Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   ACFDA5_RS00220 Genome accession   NZ_CP170246
Coordinates   45556..46905 (-) Length   449 a.a.
NCBI ID   WP_381411616.1    Uniprot ID   -
Organism   Staphylococcus epidermidis strain Q8     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 43927..78315 45556..46905 within 0
IScluster/Tn 47232..61227 45556..46905 flank 327


Gene organization within MGE regions


Location: 43927..78315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFDA5_RS00220 (ACFDA5_00220) ccrA 45556..46905 (-) 1350 WP_381411616.1 cassette chromosome recombinase CcrA Machinery gene
  ACFDA5_RS00225 (ACFDA5_00225) - 47232..48909 (+) 1678 Protein_42 IS1182 family transposase -
  ACFDA5_RS00230 (ACFDA5_00230) cch1 49052..50845 (-) 1794 WP_064212311.1 cassette chromosome replicative helicase -
  ACFDA5_RS00235 (ACFDA5_00235) ssb 50845..51138 (-) 294 WP_381411617.1 cassette chromosome ssDNA-binding protein -
  ACFDA5_RS00240 (ACFDA5_00240) - 51373..52514 (+) 1142 WP_381411619.1 IS3 family transposase -
  ACFDA5_RS00245 (ACFDA5_00245) - 52585..54099 (-) 1515 WP_381411621.1 hypothetical protein -
  ACFDA5_RS00250 (ACFDA5_00250) - 54730..55641 (+) 912 WP_046464619.1 ABC transporter substrate-binding protein -
  ACFDA5_RS00255 (ACFDA5_00255) - 55891..56565 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  ACFDA5_RS00260 (ACFDA5_00260) - 56601..56990 (+) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  ACFDA5_RS00265 (ACFDA5_00265) - 57907..58650 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  ACFDA5_RS00270 (ACFDA5_00270) - 58747..59175 (+) 429 WP_000872606.1 MaoC family dehydratase -
  ACFDA5_RS00275 (ACFDA5_00275) mecA 59221..61230 (-) 2010 WP_103298748.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  ACFDA5_RS00280 (ACFDA5_00280) mecR1 61327..63084 (+) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  ACFDA5_RS00285 (ACFDA5_00285) mecI 63084..63455 (+) 372 WP_000369214.1 mecA-type methicillin resistance repressor MecI -
  ACFDA5_RS00290 (ACFDA5_00290) psm-mec 63540..63608 (-) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  ACFDA5_RS00295 (ACFDA5_00295) - 63928..65076 (+) 1149 WP_000427807.1 ROK family transcriptional regulator -
  ACFDA5_RS00300 (ACFDA5_00300) cstB 65190..66524 (-) 1335 Protein_57 persulfide dioxygenase-sulfurtransferase CstB -
  ACFDA5_RS00305 (ACFDA5_00305) - 66557..67624 (-) 1068 WP_115359094.1 DsrE/DsrF/DrsH-like family protein -
  ACFDA5_RS00310 (ACFDA5_00310) cstR 67720..67980 (+) 261 WP_049382303.1 persulfide-sensing transcriptional repressor CstR -
  ACFDA5_RS00315 (ACFDA5_00315) - 67977..68735 (+) 759 WP_000623679.1 sulfite exporter TauE/SafE family protein -
  ACFDA5_RS00320 (ACFDA5_00320) - 68905..69102 (+) 198 Protein_61 heavy metal translocating P-type ATPase -
  ACFDA5_RS00325 (ACFDA5_00325) - 69120..69665 (+) 546 WP_000592151.1 YdhK family protein -
  ACFDA5_RS00330 (ACFDA5_00330) - 70134..70439 (+) 306 WP_000677987.1 hypothetical protein -
  ACFDA5_RS00335 (ACFDA5_00335) - 70637..71500 (+) 864 WP_000680579.1 hypothetical protein -
  ACFDA5_RS00340 (ACFDA5_00340) - 71608..73083 (+) 1476 WP_001138664.1 DUF6038 family protein -
  ACFDA5_RS00345 (ACFDA5_00345) - 73310..74410 (+) 1101 WP_000449985.1 DNA polymerase -
  ACFDA5_RS00350 (ACFDA5_00350) - 74403..74774 (+) 372 WP_001058157.1 hypothetical protein -
  ACFDA5_RS00355 (ACFDA5_00355) - 74771..76414 (+) 1644 WP_001015579.1 DUF5906 domain-containing protein -
  ACFDA5_RS00360 (ACFDA5_00360) ccrC 76639..78315 (+) 1677 WP_012086571.1 cassette chromosome recombinase CcrC -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52577.28 Da        Isoelectric Point: 9.9714

>NTDB_id=1055889 ACFDA5_RS00220 WP_381411616.1 45556..46905(-) (ccrA) [Staphylococcus epidermidis strain Q8]
MKKVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQMTQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYRVHILSVHDGYFDMDQAFDRFKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRLQKQTKQTSSDNQLKQKINCPCCNATLTNMTVRKKNHILRYYVCPKNMNASRFVCDFKGINAQTLEDKVLKV
CQDFYQDHHIYAKIKNAIDKRLKRQRNTETHHTLTQEKLIEKLAQGIIDAETFRKQMQSLHQQPQRITSISEHQIQHTIQ
NIIQKRFTLNILSPYIETIHIAKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=1055889 ACFDA5_RS00220 WP_381411616.1 45556..46905(-) (ccrA) [Staphylococcus epidermidis strain Q8]
ATGAAAAAAGTTATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATGACACAATTAATTCAACAAGGACAATGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
TTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACATATCGCGTTCATATCTTAAGTGTGCATGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGATTCAAGCTTAATATTTTTATCAGCTTGGCCGAACTTGAATCAGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACCCATGCGCCCTTTGGTTACGAATATCAC
AACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATTGCAAAAACAAACCAAACAGACATCTTCGGATAATCAACTCAAACAAAAAATCAA
TTGCCCATGTTGTAATGCAACACTTACAAATATGACCGTTAGAAAAAAGAATCATATATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTCAAAGGCATCAATGCACAGACATTGGAAGATAAAGTATTAAAAGTG
TGCCAAGACTTTTATCAAGACCACCATATCTACGCAAAAATTAAAAATGCGATTGACAAACGCTTAAAAAGACAACGAAA
CACAGAAACACATCACACACTGACTCAAGAAAAACTAATAGAAAAGTTAGCCCAAGGCATCATCGATGCAGAAACGTTCA
GAAAACAAATGCAATCATTACATCAACAACCGCAACGCATCACATCCATCAGTGAGCATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTGAACATATTGTCCCCCTATATTGAAACCATTCACATTGCGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATCGTCAATCAAACCATGCAATCATCAATTGCATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)

Recombinase (Recombinase)

(169-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

93.987

100

0.94
  ccrA Staphylococcus aureus COL

73.497

100

0.735

Multiple sequence alignment