Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   ACF8MK_RS09305 Genome accession   NZ_CP171154
Coordinates   2159180..2161510 (-) Length   776 a.a.
NCBI ID   WP_392536410.1    Uniprot ID   -
Organism   Legionella sp. 227     
Function   ssDNA transport through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2154180..2166510
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF8MK_RS09275 (ACF8MK_09275) - 2154446..2154895 (-) 450 WP_265704471.1 Rrf2 family transcriptional regulator -
  ACF8MK_RS09280 (ACF8MK_09280) - 2155048..2155380 (+) 333 WP_392536407.1 hypothetical protein -
  ACF8MK_RS09285 (ACF8MK_09285) - 2155666..2156481 (+) 816 WP_265704473.1 mechanosensitive ion channel family protein -
  ACF8MK_RS09290 (ACF8MK_09290) - 2156773..2157000 (+) 228 WP_265704474.1 hypothetical protein -
  ACF8MK_RS09295 (ACF8MK_09295) - 2157161..2157799 (+) 639 WP_392536408.1 hypothetical protein -
  ACF8MK_RS09300 (ACF8MK_09300) - 2157914..2158963 (-) 1050 WP_392536409.1 bifunctional transcriptional activator/DNA repair enzyme AdaA -
  ACF8MK_RS09305 (ACF8MK_09305) comEC 2159180..2161510 (-) 2331 WP_392536410.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACF8MK_RS09310 (ACF8MK_09310) pilE 2161659..2162081 (-) 423 WP_392536411.1 type IV pilin protein Machinery gene
  ACF8MK_RS09315 (ACF8MK_09315) - 2162092..2165571 (-) 3480 WP_392536412.1 pilus assembly protein -
  ACF8MK_RS09320 (ACF8MK_09320) - 2165584..2166111 (-) 528 WP_392538946.1 pilus assembly protein PilX -

Sequence

Protein


Download         Length: 776 a.a.        Molecular weight: 87973.60 Da        Isoelectric Point: 9.5564

>NTDB_id=1060336 ACF8MK_RS09305 WP_392536410.1 2159180..2161510(-) (comEC) [Legionella sp. 227]
MEIFCFFLGILYLYTSNYFLPLINLLIFYLSPKYSIIGFFILGIVFAWLHQSLALPKGIATINPLSKHGTGVHASVHEDL
SILPMTQWFSAVAFQKRSIEVIPKVTIEGTIASIPTQDFTKTQFLLALEQYDHLPAQGLIQLSWYKNAPKLHSGQRWRFT
VKLKRPRNYLNPGSMDYVSSLAARHIFWTGYILPQENKLLTESPSNFSWLRLREYLSTQLTQLAPDQSTAGVVEALTLNL
TSHISQENWDLFRRTGTTHLFGISGEHIALISGMIYWLVRWLWRKSARCCLLIPAPYVACIMGLLAAVCYAFLAGFAPPV
QRALIGCFFYTLYRLGKLRFSTWQVWRYALFGVLCIEPHAVFMQGFYFSFIAVACLLLTQQRWQLKGYKGNLAIQLSCLI
GLMPLTLYWYAYGSINGFIANLFAIPLVGLLIVPLALITMTFCSWSFAGLLMKLLALLIALLFKGLNLIEHLASININGS
IQGMEWVIILMGVLLMWVLLPIKPFQWIAPLWVLLAVFPHQTLRSPGEAVIDVLDVGQGLAVVVRTQNHVLIYDTGDRFF
QGNDLGKMVILPYLKTLRVNKIDAVVISHPDKDHRGGLNSLEKGVLVAQRLVNEPFYYNNHGVRCHDYPPWRWDNIYFRF
LPITTHFKNKNNNSCILQISTSAGKVLLTGDIEKIAEDYLIRTYGGELASDVLIVPHHGSKTSSSYRFLLEVAPRYAIAS
LGFDNRFHFPHAKTLARMKSLNIPFYRTDHCGMTQITLPVQGEIKSPICFSGLKKV

