Detailed information    

insolico Bioinformatically predicted

Overview

Name   recN   Type   Machinery gene
Locus tag   ACFW99_RS12025 Genome accession   NZ_CP170651
Coordinates   2499300..2501030 (-) Length   576 a.a.
NCBI ID   WP_007408359.1    Uniprot ID   I2C7K7
Organism   Bacillus amyloliquefaciens strain JP5025     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2494300..2506030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW99_RS12000 (ACFW99_12000) - 2494719..2494931 (+) 213 WP_007408362.1 spore germination protein -
  ACFW99_RS12005 (ACFW99_12005) - 2495057..2495269 (+) 213 WP_003153181.1 spore germination protein -
  ACFW99_RS12010 (ACFW99_12010) alr 2495445..2496626 (+) 1182 WP_012117960.1 alanine racemase -
  ACFW99_RS12015 (ACFW99_12015) spo0A 2496775..2497575 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  ACFW99_RS12020 (ACFW99_12020) spoIVB 2497856..2499136 (-) 1281 WP_014418352.1 SpoIVB peptidase -
  ACFW99_RS12025 (ACFW99_12025) recN 2499300..2501030 (-) 1731 WP_007408359.1 DNA repair protein RecN Machinery gene
  ACFW99_RS12030 (ACFW99_12030) ahrC 2501062..2501511 (-) 450 WP_003153173.1 transcriptional regulator AhrC/ArgR -
  ACFW99_RS12035 (ACFW99_12035) - 2501608..2502453 (-) 846 WP_007612503.1 TlyA family RNA methyltransferase -
  ACFW99_RS12040 (ACFW99_12040) dxs 2502450..2504351 (-) 1902 WP_021494304.1 1-deoxy-D-xylulose-5-phosphate synthase -
  ACFW99_RS12045 (ACFW99_12045) - 2504495..2505385 (-) 891 WP_053284918.1 polyprenyl synthetase family protein -
  ACFW99_RS12050 (ACFW99_12050) - 2505375..2505629 (-) 255 WP_007408352.1 exodeoxyribonuclease VII small subunit -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 64079.91 Da        Isoelectric Point: 4.7628

>NTDB_id=1058414 ACFW99_RS12025 WP_007408359.1 2499300..2501030(-) (recN) [Bacillus amyloliquefaciens strain JP5025]
MLAELSIKNFAIIEELTISFERGLTVLTGETGAGKSIIIDAVSLLVGGRGSSEFVRYGETKAELEGLFLLDSGHPVFEVC
REQGIDASDDMIVMRRDINAGGKSVCRVNGKLVTIAALREIGRLLLDIHGQHDNQLLMEDDKHLELLDRFAGAEAESALQ
AYHEGYERYMKLLKKVKKLSESEQEMAHRLDLIQFQLEEIESAKLELNEDELLQEERKQISNFEKIYESLQNAYNALRSE
QGGLDWVGMASAQLEDISDINEPLQKLSESVSSSYYLLEDATFQMRNLLDDLEYDPERLNFIETRLNEIKQLKRKYGATV
EDILEYGSKIEEEIDQIENRDSHLEALKKELESVGKDVAVEAANLSKIRKAWAKKLAEAIHQELKSLYMGKSTFDTEFLV
KTDPSASEAPVVNGQPVQLTQKGIDLVKFLISTNTGEPLKPLSKVASGGELSRVMLAMKSIFSSQQDVTSIIFDEVDTGV
SGRVAQAIAEKIHKVSTGSQVLCITHLPQVAAMADTHLLIAKEFKDGRTTTHVTPLSKQDKVAEIGRMIAGVEVTDLTKR
HAQELLKQAGQVKTTG

