Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ACFIOL_RS05950 Genome accession   NZ_CP170416
Coordinates   1162726..1163412 (+) Length   228 a.a.
NCBI ID   WP_410534690.1    Uniprot ID   -
Organism   Staphylococcus aureus strain t21644     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1157726..1168412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFIOL_RS05915 yqeH 1158253..1159353 (+) 1101 WP_001280141.1 ribosome biogenesis GTPase YqeH -
  ACFIOL_RS05920 aroE 1159367..1160173 (+) 807 WP_000666748.1 shikimate dehydrogenase -
  ACFIOL_RS05925 yhbY 1160177..1160467 (+) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  ACFIOL_RS05930 nadD 1160470..1161039 (+) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  ACFIOL_RS05935 yqeK 1161029..1161613 (+) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ACFIOL_RS05940 rsfS 1161614..1161967 (+) 354 WP_001088022.1 ribosome silencing factor -
  ACFIOL_RS05945 - 1161970..1162686 (+) 717 WP_000084829.1 class I SAM-dependent DNA methyltransferase -
  ACFIOL_RS05950 comEA 1162726..1163412 (+) 687 WP_410534690.1 helix-hairpin-helix domain-containing protein Machinery gene
  ACFIOL_RS05955 - 1163504..1163965 (+) 462 WP_000439693.1 ComE operon protein 2 -
  ACFIOL_RS05960 comEC 1163970..1166171 (+) 2202 WP_410534691.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACFIOL_RS05965 holA 1166228..1167202 (+) 975 WP_001282568.1 DNA polymerase III subunit delta -
  ACFIOL_RS05970 rpsT 1167247..1167498 (-) 252 WP_001274017.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25814.51 Da        Isoelectric Point: 9.6844

>NTDB_id=1057014 ACFIOL_RS05950 WP_410534690.1 1162726..1163412(+) (comEA) [Staphylococcus aureus strain t21644]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDNALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIGVNSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQARANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTI

Nucleotide


Download         Length: 687 bp        

>NTDB_id=1057014 ACFIOL_RS05950 WP_410534690.1 1162726..1163412(+) (comEA) [Staphylococcus aureus strain t21644]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATAATG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGGAGTAAACAGTGTGCATGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACCGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAGAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGATATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

99.561

100

0.996
  comEA Staphylococcus aureus N315

96.93

100

0.969

Multiple sequence alignment