Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   ACHWYH_RS06055 Genome accession   NZ_CP172084
Coordinates   1215903..1216442 (+) Length   179 a.a.
NCBI ID   WP_005654843.1    Uniprot ID   A0A0H3PFD4
Organism   Haemophilus influenzae strain GA81666     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1210903..1221442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHWYH_RS06035 - 1211360..1212280 (-) 921 WP_005654835.1 ATP-binding cassette domain-containing protein -
  ACHWYH_RS06040 - 1212280..1213161 (-) 882 WP_005662662.1 metal ABC transporter substrate-binding protein -
  ACHWYH_RS06045 - 1213526..1214404 (-) 879 WP_005656360.1 D-alanyl-D-alanine carboxypeptidase family protein -
  ACHWYH_RS06050 - 1214651..1215802 (+) 1152 WP_005669104.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ACHWYH_RS06055 pilF/pilF2 1215903..1216442 (+) 540 WP_005654843.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACHWYH_RS06060 - 1216514..1217425 (+) 912 WP_005669105.1 RodZ family helix-turn-helix domain-containing protein -
  ACHWYH_RS06065 ispG 1217434..1218540 (+) 1107 WP_032823939.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACHWYH_RS06070 hisS 1218550..1219821 (+) 1272 WP_032823940.1 histidine--tRNA ligase -
  ACHWYH_RS06075 - 1219839..1220453 (+) 615 WP_032823941.1 YfgM family protein -
  ACHWYH_RS06080 iscX 1220504..1220698 (-) 195 WP_005626949.1 Fe-S cluster assembly protein IscX -
  ACHWYH_RS06085 fdx 1220698..1221039 (-) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20586.20 Da        Isoelectric Point: 8.2626

>NTDB_id=1065053 ACHWYH_RS06055 WP_005654843.1 1215903..1216442(+) (pilF/pilF2) [Haemophilus influenzae strain GA81666]
MKTISKQLSAVIFPFIFSACVSQSASNLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGEIENAHREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=1065053 ACHWYH_RS06055 WP_005654843.1 1215903..1216442(+) (pilF/pilF2) [Haemophilus influenzae strain GA81666]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTTCCTTTTATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAA
CTTAAATCATCAAGCTGCCGCTAAAGCTCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGGAAATAGAGAATGCACATCGTGAATATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAGCAACAATTTGAATTAGCACTTAATTCGCCAAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3PFD4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

97.765

100

0.978
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment