Detailed information NTG1060227

Bioinformatically predicted

Overview

• Name: vraS
• Type: Regulator
• Locus tag: ACF3N8_RS06015
• Genome accession: NZ_CP171119
• Coordinates: 1255362..1256405 (+)
• Length: 347 a.a.
• NCBI ID: WP_009384036.1
• Uniprot ID: K9ALE2
• Organism: Staphylococcus massiliensis strain WGS1515
• Function: repress expression of competence genes (predicted from homology) Competence regulation

Genomic Context

Location: 1250362..1261405

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACF3N8_RS05990 (ACF3N8_05990)	-	1251282..1252004 (+)	723	WP_009384041.1	type 1 glutamine amidotransferase	-
ACF3N8_RS05995 (ACF3N8_05995)	-	1252187..1253215 (-)	1029	WP_009384040.1	aromatic acid exporter family protein	-
ACF3N8_RS06000 (ACF3N8_06000)	map	1253402..1254160 (+)	759	WP_009384039.1	type I methionyl aminopeptidase	-
ACF3N8_RS06005 (ACF3N8_06005)	-	1254263..1254649 (+)	387	WP_017176866.1	hypothetical protein	-
ACF3N8_RS06010 (ACF3N8_06010)	liaF	1254664..1255365 (+)	702	WP_238101028.1	cell wall-active antibiotics response protein LiaF	-
ACF3N8_RS06015 (ACF3N8_06015)	vraS	1255362..1256405 (+)	1044	WP_009384036.1	sensor histidine kinase	Regulator
ACF3N8_RS06020 (ACF3N8_06020)	vraR	1256395..1257024 (+)	630	WP_009384035.1	response regulator transcription factor	Regulator
ACF3N8_RS06025 (ACF3N8_06025)	-	1257690..1258262 (-)	573	WP_392449764.1	beta-class carbonic anhydrase	-
ACF3N8_RS06030 (ACF3N8_06030)	-	1258277..1259383 (-)	1107	WP_009384033.1	YihY/virulence factor BrkB family protein	-
ACF3N8_RS06035 (ACF3N8_06035)	-	1259500..1259799 (-)	300	WP_009384032.1	YtxH domain-containing protein	-
ACF3N8_RS06040 (ACF3N8_06040)	-	1259784..1260257 (-)	474	WP_009384031.1	low molecular weight protein-tyrosine-phosphatase	-
ACF3N8_RS06045 (ACF3N8_06045)	-	1260401..1260604 (+)	204	WP_238094244.1	DUF1128 family protein	-

Sequence

Protein

• Length: 347 a.a.
• Molecular weight: 40145.63 Da
• Isoelectric Point: 6.2472

>NTDB_id=1060227 ACF3N8_RS06015 WP_009384036.1 1255362..1256405(+) (vraS) [Staphylococcus massiliensis strain WGS1515]
MNHYVRSIGSLLILVYSVFIAFFFIDKVFANIIYFQGMFYQQIFGLPVFLFLNILVILLCIVFGSILAYKINQQNDWIKR
QIERAIEGETVGINDRQIELYHETIDLYKVLVPLNQELHKMRLKTQDLTNESYNLNDVKVKKIIEDERQRLARELHDSVS
QQLFAASLMLSAIKETKLEPPLDQQIVTLEKMVQDSQLEMRALLLHLRPIGLNNKSLGEGIKDLVTDLQKKVPMKVVKDI
EEFDVPKGIEDHLFRITQEAISNTLRHAKGTKVTIELLNREDYILLRIHDDGIGFNVDDKLEQSYGLKNMRERALEIGAT
FHIVSLPDSGTRIEVKAPFTKEENYAN

Nucleotide

• Length: 1044 bp

>NTDB_id=1060227 ACF3N8_RS06015 WP_009384036.1 1255362..1256405(+) (vraS) [Staphylococcus massiliensis strain WGS1515]
ATGAACCACTATGTGCGATCTATCGGAAGTCTCTTGATACTTGTTTATAGCGTTTTTATTGCTTTCTTTTTCATAGATAA
GGTATTTGCAAATATCATTTATTTCCAAGGGATGTTCTATCAACAAATCTTTGGACTTCCAGTATTTTTATTTCTAAATA
TCTTAGTAATACTACTATGCATTGTATTTGGTAGTATTTTAGCGTATAAAATCAATCAACAAAATGATTGGATTAAACGT
CAAATTGAACGCGCGATTGAAGGGGAAACGGTTGGAATTAATGATCGACAAATTGAACTTTATCATGAAACAATTGATTT
ATATAAAGTACTTGTTCCTCTAAATCAAGAGCTACACAAGATGCGTTTAAAGACGCAAGATTTAACGAATGAGTCTTATA
ACTTGAATGATGTTAAGGTTAAGAAAATAATAGAAGATGAAAGACAAAGACTTGCTAGAGAATTGCATGATTCTGTAAGT
CAGCAACTCTTCGCAGCAAGTTTAATGTTGTCTGCAATCAAAGAAACGAAACTCGAGCCACCATTAGACCAACAAATTGT
CACTTTAGAAAAGATGGTTCAAGATTCTCAACTTGAAATGCGTGCGCTACTTTTACACCTAAGACCAATTGGCTTAAATA
ATAAGTCACTAGGTGAAGGTATTAAAGACTTAGTAACGGACCTTCAGAAGAAAGTACCAATGAAAGTAGTGAAAGATATC
GAAGAATTTGATGTACCTAAAGGTATCGAAGACCACTTGTTCCGTATTACACAAGAGGCGATATCGAACACTTTACGTCA
TGCTAAAGGTACCAAAGTTACAATAGAGCTTTTAAATAGAGAAGACTATATTCTATTACGTATTCATGATGATGGAATTG
GATTTAATGTTGATGATAAACTCGAACAAAGTTATGGTTTAAAGAATATGCGAGAACGCGCGTTAGAAATCGGTGCAACA
TTCCACATCGTATCACTACCTGATTCTGGTACACGAATCGAAGTTAAAGCACCGTTTACCAAGGAGGAAAATTATGCCAA
TTAA

Domains

Predicted by InterproScan.

• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) (249-340)

• Histidine kinase (HisKA_3) (147-210)

3D structure

• AlphaFold DB: K9ALE2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
vraS	Staphylococcus aureus N315	83.478	99.424	0.83