Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   ACF6ZU_RS08500 Genome accession   NZ_CP171224
Coordinates   1832357..1833946 (+) Length   529 a.a.
NCBI ID   WP_392891520.1    Uniprot ID   -
Organism   Pseudomonas migulae strain HY-2     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 1827357..1838946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF6ZU_RS08480 (ACF6ZU_08480) pgeF 1829150..1829875 (-) 726 WP_392891514.1 peptidoglycan editing factor PgeF -
  ACF6ZU_RS08485 (ACF6ZU_08485) rluD 1829872..1830834 (-) 963 WP_008028788.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  ACF6ZU_RS08490 (ACF6ZU_08490) - 1830986..1832008 (+) 1023 WP_392891517.1 outer membrane protein assembly factor BamD -
  ACF6ZU_RS08495 (ACF6ZU_08495) - 1832134..1832367 (+) 234 WP_392891518.1 PP0621 family protein -
  ACF6ZU_RS08500 (ACF6ZU_08500) pilS 1832357..1833946 (+) 1590 WP_392891520.1 ATP-binding protein Regulator
  ACF6ZU_RS08505 (ACF6ZU_08505) pilR 1833949..1835280 (+) 1332 WP_392891522.1 sigma-54-dependent transcriptional regulator Regulator
  ACF6ZU_RS08510 (ACF6ZU_08510) thiO 1835400..1836500 (-) 1101 WP_392891524.1 glycine oxidase ThiO -
  ACF6ZU_RS08515 (ACF6ZU_08515) - 1836558..1836959 (-) 402 WP_392891526.1 type IV pilin protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58382.07 Da        Isoelectric Point: 6.3876

>NTDB_id=1060536 ACF6ZU_RS08500 WP_392891520.1 1832357..1833946(+) (pilS) [Pseudomonas migulae strain HY-2]
MIAETSSPGSKQAQRLLRLYHLYRLSVGITLVLLISSNLDNQLLTFTNDNLLRSGSWLYLVLNILLVVFLENTRRPAQLF
SLALIDVLLLCSLFYAAGGVASAIGNLLVVSVAISSALLRRRIGLLIAAVGALGIVGLSFLLSFSHPASPNDYLQVGTLG
ALCFAAALLVQGLMQRVEVSETLAEQRAGEVVGLEALNALILQRMRTGILVLDDQRQVQLANHSALTLLGRDTLEGERID
DCSPTLVERLQLWLNNPTLRPQSLKVSSSGLELQPSFIALDQSPHPQTLVFLEDLAQIAQQAQQLKLAALGRLTAGIAHE
IRNPLGAISHAAQLLQESEELNGADRRLTQIIQDHSQRMNRVIENVLQLSRRQQTLAQRLDLKPWLEQFVADSREGALDH
QQIHLRIGSGDFTTLMDPNQLTQVLDNLLRNAWRHSAQRHDTAQVWLDLFVDPDTQLPIIEVLDDGAGVAPDQQVHLFEP
FFTTSAQGTGLGLYLSRELCESNQARLDFKPRHDGGCFRITFAHGRKQA

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=1060536 ACF6ZU_RS08500 WP_392891520.1 1832357..1833946(+) (pilS) [Pseudomonas migulae strain HY-2]
GTGATCGCTGAGACGTCCAGTCCCGGCAGCAAACAGGCTCAGCGACTGCTGCGCCTTTATCACCTCTACCGTTTGAGTGT
CGGCATCACTCTGGTGCTGTTGATCTCCAGCAACCTCGACAACCAGTTGCTGACGTTCACCAATGACAACCTGCTGCGCA
GTGGCAGCTGGTTGTACCTGGTGCTGAATATTCTGTTGGTGGTCTTCCTTGAGAACACCCGGCGCCCGGCACAACTGTTC
AGCCTGGCGCTGATCGATGTCCTGCTCCTGTGCAGTCTTTTCTATGCCGCCGGCGGTGTCGCCAGCGCCATCGGCAACCT
GCTGGTGGTCTCGGTGGCGATCAGCAGCGCCTTGCTGCGAAGACGAATCGGCTTGTTGATTGCCGCCGTCGGCGCGCTCG
GCATCGTCGGATTGAGCTTTCTCCTCAGCTTCAGCCATCCCGCCAGTCCTAACGATTACCTGCAAGTCGGTACGCTCGGG
GCGCTGTGCTTTGCGGCGGCCTTGCTGGTGCAAGGCCTGATGCAGCGCGTGGAGGTCAGCGAAACGCTCGCCGAGCAACG
GGCCGGTGAAGTCGTAGGCCTCGAAGCCCTCAATGCGCTGATCCTGCAACGCATGCGCACCGGCATCCTGGTGCTGGACG
ACCAGCGGCAAGTGCAACTGGCCAACCACAGCGCGTTGACGCTGTTGGGCCGGGACACGCTTGAAGGTGAGCGGATCGAT
GATTGTTCACCGACGCTGGTCGAACGTCTGCAGCTGTGGCTGAACAACCCGACGTTGCGCCCCCAGAGCCTGAAAGTCAG
CAGCAGCGGCCTGGAACTGCAACCAAGCTTCATTGCCCTGGACCAGAGCCCGCACCCACAAACCCTGGTGTTTCTCGAGG
ATCTCGCGCAAATCGCCCAGCAGGCGCAACAACTGAAACTCGCCGCCCTGGGCCGCCTGACCGCAGGCATCGCCCACGAA
ATTCGCAATCCTTTGGGCGCCATCAGCCACGCTGCGCAGCTATTGCAGGAGTCGGAGGAACTGAACGGCGCGGATCGGCG
TCTGACCCAGATTATTCAAGATCACTCTCAGCGCATGAATCGGGTTATCGAAAACGTCCTGCAACTGTCCCGCCGCCAGC
AGACCCTGGCGCAACGCCTCGATTTGAAGCCATGGCTGGAGCAATTCGTTGCCGACAGTCGTGAAGGCGCGCTCGATCAC
CAGCAGATTCACCTGCGCATCGGTTCAGGCGACTTCACCACCCTGATGGACCCGAACCAGCTGACCCAGGTGCTCGACAA
TCTGCTGCGCAATGCTTGGCGCCACAGCGCTCAACGCCACGACACGGCACAGGTCTGGCTTGATCTGTTCGTCGACCCTG
ACACTCAGCTGCCGATCATCGAAGTGCTGGACGACGGCGCCGGTGTGGCGCCGGATCAGCAGGTGCACCTGTTCGAACCC
TTTTTCACCACCAGCGCCCAAGGCACGGGACTGGGGCTTTATCTGTCCCGTGAACTGTGCGAAAGCAATCAGGCGCGCCT
AGACTTCAAACCACGCCACGATGGCGGCTGCTTTCGCATCACCTTTGCTCACGGACGGAAACAAGCTTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-522)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

60.302

100

0.603

Multiple sequence alignment