Nucleotide


Download         Length: 2331 bp        

>NTDB_id=1060336 ACF8MK_RS09305 WP_392536410.1 2159180..2161510(-) (comEC) [Legionella sp. 227]
ATGGAAATTTTTTGCTTTTTTCTAGGCATTCTTTATCTATACACCTCAAATTACTTCTTACCCCTAATCAACTTACTGAT
TTTTTACCTCAGTCCCAAATATTCAATAATTGGTTTTTTTATTTTAGGGATCGTGTTCGCTTGGCTGCACCAATCTTTGG
CTTTACCCAAAGGAATAGCAACTATTAATCCTCTTTCGAAGCACGGGACCGGAGTGCACGCATCAGTACATGAAGATTTG
AGCATCCTGCCAATGACGCAGTGGTTCTCTGCAGTAGCCTTTCAAAAGAGATCTATTGAAGTAATACCCAAGGTCACAAT
AGAAGGGACCATTGCATCTATTCCTACCCAAGATTTTACCAAAACCCAATTTTTATTAGCTCTTGAACAATACGATCATC
TTCCTGCACAAGGTTTAATTCAATTATCCTGGTATAAAAATGCCCCCAAGTTACATTCAGGACAACGATGGCGATTCACA
GTCAAATTGAAAAGGCCACGCAATTATCTTAATCCTGGAAGCATGGATTACGTGAGCTCACTTGCAGCACGGCATATCTT
TTGGACCGGTTATATTCTTCCCCAAGAAAATAAACTGCTTACTGAATCACCTTCTAATTTTAGTTGGCTTCGATTACGTG
AATATTTAAGCACTCAACTCACCCAATTAGCCCCAGATCAATCCACAGCAGGTGTTGTTGAAGCCTTAACTTTAAATTTA
ACAAGCCATATTAGCCAAGAAAATTGGGATTTATTTAGGCGCACAGGCACAACTCATCTTTTTGGAATCTCTGGAGAGCA
TATTGCATTGATATCGGGGATGATTTATTGGCTGGTTCGCTGGTTATGGCGTAAAAGTGCGCGATGCTGCTTATTGATTC
CTGCCCCCTACGTAGCCTGCATTATGGGTCTCTTAGCTGCTGTATGTTATGCGTTTTTAGCGGGATTTGCCCCACCGGTA
CAAAGGGCATTAATCGGTTGTTTCTTTTATACGCTCTATCGCTTAGGAAAATTGCGCTTTTCTACCTGGCAAGTCTGGCG
TTATGCTTTGTTTGGGGTTTTATGTATTGAGCCTCATGCAGTATTCATGCAAGGATTTTATTTTTCCTTCATCGCAGTTG
CTTGTTTGCTTTTAACTCAACAACGCTGGCAATTAAAAGGTTATAAAGGAAATTTAGCCATACAATTAAGTTGTTTGATT
GGCCTCATGCCTTTAACCCTATACTGGTATGCTTATGGCTCCATAAATGGATTTATTGCGAACTTATTTGCTATTCCTTT
AGTTGGTCTACTGATTGTCCCCTTAGCATTAATCACCATGACATTCTGTTCCTGGAGTTTTGCTGGTTTATTGATGAAAC
TTTTGGCCTTACTGATTGCCTTATTATTTAAAGGATTAAATTTAATTGAGCATTTAGCAAGCATTAATATCAATGGATCC
ATTCAAGGTATGGAATGGGTTATTATTCTGATGGGAGTACTGTTGATGTGGGTTTTATTACCAATTAAGCCCTTTCAATG
GATTGCACCACTGTGGGTTCTACTCGCCGTTTTTCCACATCAAACGCTACGATCCCCAGGAGAAGCAGTGATTGATGTTT
TAGATGTTGGCCAAGGACTGGCAGTCGTCGTAAGGACTCAAAATCATGTTCTGATCTATGATACCGGAGATCGATTTTTC
CAAGGAAATGATTTAGGAAAAATGGTGATCTTACCTTATCTTAAAACTTTAAGAGTCAATAAAATTGATGCTGTAGTTAT
TAGCCATCCAGATAAAGATCACCGGGGCGGCCTTAACTCACTGGAGAAAGGGGTACTCGTAGCACAACGATTAGTAAATG
AGCCTTTTTATTATAATAATCATGGGGTGCGATGTCATGATTACCCGCCATGGAGATGGGATAACATATATTTTCGTTTT
TTGCCCATCACCACTCATTTTAAAAATAAAAATAACAATTCTTGTATTTTACAAATAAGTACATCGGCTGGAAAAGTACT
CTTAACCGGAGACATCGAAAAGATAGCCGAAGATTATTTAATAAGAACTTATGGAGGCGAACTCGCATCTGATGTTTTAA
TTGTTCCACATCATGGCAGTAAAACCTCCTCTTCTTATCGATTTTTACTCGAGGTTGCTCCCCGTTATGCAATAGCTTCT
TTAGGTTTTGACAATCGCTTTCATTTTCCACATGCTAAAACATTAGCACGGATGAAATCCTTGAATATTCCCTTTTATAG
AACGGACCATTGTGGCATGACACAAATCACCTTGCCTGTTCAAGGAGAAATTAAAAGCCCGATTTGTTTTAGTGGTCTTA
AAAAGGTGTAG

Domains

Predicted by InterproScan.

Metallo-beta-lactamase superfamily (Lactamase_B)

(535-720)

Competence protein (Competence)

(236-502)

Domain of unknown function (DUF4131) (DUF4131)

(102-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Legionella pneumophila strain Lp02

59.12

99.613

0.589
  comEC Legionella pneumophila strain ERS1305867

58.679

99.485

0.584

Multiple sequence alignment