Detailed information NTG1062174

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACF8KH_RS12955
• Genome accession: NZ_CP171580
• Coordinates: 2704015..2704815 (-)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain KP06_UID2108280006
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2699015..2709815

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACF8KH_RS12935	dksA	2699595..2700050 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
ACF8KH_RS12940	sfsA	2700213..2700929 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
ACF8KH_RS12945	thpR	2700929..2701465 (-)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
ACF8KH_RS12950	hrpB	2701538..2703967 (+)	2430	WP_058229143.1	ATP-dependent helicase HrpB	-
ACF8KH_RS12955	pilD	2704015..2704815 (-)	801	WP_002888947.1	A24 family peptidase	Machinery gene
ACF8KH_RS12960	-	2704812..2705558 (-)	747	WP_395164796.1	type II secretion system protein N	-
ACF8KH_RS12965	-	2705548..2706033 (-)	486	WP_004145896.1	type II secretion system protein M	-
ACF8KH_RS12970	gspL	2706026..2707219 (-)	1194	WP_101991231.1	type II secretion system protein GspL	-
ACF8KH_RS12975	gspK	2707206..2708192 (-)	987	WP_077258272.1	type II secretion system minor pseudopilin GspK	-
ACF8KH_RS12980	gspJ	2708189..2708785 (-)	597	WP_032411156.1	type II secretion system minor pseudopilin GspJ	-
ACF8KH_RS12985	gspI	2708782..2709147 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
ACF8KH_RS12990	gspH	2709144..2709659 (-)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=1062174 ACF8KH_RS12955 WP_002888947.1 2704015..2704815(-) (pilD) [Klebsiella pneumoniae strain KP06_UID2108280006]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=1062174 ACF8KH_RS12955 WP_002888947.1 2704015..2704815(-) (pilD) [Klebsiella pneumoniae strain KP06_UID2108280006]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGGCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425