Detailed information NTG1060415

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACF836_RS25745
• Genome accession: NZ_CP171165
• Coordinates: 5119080..5119880 (-)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain KP2010
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5114080..5124880

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACF836_RS25725 (ACF836_25720)	dksA	5114660..5115115 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
ACF836_RS25730 (ACF836_25725)	sfsA	5115278..5115994 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
ACF836_RS25735 (ACF836_25730)	thpR	5115994..5116530 (-)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
ACF836_RS25740 (ACF836_25735)	hrpB	5116603..5119032 (+)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
ACF836_RS25745 (ACF836_25740)	pilD	5119080..5119880 (-)	801	WP_002888947.1	A24 family peptidase	Machinery gene
ACF836_RS25750 (ACF836_25745)	-	5119877..5120623 (-)	747	WP_004145897.1	type II secretion system protein N	-
ACF836_RS25755 (ACF836_25750)	-	5120613..5121098 (-)	486	WP_004151943.1	type II secretion system protein M	-
ACF836_RS25760 (ACF836_25755)	gspL	5121091..5122284 (-)	1194	WP_004151942.1	type II secretion system protein GspL	-
ACF836_RS25765 (ACF836_25760)	gspK	5122271..5123257 (-)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
ACF836_RS25770 (ACF836_25765)	gspJ	5123254..5123850 (-)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
ACF836_RS25775 (ACF836_25770)	gspI	5123847..5124212 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
ACF836_RS25780 (ACF836_25775)	gspH	5124209..5124724 (-)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=1060415 ACF836_RS25745 WP_002888947.1 5119080..5119880(-) (pilD) [Klebsiella pneumoniae strain KP2010]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=1060415 ACF836_RS25745 WP_002888947.1 5119080..5119880(-) (pilD) [Klebsiella pneumoniae strain KP2010]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425