Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   ACFMSI_RS07665 Genome accession   NZ_CP170579
Coordinates   1564889..1565959 (+) Length   356 a.a.
NCBI ID   WP_000776425.1    Uniprot ID   -
Organism   Staphylococcus aureus strain JB2E56     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1559889..1570959
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFMSI_RS07635 (ACFMSI_07635) - 1560312..1561775 (+) 1464 WP_001017856.1 rhomboid family intramembrane serine protease -
  ACFMSI_RS07640 (ACFMSI_07640) - 1561756..1561959 (+) 204 WP_000087559.1 YqgQ family protein -
  ACFMSI_RS07645 (ACFMSI_07645) - 1561956..1562942 (+) 987 WP_000161315.1 ROK family glucokinase -
  ACFMSI_RS07650 (ACFMSI_07650) - 1562942..1563271 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ACFMSI_RS07655 (ACFMSI_07655) - 1563268..1563891 (+) 624 WP_113587809.1 MBL fold metallo-hydrolase -
  ACFMSI_RS07660 (ACFMSI_07660) comGA 1563943..1564917 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  ACFMSI_RS07665 (ACFMSI_07665) comGB 1564889..1565959 (+) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACFMSI_RS07670 (ACFMSI_07670) comGC 1565973..1566284 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  ACFMSI_RS07675 (ACFMSI_07675) comGD 1566262..1566708 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACFMSI_RS07680 (ACFMSI_07680) comGE 1566695..1566994 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  ACFMSI_RS07685 (ACFMSI_07685) - 1566912..1567337 (+) 426 Protein_1480 competence type IV pilus minor pilin ComGF -
  ACFMSI_RS07690 (ACFMSI_07690) - 1567433..1568605 (+) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  ACFMSI_RS07695 (ACFMSI_07695) - 1568655..1568741 (+) 87 Protein_1482 type IV pilus minor pilin ComGF family protein -
  ACFMSI_RS07700 (ACFMSI_07700) - 1568838..1568984 (+) 147 WP_001789879.1 hypothetical protein -
  ACFMSI_RS07705 (ACFMSI_07705) - 1568974..1569498 (+) 525 WP_001015120.1 shikimate kinase -
  ACFMSI_RS07710 (ACFMSI_07710) gcvT 1569657..1570748 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41489.97 Da        Isoelectric Point: 10.1030

>NTDB_id=1058132 ACFMSI_RS07665 WP_000776425.1 1564889..1565959(+) (comGB) [Staphylococcus aureus strain JB2E56]
MKLQWINTFKLHSKKRQLSKVQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNKLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1058132 ACFMSI_RS07665 WP_000776425.1 1564889..1565959(+) (comGB) [Staphylococcus aureus strain JB2E56]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGTCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGCACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAACCAGATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATCATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTCTCTATTTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAAATTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CGGGTTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTACCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGAAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.438

100

0.994
  comGB Staphylococcus aureus N315

99.438

100

0.994

Multiple sequence alignment