Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   O1Q81_RS08765 Genome accession   NZ_CP170385
Coordinates   1813600..1814121 (-) Length   173 a.a.
NCBI ID   WP_386697139.1    Uniprot ID   -
Organism   Lonepinella sp. MS14436     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1808600..1819121
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1Q81_RS08750 (O1Q81_01744) ligA 1809477..1811495 (-) 2019 WP_386697132.1 NAD-dependent DNA ligase LigA -
  O1Q81_RS08755 (O1Q81_01745) zipA 1811579..1812583 (-) 1005 WP_386697134.1 cell division protein ZipA -
  O1Q81_RS08760 (O1Q81_01746) cysZ 1812730..1813581 (+) 852 WP_386697137.1 sulfate transporter CysZ -
  O1Q81_RS08765 (O1Q81_01747) pilF/pilF2 1813600..1814121 (-) 522 WP_386697139.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  O1Q81_RS08770 (O1Q81_01748) - 1814166..1815302 (-) 1137 WP_386697141.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  O1Q81_RS08775 (O1Q81_01749) raiA 1815533..1815853 (-) 321 WP_386687068.1 ribosome-associated translation inhibitor RaiA -
  O1Q81_RS08780 (O1Q81_01750) metG 1816053..1818131 (-) 2079 WP_386697143.1 methionine--tRNA ligase -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 19929.38 Da        Isoelectric Point: 6.2312

>NTDB_id=1056499 O1Q81_RS08765 WP_386697139.1 1813600..1814121(-) (pilF/pilF2) [Lonepinella sp. MS14436]
MKFIPHFILILFLTACVSQNAEIDFDQKPAAKARVELALGYLSQQDWEQAKQNLDKALSYAPDYYLVHSAFAYFYQQQGN
ITQARESYLKAIKLDDKQGDVHNNYGTFLCSQWEFQAAYQQFDLAVASPNYYRQADSYENKALCALSENNQAVYQQSKIA
LEKADPERAKKLR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=1056499 O1Q81_RS08765 WP_386697139.1 1813600..1814121(-) (pilF/pilF2) [Lonepinella sp. MS14436]
ATGAAATTTATACCGCACTTTATACTTATTCTTTTTTTAACTGCTTGCGTTTCGCAAAATGCTGAAATAGATTTTGATCA
AAAGCCAGCCGCCAAAGCTCGAGTAGAACTCGCACTGGGTTATTTATCCCAGCAAGATTGGGAACAAGCTAAACAAAATC
TGGATAAAGCCTTGAGTTATGCCCCTGATTACTATTTGGTTCATTCCGCCTTTGCTTATTTTTACCAACAACAAGGCAAT
ATTACACAAGCCCGTGAATCTTATTTGAAAGCCATTAAACTAGATGATAAACAAGGCGATGTGCATAATAATTATGGTAC
ATTTCTTTGTTCGCAATGGGAGTTCCAAGCTGCTTATCAGCAATTTGATTTAGCAGTGGCAAGCCCGAATTATTATCGTC
AAGCAGATAGTTATGAAAATAAAGCCTTGTGTGCTTTATCGGAAAATAATCAGGCGGTTTATCAACAAAGTAAAATAGCG
TTAGAAAAAGCAGATCCTGAAAGGGCGAAGAAATTAAGATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_19)

(42-98)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

54.545

95.376

0.52
  pilF Glaesserella parasuis strain SC1401

46.471

98.266

0.457

Multiple sequence alignment