Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   O1Q83_RS06855 Genome accession   NZ_CP170642
Coordinates   1389194..1389715 (-) Length   173 a.a.
NCBI ID   WP_132302241.1    Uniprot ID   A0A4R1KUL6
Organism   Lonepinella koalarum strain ACM3666     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1384194..1394715
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1Q83_RS06830 (O1Q83_01344) - 1384449..1384700 (+) 252 WP_132302246.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  O1Q83_RS06835 (O1Q83_01345) - 1384697..1385047 (+) 351 WP_132302245.1 type II toxin-antitoxin system PemK/MazF family toxin -
  O1Q83_RS06840 (O1Q83_01346) ligA 1385078..1387096 (-) 2019 WP_132302244.1 NAD-dependent DNA ligase LigA -
  O1Q83_RS06845 (O1Q83_01347) zipA 1387180..1388178 (-) 999 WP_132302243.1 cell division protein ZipA -
  O1Q83_RS06850 (O1Q83_01348) cysZ 1388324..1389175 (+) 852 WP_132302242.1 sulfate transporter CysZ -
  O1Q83_RS06855 (O1Q83_01349) pilF/pilF2 1389194..1389715 (-) 522 WP_132302241.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  O1Q83_RS06860 (O1Q83_01350) - 1389815..1390939 (-) 1125 WP_132302240.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  O1Q83_RS06865 (O1Q83_01351) - 1391267..1392547 (-) 1281 WP_132302239.1 type II toxin-antitoxin system HipA family toxin -
  O1Q83_RS06870 (O1Q83_01352) - 1392547..1392864 (-) 318 WP_228777452.1 transcriptional regulator -
  O1Q83_RS06875 (O1Q83_01353) - 1392962..1393651 (-) 690 WP_132302238.1 c-type cytochrome biogenesis protein -
  O1Q83_RS06880 (O1Q83_01354) - 1393648..1394046 (-) 399 WP_132302237.1 cytochrome c-type biogenesis protein -
  O1Q83_RS06885 (O1Q83_01355) - 1394046..1394591 (-) 546 WP_132302367.1 DsbE family thiol:disulfide interchange protein -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 19785.25 Da        Isoelectric Point: 6.2312

>NTDB_id=1058285 O1Q83_RS06855 WP_132302241.1 1389194..1389715(-) (pilF/pilF2) [Lonepinella koalarum strain ACM3666]
MKFITHFILILFLTACVSQNAEIDFDQKPAAKARVELALGYLSQQDWEPAKQNLDKALSYAPDYYLVHSAFAYFYQQQGN
ITQAREAYLKAIKLDDKQGDVHNNYGTFLCSQGEFQAAYQQFDLAVVSPNYYRQADSYENKALCALSENNQAVYQQSKIA
LEKADPERAKKLR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=1058285 O1Q83_RS06855 WP_132302241.1 1389194..1389715(-) (pilF/pilF2) [Lonepinella koalarum strain ACM3666]
ATGAAATTTATAACGCACTTTATTCTTATTCTTTTTTTAACCGCTTGCGTTTCGCAAAATGCTGAAATAGATTTTGATCA
AAAGCCAGCCGCCAAAGCTCGAGTAGAACTCGCACTGGGCTATTTATCCCAACAAGATTGGGAACCTGCTAAACAAAATC
TGGATAAAGCCTTGAGTTATGCTCCCGATTACTATTTAGTTCATTCCGCCTTTGCTTATTTTTACCAACAACAAGGCAAT
ATTACGCAAGCACGAGAAGCCTATTTGAAAGCCATTAAACTAGATGATAAACAAGGCGATGTGCATAATAATTATGGTAC
ATTTCTTTGTTCGCAAGGGGAGTTCCAAGCTGCTTATCAGCAATTTGATTTAGCGGTGGTAAGCCCGAATTATTATCGTC
AAGCAGATAGTTATGAAAATAAAGCCTTGTGTGCTTTATCGGAAAATAATCAGGCGGTTTATCAACAAAGTAAAATAGCG
TTAGAAAAAGCAGATCCTGAAAGGGCGAAGAAATTAAGATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_19)

(42-98)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4R1KUL6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

53.216

98.844

0.526
  pilF Glaesserella parasuis strain SC1401

45.763

100

0.468

Multiple sequence alignment