 (ACH2FV_11410) comGD 2230782..2231228 (-) 447 WP_041106867.1 competence type IV pilus minor pilin ComGD -
  ACH2FV_RS11415 (ACH2FV_11415) comGC 2231221..2231514 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  ACH2FV_RS11420 (ACH2FV_11420) comGB 2231531..2232571 (-) 1041 WP_034280202.1 competence type IV pilus assembly protein ComGB -
  ACH2FV_RS11425 (ACH2FV_11425) comGA 2232552..2233622 (-) 1071 WP_076840973.1 competence type IV pilus ATPase ComGA Machinery gene
  ACH2FV_RS11430 (ACH2FV_11430) - 2233800..2234675 (-) 876 WP_056767330.1 STAS domain-containing protein -
  ACH2FV_RS11440 (ACH2FV_11440) - 2234899..2235279 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ACH2FV_RS11445 (ACH2FV_11445) - 2235492..2235734 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  ACH2FV_RS11450 (ACH2FV_11450) - 2235781..2236419 (-) 639 WP_395893903.1 MBL fold metallo-hydrolase -
  ACH2FV_RS11455 (ACH2FV_11455) - 2236577..2236750 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  ACH2FV_RS11460 (ACH2FV_11460) - 2236775..2237089 (-) 315 WP_395893904.1 MTH1187 family thiamine-binding protein -
  ACH2FV_RS11465 (ACH2FV_11465) - 2237166..2238419 (-) 1254 WP_395893905.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40393.87 Da        Isoelectric Point: 9.3951

>NTDB_id=1066516 ACH2FV_RS11425 WP_076840973.1 2232552..2233622(-) (comGA) [Bacillus safensis subsp. safensis strain KGU003]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRKGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDVIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELICPFCGETCQLYCKLNRPFRRTNVFELLFGKELGECMKEAKGEY
AHSSYETLQKLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1066516 ACH2FV_RS11425 WP_076840973.1 2232552..2233622(-) (comGA) [Bacillus safensis subsp. safensis strain KGU003]
TTGTATGGAATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCAGTGTCATTTCGTGTTGATTCAGATTTAATCCAGCAGCGAACGATTGATAAAAAAAGCGGAG
AGAGGCTTATTGCACATTTTAAGTTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGGCCGCAAAATGGCTCACTAGCT
GTGATGCTAAGAAAAGGTCAAGTATTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGCTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGATTATTATCCTTTTTAA
ACCACTCGCATGGACTCATTTTATTTACTGGGCCAACAAATTCAGGGAAAACGACAACACTATATTCATTGATCCAGTTT
GCAAAAAAGAATTTTAACCGAAATATTATTACCCTTGAAGATCCTGTCGAAACAAGGAATGAAGAAGTGCTGCAGGTTCA
AGTGAATGAAAAAGCCGGCATCACGTATGCCGCTGGATTACGCGCTATTTTAAGACATGATCCAGATGTGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCGAGAACGGCGATTAGAGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAATGCAAAAGGAGCCCTTTACCGCATGCTTGAATTTGGTGTCACGATGAATGAACTCGAACAAACCATGGTTGC
GATTGCTGCCCAGCGATTAATTGAGCTCATATGTCCTTTTTGCGGAGAAACATGTCAGCTTTACTGTAAATTAAATCGGC
CTTTCAGACGAACAAATGTATTTGAATTGCTTTTCGGAAAAGAGCTTGGTGAGTGTATGAAAGAGGCAAAAGGAGAATAT
GCTCATTCGTCATATGAAACATTGCAAAAATTAATTCGTAAAGGAGTGGCACTAGGCTATTTATCGAAAAACACATATCA
CCGCTGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.407

99.438

0.64

Multiple sequence alignment