Detailed information NTG1061639

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACF8JU_RS03900
• Genome accession: NZ_CP171499
• Coordinates: 824960..825760 (-)
• Length: 266 a.a.
• NCBI ID: WP_395165426.1
• Uniprot ID: -
• Organism: Klebsiella variicola strain KV36_UID2108280032
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 819960..830760

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACF8JU_RS03880	dksA	820541..820996 (-)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-
ACF8JU_RS03885	sfsA	821159..821875 (-)	717	WP_022066362.1	DNA/RNA nuclease SfsA	-
ACF8JU_RS03890	thpR	821875..822411 (-)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
ACF8JU_RS03895	hrpB	822484..824913 (+)	2430	WP_032732528.1	ATP-dependent helicase HrpB	-
ACF8JU_RS03900	pilD	824960..825760 (-)	801	WP_395165426.1	prepilin peptidase	Machinery gene
ACF8JU_RS03905	-	825757..826503 (-)	747	WP_070612007.1	type II secretion system protein N	-
ACF8JU_RS03910	-	826493..826978 (-)	486	WP_012968921.1	type II secretion system protein M	-
ACF8JU_RS03915	gspL	826971..828167 (-)	1197	WP_039102856.1	type II secretion system protein GspL	-
ACF8JU_RS03920	gspK	828160..829140 (-)	981	WP_012542824.1	type II secretion system minor pseudopilin GspK	-
ACF8JU_RS03925	gspJ	829137..829733 (-)	597	WP_032731630.1	type II secretion system minor pseudopilin GspJ	-
ACF8JU_RS03930	gspI	829730..830095 (-)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
ACF8JU_RS03935	gspH	830092..830607 (-)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29471.23 Da
• Isoelectric Point: 8.7151

>NTDB_id=1061639 ACF8JU_RS03900 WP_395165426.1 824960..825760(-) (pilD) [Klebsiella variicola strain KV36_UID2108280032]
MTILTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLPFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=1061639 ACF8JU_RS03900 WP_395165426.1 824960..825760(-) (pilD) [Klebsiella variicola strain KV36_UID2108280032]
ATGACGATATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGCCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.811	99.624	0.496
pilD	Vibrio campbellii strain DS40M4	45.818	100	0.474
pilD	Acinetobacter nosocomialis M2	45.247	98.872	0.447
pilD	Acinetobacter baumannii D1279779	44.487	98.872	0.44
pilD	Neisseria gonorrhoeae MS11	42.912	98.12	0.421