Detailed information NTG1061705

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACF8KI_RS25645
• Genome accession: NZ_CP171505
• Coordinates: 5228610..5229410 (-)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain KP32_UID2108280028
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5223610..5234410

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACF8KI_RS25625	dksA	5224190..5224645 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
ACF8KI_RS25630	sfsA	5224808..5225524 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
ACF8KI_RS25635	thpR	5225524..5226060 (-)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
ACF8KI_RS25640	hrpB	5226133..5228562 (+)	2430	WP_029602503.1	ATP-dependent helicase HrpB	-
ACF8KI_RS25645	pilD	5228610..5229410 (-)	801	WP_004145898.1	A24 family peptidase	Machinery gene
ACF8KI_RS25650	-	5229407..5230153 (-)	747	WP_023316460.1	type II secretion system protein N	-
ACF8KI_RS25655	-	5230143..5230628 (-)	486	WP_004145896.1	type II secretion system protein M	-
ACF8KI_RS25660	gspL	5230621..5231814 (-)	1194	WP_029602501.1	type II secretion system protein GspL	-
ACF8KI_RS25665	gspK	5231801..5232787 (-)	987	WP_004178610.1	type II secretion system minor pseudopilin GspK	-
ACF8KI_RS25670	gspJ	5232784..5233380 (-)	597	WP_012737414.1	type II secretion system minor pseudopilin GspJ	-
ACF8KI_RS25675	gspI	5233377..5233742 (-)	366	WP_029602500.1	type II secretion system minor pseudopilin GspI	-
ACF8KI_RS25680	gspH	5233739..5234254 (-)	516	WP_029602499.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=1061705 ACF8KI_RS25645 WP_004145898.1 5228610..5229410(-) (pilD) [Klebsiella pneumoniae strain KP32_UID2108280028]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=1061705 ACF8KI_RS25645 WP_004145898.1 5228610..5229410(-) (pilD) [Klebsiella pneumoniae strain KP32_UID2108280028]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425