Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   O1Q79_RS05395 Genome accession   NZ_CP170386
Coordinates   1126781..1127302 (-) Length   173 a.a.
NCBI ID   WP_386690655.1    Uniprot ID   -
Organism   Lonepinella sp. MS14434     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1121781..1132302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1Q79_RS05380 (O1Q79_01077) ligA 1122658..1124676 (-) 2019 WP_386690650.1 NAD-dependent DNA ligase LigA -
  O1Q79_RS05385 (O1Q79_01078) zipA 1124760..1125764 (-) 1005 WP_386690652.1 cell division protein ZipA -
  O1Q79_RS05390 (O1Q79_01079) cysZ 1125911..1126762 (+) 852 WP_386687062.1 sulfate transporter CysZ -
  O1Q79_RS05395 (O1Q79_01080) pilF/pilF2 1126781..1127302 (-) 522 WP_386690655.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  O1Q79_RS05400 (O1Q79_01081) - 1127346..1128470 (-) 1125 WP_386690657.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  O1Q79_RS05405 (O1Q79_01082) - 1128702..1129316 (-) 615 Protein_1047 tetratricopeptide repeat protein -
  O1Q79_RS05410 (O1Q79_01083) - 1129388..1129780 (-) 393 WP_386690659.1 cytochrome c-type biogenesis protein CcmH -
  O1Q79_RS05415 (O1Q79_01084) - 1129780..1130325 (-) 546 WP_386693353.1 DsbE family thiol:disulfide interchange protein -
  O1Q79_RS05420 (O1Q79_01085) - 1130325..1132271 (-) 1947 WP_386690662.1 heme lyase CcmF/NrfE family subunit -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 19800.22 Da        Isoelectric Point: 6.2312

>NTDB_id=1056515 O1Q79_RS05395 WP_386690655.1 1126781..1127302(-) (pilF/pilF2) [Lonepinella sp. MS14434]
MKFIPHFILILFLTACVSQNAEIDFDQKPAAKARVELALGYLSQQDWEQAKQNLDKALSYAPDYYLVHSAFAYFYQQQGN
ITQARESYLKAIKLDDKQGDVHNNYGTFLCSQGEFQAAYQQFDLAVASPNYYRQADSYENKALCALSENNQAVYQQSKIA
LEKADPERAKKLR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=1056515 O1Q79_RS05395 WP_386690655.1 1126781..1127302(-) (pilF/pilF2) [Lonepinella sp. MS14434]
ATGAAATTTATACCGCACTTTATACTTATTCTTTTTTTAACTGCTTGCGTTTCGCAAAATGCTGAGATAGATTTTGATCA
AAAGCCAGCCGCCAAAGCTCGAGTAGAACTCGCACTGGGTTATTTATCCCAGCAAGATTGGGAACAAGCTAAACAAAATC
TGGATAAAGCCTTGAGTTATGCCCCTGATTACTATTTAGTTCATTCTGCCTTTGCCTATTTTTACCAACAACAAGGCAAT
ATTACACAAGCCCGTGAATCTTATTTGAAAGCCATTAAACTAGATGATAAACAAGGCGATGTGCATAATAATTATGGTAC
ATTTCTTTGTTCGCAAGGGGAGTTCCAAGCTGCTTATCAGCAATTTGATTTAGCGGTGGCAAGCCCGAATTATTATCGTC
AAGCAGATAGTTATGAAAATAAAGCCTTGTGTGCTTTATCGGAAAATAATCAGGCTGTTTATCAACAAAGTAAAATAGCG
TTAGAAAAAGCAGATCCTGAAAGGGCGAAGAAATTAAGATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_19)

(42-86)

Tetratricopeptide repeat (TPR_17)

(88-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

54.545

95.376

0.52
  pilF Glaesserella parasuis strain SC1401

46.471

98.266

0.457

Multiple sequence alignment