Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACFW99_RS01660 Genome accession   NZ_CP170651
Coordinates   340625..341062 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5025     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 335625..346062
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW99_RS01635 (ACFW99_01635) - 336645..337781 (+) 1137 WP_015416779.1 zinc-dependent alcohol dehydrogenase -
  ACFW99_RS01640 (ACFW99_01640) - 337796..338230 (+) 435 WP_007409358.1 RDD family protein -
  ACFW99_RS01645 (ACFW99_01645) - 338302..338625 (+) 324 WP_060675310.1 YckD family protein -
  ACFW99_RS01650 (ACFW99_01650) - 338729..340165 (+) 1437 WP_060675311.1 glycoside hydrolase family 1 protein -
  ACFW99_RS01655 (ACFW99_01655) nin/comJ 340206..340604 (-) 399 WP_020955304.1 competence protein ComJ Regulator
  ACFW99_RS01660 (ACFW99_01660) nucA/comI 340625..341062 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACFW99_RS01665 (ACFW99_01665) hxlB 341420..341977 (-) 558 WP_039252823.1 6-phospho-3-hexuloisomerase -
  ACFW99_RS01670 (ACFW99_01670) hxlA 341974..342609 (-) 636 WP_031378686.1 3-hexulose-6-phosphate synthase -
  ACFW99_RS01675 (ACFW99_01675) - 342841..343203 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=1058382 ACFW99_RS01660 WP_003156588.1 340625..341062(-) (nucA/comI) [Bacillus amyloliquefaciens strain JP5025]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1058382 ACFW99_RS01660 WP_003156588.1 340625..341062(-) (nucA/comI) [Bacillus amyloliquefaciens strain JP5025]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCCCTTAAACATGTGCCTGTCAAAACGGGATACGATAGAGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment