Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   ACF0HW_RS01805 Genome accession   NZ_CP171208
Coordinates   349586..350971 (+) Length   461 a.a.
NCBI ID   WP_012118573.1    Uniprot ID   A7Z9B6
Organism   Bacillus amyloliquefaciens strain HN11     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 344586..355971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF0HW_RS01785 - 345733..346380 (-) 648 WP_007614107.1 YigZ family protein -
  ACF0HW_RS01790 degS 346603..347766 (+) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  ACF0HW_RS01795 degU 347843..348532 (+) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  ACF0HW_RS01800 - 348630..349478 (+) 849 WP_003151387.1 DegV family protein -
  ACF0HW_RS01805 comFA 349586..350971 (+) 1386 WP_012118573.1 DEAD/DEAH box helicase Machinery gene
  ACF0HW_RS01810 - 351029..351313 (+) 285 WP_007407481.1 late competence development ComFB family protein -
  ACF0HW_RS01815 comFC 351319..352008 (+) 690 WP_197197403.1 phosphoribosyltransferase family protein Machinery gene
  ACF0HW_RS01820 - 352082..352501 (+) 420 WP_012118571.1 TIGR03826 family flagellar region protein -
  ACF0HW_RS01825 flgM 352571..352837 (+) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  ACF0HW_RS01830 - 352852..353334 (+) 483 WP_003151394.1 flagellar protein FlgN -
  ACF0HW_RS01835 flgK 353350..354867 (+) 1518 WP_012118570.1 flagellar hook-associated protein FlgK -
  ACF0HW_RS01840 flgL 354879..355796 (+) 918 WP_012118569.1 flagellar hook-associated protein FlgL -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52493.84 Da        Isoelectric Point: 10.2968

>NTDB_id=1060454 ACF0HW_RS01805 WP_012118573.1 349586..350971(+) (comFA) [Bacillus amyloliquefaciens strain HN11]
MSEGASEFSEVRDFFFGRHLLRSEVPFSDHHIKRVTEKEYITAEPSIIRRKNRYVCQRCGQSDQASFAAFWAPSAKRQIT
YCRACVVMGRADELTSLYSWNQAHENSWEPVKLSWEGTLTDGQKRAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQSHLPVFLFVPSVHVLKKTTDYFQKLNIRAEGVHAEDTFRKDKVKRFRNGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGITSGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=1060454 ACF0HW_RS01805 WP_012118573.1 349586..350971(+) (comFA) [Bacillus amyloliquefaciens strain HN11]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTTCGGCCGGCATTTATTGAGATCTGAGGTACCGTT
TTCAGATCATCACATCAAGCGTGTTACAGAAAAAGAATATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCAGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCGTGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCCATGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCGGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCGCTGAATCACGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGTCTGACTCCGTTAATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGCCAAAAACTTCAATCACATCTGCCGGTTTTCTTATTCGTTCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGGTTCAGAAACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTCACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATCACAAGCGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(118-266)

Helicase conserved C-terminal domain (Helicase_C)

(330-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7Z9B6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.174

99.783

0.62

Multiple sequence alignment