Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   ACHWYG_RS05780 Genome accession   NZ_CP172082
Coordinates   1177943..1178482 (+) Length   179 a.a.
NCBI ID   WP_112081654.1    Uniprot ID   -
Organism   Haemophilus influenzae strain GA54827     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1172943..1183482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHWYG_RS05760 - 1173590..1174510 (-) 921 WP_005654835.1 ATP-binding cassette domain-containing protein -
  ACHWYG_RS05765 - 1174510..1175391 (-) 882 WP_032823936.1 metal ABC transporter substrate-binding protein -
  ACHWYG_RS05770 - 1175568..1176446 (-) 879 WP_005649130.1 D-alanyl-D-alanine carboxypeptidase family protein -
  ACHWYG_RS05775 - 1176691..1177842 (+) 1152 WP_181913439.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ACHWYG_RS05780 pilF/pilF2 1177943..1178482 (+) 540 WP_112081654.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACHWYG_RS05785 - 1178554..1179465 (+) 912 WP_032823938.1 RodZ family helix-turn-helix domain-containing protein -
  ACHWYG_RS05790 ispG 1179474..1180580 (+) 1107 WP_032823939.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACHWYG_RS05795 hisS 1180590..1181861 (+) 1272 WP_032823940.1 histidine--tRNA ligase -
  ACHWYG_RS05800 - 1181879..1182493 (+) 615 WP_032823941.1 YfgM family protein -
  ACHWYG_RS05805 iscX 1182544..1182738 (-) 195 WP_005626949.1 Fe-S cluster assembly protein IscX -
  ACHWYG_RS05810 fdx 1182738..1183079 (-) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20653.37 Da        Isoelectric Point: 9.2106

>NTDB_id=1065017 ACHWYG_RS05780 WP_112081654.1 1177943..1178482(+) (pilF/pilF2) [Haemophilus influenzae strain GA54827]
MQTISKQLSAVIFPFIFSACVSQSASSLDHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIALCAYSAKKMNIYQQT
LEKLRQIDRKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=1065017 ACHWYG_RS05780 WP_112081654.1 1177943..1178482(+) (pilF/pilF2) [Haemophilus influenzae strain GA54827]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCAGCATCTAG
CTTAGATCATCAAGCTGCCGCCAAAGCGCGAGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCTCAGCAACAATTTGAATTAGCCCTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGAATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATAGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(73-98)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

95.531

100

0.955
  pilF Glaesserella parasuis strain SC1401

45.81

100

0.458

Multiple sequence alignment