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=1058414 ACFW99_RS12025 WP_007408359.1 2499300..2501030(-) (recN) [Bacillus amyloliquefaciens strain JP5025]
TTGTTAGCTGAACTATCAATAAAAAACTTTGCCATTATAGAGGAACTGACGATTTCGTTTGAGCGGGGGCTCACCGTCCT
GACGGGAGAAACAGGGGCCGGCAAGTCAATTATTATAGACGCCGTTTCCCTATTAGTCGGCGGCCGCGGCTCGTCTGAAT
TTGTCCGTTACGGTGAAACAAAGGCCGAGCTTGAAGGACTGTTTCTATTGGACAGCGGTCATCCCGTTTTTGAAGTATGC
CGTGAGCAGGGAATAGACGCATCTGACGACATGATTGTCATGAGAAGGGACATTAATGCCGGAGGAAAAAGCGTCTGCCG
TGTCAACGGAAAATTAGTGACGATTGCGGCTTTGAGGGAAATCGGCAGACTTTTATTAGATATTCACGGACAGCATGATA
ACCAGCTGTTAATGGAGGACGATAAACACTTAGAGCTGTTGGACAGGTTTGCGGGCGCTGAGGCCGAATCCGCGCTTCAG
GCTTACCATGAAGGCTATGAGCGTTATATGAAGCTGCTCAAAAAAGTGAAGAAGCTGTCAGAAAGCGAGCAGGAAATGGC
GCATCGGTTAGACCTGATTCAATTTCAGCTTGAAGAAATCGAGTCGGCGAAGCTTGAATTGAATGAAGACGAGCTTCTAC
AGGAAGAGCGGAAGCAGATATCCAACTTTGAAAAAATTTATGAATCATTGCAAAACGCCTATAATGCGCTCCGCAGCGAG
CAGGGGGGGCTTGACTGGGTCGGAATGGCCTCTGCCCAGCTTGAAGATATTTCGGATATTAATGAACCGCTGCAAAAGCT
GTCAGAAAGTGTGTCAAGTTCCTATTATCTGCTGGAGGATGCAACCTTTCAGATGCGCAATCTGTTAGATGATCTGGAAT
ATGACCCGGAACGGCTGAATTTTATTGAAACCCGTTTAAATGAGATCAAACAGCTGAAGCGCAAATACGGAGCGACGGTA
GAAGACATTTTGGAATACGGCTCCAAAATAGAAGAGGAAATCGATCAAATCGAGAACCGCGACAGCCACCTTGAAGCGCT
GAAGAAGGAATTGGAGTCGGTCGGCAAAGATGTGGCCGTAGAAGCCGCCAATTTATCAAAAATCAGAAAAGCTTGGGCTA
AGAAACTGGCGGAAGCGATTCATCAGGAATTAAAAAGTCTGTATATGGGCAAATCAACGTTTGATACGGAATTTCTCGTG
AAAACCGATCCGTCAGCCAGTGAAGCACCGGTTGTCAACGGGCAGCCCGTGCAGCTTACCCAAAAGGGGATTGACCTTGT
GAAGTTTTTAATTTCAACAAACACCGGTGAGCCTTTAAAACCGCTGTCAAAGGTCGCTTCCGGAGGCGAGCTTTCACGGG
TGATGCTGGCGATGAAGAGTATTTTTTCTTCCCAGCAGGATGTCACCTCCATCATCTTTGACGAAGTGGACACCGGTGTA
AGCGGGCGGGTTGCCCAAGCCATCGCGGAAAAAATCCATAAAGTATCGACCGGTTCACAGGTGCTCTGCATTACACACCT
GCCGCAGGTTGCCGCCATGGCGGATACGCACCTTCTGATCGCAAAAGAATTCAAAGACGGCCGGACGACAACGCATGTCA
CGCCGCTTTCCAAACAGGACAAGGTAGCGGAAATCGGCCGCATGATTGCGGGCGTTGAAGTGACCGATCTGACAAAACGG
CATGCACAAGAGCTTTTAAAACAGGCCGGACAGGTAAAAACGACAGGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_23)

(4-230)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recN Bacillus subtilis subsp. subtilis str. 168

88.715

100

0.887

Multiple sequence alignment