Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACFDAU_RS13675 Genome accession   NZ_CP170197
Coordinates   2756027..2757466 (+) Length   479 a.a.
NCBI ID   WP_413437448.1    Uniprot ID   -
Organism   Sulfuriferula sp. GW1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2751027..2762466
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFDAU_RS13650 (ACFDAU_13650) - 2752532..2753527 (+) 996 WP_413437443.1 KpsF/GutQ family sugar-phosphate isomerase -
  ACFDAU_RS13655 (ACFDAU_13655) - 2753527..2754066 (+) 540 WP_413437444.1 KdsC family phosphatase -
  ACFDAU_RS13660 (ACFDAU_13660) lptC 2754063..2754635 (+) 573 WP_413437445.1 LPS export ABC transporter periplasmic protein LptC -
  ACFDAU_RS13665 (ACFDAU_13665) lptA 2754619..2755224 (+) 606 WP_413437446.1 lipopolysaccharide transport periplasmic protein LptA -
  ACFDAU_RS13670 (ACFDAU_13670) lptB 2755221..2755943 (+) 723 WP_413437447.1 LPS export ABC transporter ATP-binding protein -
  ACFDAU_RS13675 (ACFDAU_13675) rpoN/rpoN1 2756027..2757466 (+) 1440 WP_413437448.1 RNA polymerase factor sigma-54 Machinery gene
  ACFDAU_RS13680 (ACFDAU_13680) hpf 2757485..2757820 (+) 336 WP_413437449.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACFDAU_RS13685 (ACFDAU_13685) ptsN 2757830..2758285 (+) 456 WP_413437450.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACFDAU_RS13690 (ACFDAU_13690) hprK 2758288..2759229 (+) 942 WP_413439551.1 HPr(Ser) kinase/phosphatase -
  ACFDAU_RS13695 (ACFDAU_13695) rapZ 2759229..2760062 (+) 834 WP_413439552.1 RNase adapter RapZ -
  ACFDAU_RS13700 (ACFDAU_13700) - 2760059..2760433 (+) 375 WP_413437451.1 NusG domain II-containing protein -
  ACFDAU_RS13705 (ACFDAU_13705) - 2760414..2760956 (+) 543 WP_413437452.1 Gx transporter family protein -
  ACFDAU_RS13710 (ACFDAU_13710) - 2760953..2761579 (+) 627 WP_413437453.1 flavin prenyltransferase UbiX -
  ACFDAU_RS13715 (ACFDAU_13715) - 2761627..2762217 (+) 591 WP_413437454.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 54158.28 Da        Isoelectric Point: 4.9107

>NTDB_id=1055509 ACFDAU_RS13675 WP_413437448.1 2756027..2757466(+) (rpoN/rpoN1) [Sulfuriferula sp. GW1]
MKHSLQLKLSQHLTLTPQLQQSIRLLQLSTLELNQELEQMLQDNPLLERVDNLDDGETHINGSDPELRSEAPTETSSVAD
NTPEPETRELGDMDWESEGNYGSPDDDDEDRDYQQPSFETTSLRDHLSWQLNLTPLSERDKRLVLLLIDALDDGGYLTQS
LQDILEMLPPELEVELDDLQIALKNLQNLEPTGIGARNLSECLTLQLLALPEDTPHRDCALTIASTHLHELGSRDYSGLK
RKLHCDDDELRVARDLITHLNPKPGACFGESDTRYIVPDVIVKKVKGLWVASLNPDAMPKLRINRMYADILSRNRDSSHQ
HLASQMQEAKWLIKNVMQRFDTILKVSQSIIDRQRHFFEHGEVAMRPLVLREIAEVVELHESTISRVTTQKFMMTPRGIY
ELKYFFGSHVSTETGGACSATAIRALIKQLVAAENSKKPLSDGQIADLLGQQGIVVARRTIAKYRESLQISPASQRKSL

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=1055509 ACFDAU_RS13675 WP_413437448.1 2756027..2757466(+) (rpoN/rpoN1) [Sulfuriferula sp. GW1]
ATGAAACACAGTCTACAACTCAAGCTATCACAGCATCTCACGTTGACGCCACAGTTGCAGCAGTCTATCCGTCTGTTGCA
ATTGTCCACGCTTGAGCTGAACCAGGAACTGGAGCAAATGCTCCAGGACAACCCATTGCTGGAGCGCGTGGACAATCTGG
ATGACGGTGAAACCCATATCAACGGTAGCGATCCCGAGCTGCGCAGCGAAGCACCTACCGAAACCAGCAGCGTGGCCGAC
AATACTCCGGAGCCGGAAACACGCGAACTAGGCGACATGGACTGGGAAAGCGAGGGCAATTACGGATCGCCCGATGATGA
CGACGAAGACCGCGATTATCAGCAACCTTCTTTCGAGACTACTTCGCTGCGCGATCATCTGTCCTGGCAGCTGAATCTCA
CCCCCTTGTCGGAGCGCGACAAACGCCTGGTGCTGCTGTTGATCGATGCGTTGGACGATGGCGGTTATCTGACCCAAAGC
CTGCAGGACATTCTCGAAATGCTGCCGCCCGAACTGGAAGTCGAGCTCGACGACCTGCAGATCGCACTTAAAAATTTACA
AAACCTCGAGCCCACCGGCATCGGCGCACGCAACCTGTCGGAATGCCTGACTCTGCAATTGCTGGCGCTGCCGGAGGATA
CGCCGCATCGCGATTGTGCGCTGACCATAGCCAGCACGCATTTGCACGAGCTGGGATCGCGCGACTATAGCGGGCTGAAA
CGCAAACTGCATTGCGATGATGATGAGCTACGCGTGGCACGCGATCTGATTACCCATCTCAACCCCAAACCGGGGGCATG
CTTTGGCGAATCGGATACGCGTTACATCGTTCCGGATGTCATTGTAAAAAAAGTCAAAGGTCTGTGGGTGGCGAGCCTCA
ACCCCGACGCCATGCCCAAACTGCGCATCAACCGCATGTACGCCGACATTCTGTCGCGCAACCGCGATAGCAGCCATCAG
CACCTGGCCAGCCAGATGCAGGAAGCCAAATGGCTGATCAAGAACGTCATGCAACGTTTCGATACCATCCTTAAAGTATC
GCAAAGCATTATTGACCGCCAGCGCCATTTTTTCGAGCATGGCGAAGTGGCCATGCGCCCTCTGGTGCTACGCGAGATTG
CCGAGGTCGTGGAACTGCATGAATCCACCATTTCACGCGTCACCACGCAAAAATTCATGATGACGCCGCGTGGCATTTAT
GAACTTAAATACTTTTTCGGCAGTCATGTTAGTACCGAGACCGGGGGTGCCTGTTCTGCCACCGCCATCCGCGCGCTGAT
CAAACAACTGGTCGCCGCTGAAAACAGCAAGAAACCCCTATCCGACGGACAGATTGCAGACCTGCTCGGTCAGCAAGGTA
TCGTGGTGGCACGCCGCACCATTGCCAAATACCGGGAGTCCCTGCAAATATCGCCCGCCAGTCAGCGCAAGTCGCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(117-307)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(321-477)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

56.088

100

0.587

Multiple sequence alignment