Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   ACFW99_RS12015 Genome accession   NZ_CP170651
Coordinates   2496775..2497575 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus amyloliquefaciens strain JP5025     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 2491775..2502575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW99_RS11985 (ACFW99_11985) - 2492315..2492947 (+) 633 WP_007408365.1 helix-turn-helix domain-containing protein -
  ACFW99_RS11990 (ACFW99_11990) - 2493244..2493381 (+) 138 WP_014470646.1 hypothetical protein -
  ACFW99_RS11995 (ACFW99_11995) - 2493475..2494593 (+) 1119 WP_032863662.1 NADH-dependent flavin oxidoreductase -
  ACFW99_RS12000 (ACFW99_12000) - 2494719..2494931 (+) 213 WP_007408362.1 spore germination protein -
  ACFW99_RS12005 (ACFW99_12005) - 2495057..2495269 (+) 213 WP_003153181.1 spore germination protein -
  ACFW99_RS12010 (ACFW99_12010) alr 2495445..2496626 (+) 1182 WP_012117960.1 alanine racemase -
  ACFW99_RS12015 (ACFW99_12015) spo0A 2496775..2497575 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  ACFW99_RS12020 (ACFW99_12020) spoIVB 2497856..2499136 (-) 1281 WP_014418352.1 SpoIVB peptidase -
  ACFW99_RS12025 (ACFW99_12025) recN 2499300..2501030 (-) 1731 WP_007408359.1 DNA repair protein RecN Machinery gene
  ACFW99_RS12030 (ACFW99_12030) ahrC 2501062..2501511 (-) 450 WP_003153173.1 transcriptional regulator AhrC/ArgR -
  ACFW99_RS12035 (ACFW99_12035) - 2501608..2502453 (-) 846 WP_007612503.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=1058413 ACFW99_RS12015 WP_003153177.1 2496775..2497575(-) (spo0A) [Bacillus amyloliquefaciens strain JP5025]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1058413 ACFW99_RS12015 WP_003153177.1 2496775..2497575(-) (spo0A) [Bacillus amyloliquefaciens strain JP5025]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCAGATGTGCTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATTCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCTGCGCAAAGCAGCATTATCCGCAGCCAGCCTGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGTATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAGCCAACCAACTCGGAATTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97

Multiple sequence alignment