Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACFJIX_RS08940 Genome accession   NZ_CP170545
Coordinates   1906394..1907917 (-) Length   507 a.a.
NCBI ID   WP_377159227.1    Uniprot ID   -
Organism   Roseateles sp. UC29_93     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1901394..1912917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJIX_RS08905 (ACFJIX_08905) - 1902467..1902709 (+) 243 WP_377159213.1 hypothetical protein -
  ACFJIX_RS08910 (ACFJIX_08910) - 1902763..1903320 (+) 558 WP_377159215.1 NADPH-dependent FMN reductase -
  ACFJIX_RS08915 (ACFJIX_08915) - 1903324..1904067 (+) 744 WP_377159217.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACFJIX_RS08920 (ACFJIX_08920) - 1904088..1904306 (-) 219 WP_377159219.1 hypothetical protein -
  ACFJIX_RS08925 (ACFJIX_08925) - 1904344..1904634 (-) 291 WP_377159221.1 type II toxin-antitoxin system RelE/ParE family toxin -
  ACFJIX_RS08930 (ACFJIX_08930) - 1904634..1904894 (-) 261 WP_377159223.1 type II toxin-antitoxin system ParD family antitoxin -
  ACFJIX_RS08935 (ACFJIX_08935) - 1904963..1906249 (-) 1287 WP_377159225.1 class I SAM-dependent RNA methyltransferase -
  ACFJIX_RS08940 (ACFJIX_08940) rpoN/rpoN1 1906394..1907917 (-) 1524 WP_377159227.1 RNA polymerase factor sigma-54 Machinery gene
  ACFJIX_RS08945 (ACFJIX_08945) lptB 1907933..1908700 (-) 768 WP_377159229.1 LPS export ABC transporter ATP-binding protein -
  ACFJIX_RS08950 (ACFJIX_08950) lptA 1908697..1909320 (-) 624 WP_377159231.1 lipopolysaccharide transport periplasmic protein LptA -
  ACFJIX_RS08955 (ACFJIX_08955) - 1909657..1911351 (-) 1695 WP_377159233.1 ABC transporter ATP-binding protein -
  ACFJIX_RS08960 (ACFJIX_08960) - 1911356..1912408 (-) 1053 WP_377159235.1 ABC transporter permease -

Sequence

Protein


Download         Length: 507 a.a.        Molecular weight: 56530.53 Da        Isoelectric Point: 4.5488

>NTDB_id=1057879 ACFJIX_RS08940 WP_377159227.1 1906394..1907917(-) (rpoN/rpoN1) [Roseateles sp. UC29_93]
MRPTLQVRLSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLASNPLLETDEEFDAPLPTATQERLTEREEREERVAQTES
DAPAGDTPAGDEMRAEEFGTTERDDWENGTERDDFDGIRETPGSASSSSGDDDERPETEAPDESLQDVLRGQIPGMNLSA
VDRAALLALIESLNEDGYLEDPLEELVAAILGEDADLEQREELLDRFKVALKWLQSMEPAGVGARDLGECLELQLRTLPR
SPEQALAIVICRRHLDLLAKRDWRRLMSLTGADEELLRDGQSLIAGLEPKPGRRYARSENRPVIPDVIVQKAGRNFRVSI
NPDVLPKLRINDLYAQALRATRGGVSNSPLGGQLQEARWFIKNIQQRFDTIQRVSEAIVERQRGFFTHGELAMKPLVLRE
IADELGLHESTISRVTTAKYMATPWGTFELKYFFGSSLSTEAGGNASSTAVRALIKQFIAAEDTTSPLSDSQLSTMLGEQ
GITVARRTVAKYREALKLAPANLRKSR

Nucleotide


Download         Length: 1524 bp        

>NTDB_id=1057879 ACFJIX_RS08940 WP_377159227.1 1906394..1907917(-) (rpoN/rpoN1) [Roseateles sp. UC29_93]
ATGAGGCCGACGCTACAGGTTCGCCTGTCCCAGCATCTTGCGCTGACGCCTCAGCTGCAGCAGTCCATCCGTCTGTTGCA
GTTGTCGACGCTGGAGCTCCATCAGGAGGTGGAGCAGATGCTCGCGAGCAACCCGCTGCTGGAGACCGACGAGGAATTCG
ACGCCCCGCTGCCGACCGCCACGCAGGAGCGTCTGACCGAGCGCGAGGAACGCGAGGAGCGCGTCGCGCAGACGGAATCG
GACGCGCCCGCCGGGGACACGCCCGCCGGCGACGAGATGCGTGCCGAGGAGTTCGGCACCACCGAACGCGACGACTGGGA
GAACGGCACCGAGCGTGACGACTTCGACGGCATCCGGGAAACGCCGGGCAGCGCGTCCTCCTCATCCGGGGACGACGACG
AGCGGCCCGAGACCGAAGCGCCCGACGAATCGCTGCAGGACGTGCTGCGCGGACAGATCCCCGGCATGAACCTCAGCGCG
GTGGATCGCGCGGCGCTGCTGGCGCTGATCGAATCGCTCAACGAAGACGGTTATCTCGAGGACCCGCTCGAGGAGCTGGT
CGCCGCGATCCTCGGCGAGGACGCCGATCTGGAGCAGCGCGAGGAGCTGCTGGACCGCTTCAAGGTCGCGCTCAAGTGGC
TGCAGTCGATGGAGCCCGCCGGTGTCGGCGCGCGCGACCTCGGCGAGTGCCTGGAGCTGCAGCTGCGCACGCTGCCGCGC
TCGCCCGAGCAGGCGCTGGCGATCGTGATCTGCCGCCGGCACCTGGACCTGCTCGCCAAGCGCGACTGGCGCCGGCTGAT
GTCGCTGACCGGCGCCGACGAGGAGCTGCTGCGCGACGGGCAATCGCTGATCGCCGGCCTGGAGCCCAAGCCGGGCCGTC
GCTACGCGCGCAGCGAGAACCGGCCCGTCATCCCCGACGTGATCGTGCAGAAGGCGGGGCGCAATTTCCGCGTCTCCATC
AACCCGGACGTGCTGCCCAAGCTGCGCATCAACGATCTCTACGCGCAGGCGCTGCGCGCGACGCGCGGCGGCGTGAGCAA
CTCGCCGCTGGGCGGGCAGCTGCAGGAAGCACGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACGATCCAGCGTG
TCAGCGAGGCGATCGTCGAGCGCCAGCGCGGATTCTTCACGCACGGCGAGCTGGCGATGAAGCCGCTGGTGCTGCGCGAG
ATCGCGGACGAGCTGGGCTTGCATGAATCGACGATCTCTCGCGTCACGACCGCCAAGTACATGGCGACGCCGTGGGGCAC
TTTCGAGCTGAAGTACTTCTTCGGGTCGAGCCTGTCGACCGAGGCCGGCGGCAATGCGTCCAGCACGGCGGTGCGCGCGC
TGATCAAGCAGTTCATCGCGGCCGAGGACACCACCAGCCCGCTGTCCGACAGCCAGCTGTCGACGATGCTCGGCGAGCAG
GGCATCACCGTGGCGCGACGCACCGTCGCGAAGTACCGCGAGGCGCTCAAGCTCGCGCCGGCCAATCTGCGCAAGTCGCG
CTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(353-505)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(140-334)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.157

100

0.525

Multiple sequence alignment