Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGD   Type   Machinery gene
Locus tag   ACFW98_RS05795 Genome accession   NZ_CP170719
Coordinates   1284523..1284960 (-) Length   145 a.a.
NCBI ID   WP_012117983.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5008     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1279523..1289960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW98_RS05745 (ACFW98_05745) sinI 1279907..1280080 (+) 174 WP_003153105.1 anti-repressor SinI Regulator
  ACFW98_RS05750 (ACFW98_05750) sinR 1280114..1280449 (+) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  ACFW98_RS05755 (ACFW98_05755) tasA 1280497..1281282 (-) 786 WP_007408329.1 biofilm matrix protein TasA -
  ACFW98_RS05760 (ACFW98_05760) sipW 1281347..1281931 (-) 585 WP_015240205.1 signal peptidase I SipW -
  ACFW98_RS05765 (ACFW98_05765) tapA 1281903..1282574 (-) 672 WP_113766732.1 amyloid fiber anchoring/assembly protein TapA -
  ACFW98_RS05770 (ACFW98_05770) - 1282833..1283162 (+) 330 WP_095352945.1 DUF3889 domain-containing protein -
  ACFW98_RS05775 (ACFW98_05775) - 1283202..1283381 (-) 180 WP_003153093.1 YqzE family protein -
  ACFW98_RS05780 (ACFW98_05780) comGG 1283438..1283815 (-) 378 WP_015417814.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACFW98_RS05785 (ACFW98_05785) comGF 1283816..1284211 (-) 396 WP_064115389.1 competence type IV pilus minor pilin ComGF -
  ACFW98_RS05790 (ACFW98_05790) comGE 1284225..1284539 (-) 315 WP_064115388.1 competence type IV pilus minor pilin ComGE -
  ACFW98_RS05795 (ACFW98_05795) comGD 1284523..1284960 (-) 438 WP_012117983.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACFW98_RS05800 (ACFW98_05800) comGC 1284950..1285258 (-) 309 WP_015417818.1 competence type IV pilus major pilin ComGC Machinery gene
  ACFW98_RS05805 (ACFW98_05805) comGB 1285263..1286300 (-) 1038 WP_053573197.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACFW98_RS05810 (ACFW98_05810) comGA 1286287..1287357 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  ACFW98_RS05815 (ACFW98_05815) - 1287550..1288500 (-) 951 WP_015417820.1 magnesium transporter CorA family protein -
  ACFW98_RS05820 (ACFW98_05820) - 1288646..1289947 (+) 1302 WP_012117986.1 hemolysin family protein -

Sequence


Protein


Download         Length: 145 a.a.        Molecular weight: 16286.74 Da        Isoelectric Point: 10.2475

>NTDB_id=1058717 ACFW98_RS05795 WP_012117983.1 1284523..1284960(-) (comGD) [Bacillus amyloliquefaciens strain JP5008]
MNNNRRTENGFTLLESLIVLSLASVLLTVLFTTVPPAYTHLAVRQKTEQLQKDIQLAQETAIAEHKRTKITFLPKEHKYK
LQSGGRIVERSFDSLHITLVTLPESLEFNEKGHPNSGGKIQLKSAGFTYEITVYLGSGNVHAKRK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1058717 ACFW98_RS05795 WP_012117983.1 1284523..1284960(-) (comGD) [Bacillus amyloliquefaciens strain JP5008]
TTGAACAATAACAGGCGGACAGAAAATGGGTTTACCCTTCTTGAAAGCCTGATTGTGTTAAGTCTGGCGTCCGTCCTGCT
GACGGTTTTGTTCACGACGGTTCCGCCGGCCTACACCCACCTTGCCGTGCGGCAAAAAACAGAACAGCTCCAAAAAGATA
TTCAGCTTGCTCAGGAAACGGCTATCGCAGAACATAAAAGAACAAAAATCACTTTTCTTCCAAAAGAGCATAAATACAAA
CTGCAGTCAGGCGGAAGGATTGTCGAGCGTTCCTTTGATTCGCTTCACATTACACTTGTGACTTTACCGGAGAGCCTTGA
ATTTAACGAAAAAGGCCATCCGAATTCGGGAGGAAAGATCCAATTGAAGAGCGCCGGGTTCACCTATGAGATCACAGTTT
ACTTAGGGAGCGGAAATGTCCATGCTAAACGGAAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Bacillus subtilis subsp. subtilis str. 168

56.849

100

0.572


Multiple sequence alignment