Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AB5G97_RS19900 Genome accession   NZ_CP170371
Coordinates   4226377..4227816 (-) Length   479 a.a.
NCBI ID   WP_005336331.1    Uniprot ID   A0A0T6N7F3
Organism   Aeromonas veronii strain Aer12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4221377..4232816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB5G97_RS19870 (AB5G97_19870) - 4221645..4222385 (+) 741 WP_204380319.1 KDGP aldolase family protein -
  AB5G97_RS19875 (AB5G97_19875) - 4222493..4224397 (+) 1905 WP_376868773.1 BglG family transcription antiterminator -
  AB5G97_RS19880 (AB5G97_19880) npr 4224415..4224687 (-) 273 WP_005336318.1 PTS phosphocarrier protein NPr -
  AB5G97_RS19885 (AB5G97_19885) rapZ 4224708..4225574 (-) 867 WP_005341768.1 RNase adapter RapZ -
  AB5G97_RS19890 (AB5G97_19890) ptsN 4225620..4226066 (-) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AB5G97_RS19895 (AB5G97_19895) hpf 4226069..4226356 (-) 288 WP_005336323.1 ribosome hibernation promoting factor -
  AB5G97_RS19900 (AB5G97_19900) rpoN/rpoN1 4226377..4227816 (-) 1440 WP_005336331.1 RNA polymerase factor sigma-54 Machinery gene
  AB5G97_RS19905 (AB5G97_19905) lptB 4227882..4228607 (-) 726 WP_005336333.1 LPS export ABC transporter ATP-binding protein -
  AB5G97_RS19910 (AB5G97_19910) lptA 4228611..4229138 (-) 528 WP_040068817.1 lipopolysaccharide transport periplasmic protein LptA -
  AB5G97_RS19915 (AB5G97_19915) lptC 4229125..4229685 (-) 561 WP_005336338.1 LPS export ABC transporter periplasmic protein LptC -
  AB5G97_RS19920 (AB5G97_19920) kdsC 4229682..4230236 (-) 555 WP_088868289.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AB5G97_RS19925 (AB5G97_19925) - 4230236..4231231 (-) 996 WP_058057518.1 KpsF/GutQ family sugar-phosphate isomerase -
  AB5G97_RS19930 (AB5G97_19930) mlaF 4231439..4232239 (+) 801 WP_005336343.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53701.64 Da        Isoelectric Point: 4.3442

>NTDB_id=1056376 AB5G97_RS19900 WP_005336331.1 4226377..4227816(-) (rpoN/rpoN1) [Aeromonas veronii strain Aer12]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=1056376 AB5G97_RS19900 WP_005336331.1 4226377..4227816(-) (rpoN/rpoN1) [Aeromonas veronii strain Aer12]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAACCCTCTGCTGGAGCAGGAAGAGAGCGAGG
CGCAAGAGACCAGTCCGGAAGCGCCGGTCGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCCCAGGAGCAGATGCCG
GATGAGTTGCCGGTGGATACCACCTGGGACGAGGTTTACTCTGCCGGCACCGCCCAGAGCGCGGGCCCCATCCACGATGG
GGAAGATAGCGTCTATCAGGGTGAAACTACCGAAAACCTGCAGGATTATCTGCTGTGGCAGATGCGGCTCACACCGTTCA
GCGATGTGGATGCCGCCATCGCGCTCGCCATCATCGATGCCATCGACGAATCCGGTTATCTGACCGTTGCGCTGGAGGAT
ATCCAGGCTGCCGTCACCAGCGATGACGTAGAGGTGGAGCTCGATGAGGTCGAGGCGGTACTCAAGCGGGTTCAGCACTT
CGATCCGGTCGGCATTGCTGCCCGTTCTGTGCAGGAGTGTTTGCTGATCCAGCTGGATCAGTATGATCCGCAGACCCCCT
GGCTCAACGAGGCCAAGGAGGTGATCCTCGAGCACATGGAGCTGCTCGGCAACCGGGATTACCGCACTCTCGCCCGCAAG
ACAAAACTCAAAGAGAGCGACCTCAAGGAGGTGCTGGATCTGATCCAGCTGCTGGAGCCGCGTCCCGGCAATGGCATTAT
CCAGAGCGACTCTCAGTACGTGATCCCCGACGTCTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCAGCCCCCCGCATCCGGGTCAATGAGACCTATGCCGCCATGGCCCGCAACGCCCGCAGCAGCACCGACACCCAGTTT
ATCCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCTTGGAAAGTCGCAATGACACCCTACTGAAAGTGGCCAG
TTGCATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCGATGGTGCTCAATGACATCGCCG
AAGCGGTAGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCCCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6N7F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491

Multiple sequence alignment