Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   ACFXAA_RS14110 Genome accession   NZ_CP170717
Coordinates   2912200..2913585 (+) Length   461 a.a.
NCBI ID   WP_040238815.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5014     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2907200..2918585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFXAA_RS14090 (ACFXAA_14090) - 2908347..2908994 (-) 648 WP_007407485.1 YigZ family protein -
  ACFXAA_RS14095 (ACFXAA_14095) degS 2909217..2910380 (+) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  ACFXAA_RS14100 (ACFXAA_14100) degU 2910457..2911146 (+) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  ACFXAA_RS14105 (ACFXAA_14105) - 2911244..2912092 (+) 849 WP_015418336.1 DegV family protein -
  ACFXAA_RS14110 (ACFXAA_14110) comFA 2912200..2913585 (+) 1386 WP_040238815.1 DEAD/DEAH box helicase Machinery gene
  ACFXAA_RS14115 (ACFXAA_14115) - 2913643..2913927 (+) 285 WP_003151389.1 late competence development ComFB family protein -
  ACFXAA_RS14120 (ACFXAA_14120) comFC 2913933..2914622 (+) 690 WP_197093073.1 phosphoribosyltransferase family protein Machinery gene
  ACFXAA_RS14125 (ACFXAA_14125) - 2914696..2915115 (+) 420 WP_025285352.1 TIGR03826 family flagellar region protein -
  ACFXAA_RS14130 (ACFXAA_14130) flgM 2915185..2915451 (+) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  ACFXAA_RS14135 (ACFXAA_14135) - 2915466..2915948 (+) 483 WP_003151394.1 flagellar protein FlgN -
  ACFXAA_RS14140 (ACFXAA_14140) flgK 2915964..2917481 (+) 1518 WP_033575223.1 flagellar hook-associated protein FlgK -
  ACFXAA_RS14145 (ACFXAA_14145) flgL 2917493..2918410 (+) 918 WP_003151396.1 flagellar hook-associated protein FlgL -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52515.94 Da        Isoelectric Point: 10.0818

>NTDB_id=1058673 ACFXAA_RS14110 WP_040238815.1 2912200..2913585(+) (comFA) [Bacillus amyloliquefaciens strain JP5014]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRIKRFIEKEYITAEPSIIRRKNRYVCQRCGQSDQACFAAFWASSAKRQIT
YCRACVMMGRADELTSLYSWNQASENSWEPVKLSWEGTLTDGQKKAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
CQKLQSQLPVFLFVPSVDVLKKTTDYFQKLNIRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGMTNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=1058673 ACFXAA_RS14110 WP_040238815.1 2912200..2913585(+) (comFA) [Bacillus amyloliquefaciens strain JP5014]
ATGTCCGAGGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
TTCAGATCAGCGCATCAAGCGTTTTATAGAAAAAGAGTATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCTGCTTTGCGGCATTTTGGGCGTCCTCTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCTCTGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGAAGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTATGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCGCTGAATCACGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGTCTGACTCCGTTAATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTT
TGCCAAAAACTTCAATCACAGCTGCCTGTTTTCTTATTCGTTCCGTCTGTAGACGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGGTTCAGAGACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACCGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATGACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(118-266)

Helicase conserved C-terminal domain (Helicase_C)

(325-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

63.261

99.783

0.631

Multiple sequence alignment