Detailed information NTG1062242

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACF8J0_RS10900
• Genome accession: NZ_CP171591
• Coordinates: 2236002..2236802 (-)
• Length: 266 a.a.
• NCBI ID: WP_004191960.1
• Uniprot ID: A0AAJ5B1F9
• Organism: Klebsiella pneumoniae strain KP03_UID2108280003
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2231002..2241802

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACF8J0_RS10880	dksA	2231582..2232037 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
ACF8J0_RS10885	sfsA	2232200..2232916 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
ACF8J0_RS10890	thpR	2232916..2233452 (-)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
ACF8J0_RS10895	hrpB	2233525..2235954 (+)	2430	WP_032433845.1	ATP-dependent helicase HrpB	-
ACF8J0_RS10900	pilD	2236002..2236802 (-)	801	WP_004191960.1	A24 family peptidase	Machinery gene
ACF8J0_RS10905	-	2236799..2237545 (-)	747	WP_004145897.1	type II secretion system protein N	-
ACF8J0_RS10910	-	2237535..2238020 (-)	486	WP_004145896.1	type II secretion system protein M	-
ACF8J0_RS10915	gspL	2238013..2239206 (-)	1194	WP_032416685.1	type II secretion system protein GspL	-
ACF8J0_RS10920	gspK	2239193..2240179 (-)	987	WP_077255453.1	type II secretion system minor pseudopilin GspK	-
ACF8J0_RS10925	gspJ	2240176..2240772 (-)	597	WP_032433846.1	type II secretion system minor pseudopilin GspJ	-
ACF8J0_RS10930	gspI	2240769..2241134 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
ACF8J0_RS10935	gspH	2241131..2241646 (-)	516	WP_032422016.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29403.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=1062242 ACF8J0_RS10900 WP_004191960.1 2236002..2236802(-) (pilD) [Klebsiella pneumoniae strain KP03_UID2108280003]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=1062242 ACF8J0_RS10900 WP_004191960.1 2236002..2236802(-) (pilD) [Klebsiella pneumoniae strain KP03_UID2108280003]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421