Detailed information    

insolico Bioinformatically predicted

Overview

Name   phrF   Type   Regulator
Locus tag   ACFW99_RS18260 Genome accession   NZ_CP170651
Coordinates   3717663..3717782 (+) Length   39 a.a.
NCBI ID   WP_015240778.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5025     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context

Location: 3712663..3722782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW99_RS18240 (ACFW99_18240) - 3713013..3713525 (+) 513 Protein_3540 bacteriocin biosynthesis protein AlbG -
  ACFW99_RS18245 (ACFW99_18245) - 3713532..3714908 (-) 1377 WP_268556813.1 YncE family protein -
  ACFW99_RS18250 (ACFW99_18250) - 3714948..3716303 (-) 1356 WP_317859594.1 YncE family protein -
  ACFW99_RS18255 (ACFW99_18255) rapF 3716534..3717679 (+) 1146 WP_391590163.1 tetratricopeptide repeat protein Regulator
  ACFW99_RS18260 (ACFW99_18260) phrF 3717663..3717782 (+) 120 WP_015240778.1 PhrC/PhrF family phosphatase-inhibitory pheromone Regulator
  ACFW99_RS18265 (ACFW99_18265) - 3717980..3718183 (+) 204 WP_007407640.1 helix-turn-helix transcriptional regulator -
  ACFW99_RS18270 (ACFW99_18270) - 3718197..3718400 (+) 204 WP_007407641.1 hypothetical protein -
  ACFW99_RS18275 (ACFW99_18275) - 3718435..3718620 (+) 186 WP_007407642.1 DUF4177 domain-containing protein -
  ACFW99_RS18280 (ACFW99_18280) - 3718806..3719087 (+) 282 WP_045209208.1 hypothetical protein -
  ACFW99_RS18285 (ACFW99_18285) - 3719131..3719643 (+) 513 WP_031378560.1 stage II sporulation protein M -
  ACFW99_RS18290 (ACFW99_18290) - 3720234..3720452 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  ACFW99_RS18295 (ACFW99_18295) speB 3720620..3721492 (-) 873 WP_007407646.1 agmatinase -
  ACFW99_RS18300 (ACFW99_18300) speE 3721551..3722381 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 39 a.a.        Molecular weight: 4281.16 Da        Isoelectric Point: 10.0233

>NTDB_id=1058448 ACFW99_RS18260 WP_015240778.1 3717663..3717782(+) (phrF) [Bacillus amyloliquefaciens strain JP5025]
MKLKYKLALTCLAISTVFVSADIASAPKHQFEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=1058448 ACFW99_RS18260 WP_015240778.1 3717663..3717782(+) (phrF) [Bacillus amyloliquefaciens strain JP5025]
ATGAAATTGAAGTATAAATTAGCACTTACTTGCTTGGCAATAAGCACTGTTTTTGTTTCAGCCGACATCGCAAGTGCACC
GAAACATCAATTTGAAGTTGCTCAGCGCGGAATGATTTAA

Domains

Predicted by InterproScan.

Rap-phr extracellular signalling (PhrC_PhrF)

(3-39)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

74.074

69.231

0.513
  phrC Bacillus subtilis subsp. subtilis str. 168

46.154

100

0.462

Multiple sequence alignment