Detailed information NTG1052544

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACETK2_RS14375
• Genome accession: NZ_CP169640
• Coordinates: 3224694..3225539 (-)
• Length: 281 a.a.
• NCBI ID: WP_015841038.1
• Uniprot ID: C6DAP4
• Organism: Pectobacterium aroidearum strain Pc3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3219694..3230539

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACETK2_RS14350	-	3219889..3220659 (-)	771	WP_015841034.1	ABC transporter ATP-binding protein	-
ACETK2_RS14355	-	3220661..3221698 (-)	1038	WP_015841035.1	iron ABC transporter permease	-
ACETK2_RS14360	-	3221695..3222759 (-)	1065	WP_015841036.1	ABC transporter substrate-binding protein	-
ACETK2_RS14365	-	3223095..3224120 (+)	1026	WP_015841037.1	LLM class flavin-dependent oxidoreductase	-
ACETK2_RS14370	ypdK	3224477..3224551 (+)	75	WP_099606229.1	membrane protein YpdK	-
ACETK2_RS14375	pilD	3224694..3225539 (-)	846	WP_015841038.1	A24 family peptidase	Machinery gene
ACETK2_RS14380	-	3225647..3226393 (-)	747	WP_015841039.1	type II secretion system protein N	-
ACETK2_RS14385	gspM	3226383..3226877 (-)	495	WP_015841040.1	type II secretion system protein GspM	-
ACETK2_RS14390	gspL	3226874..3228151 (-)	1278	WP_015841041.1	type II secretion system protein GspL	-
ACETK2_RS14395	gspK	3228148..3229134 (-)	987	WP_015841042.1	type II secretion system minor pseudopilin GspK	-
ACETK2_RS14400	gspJ	3229131..3229781 (-)	651	WP_015841043.1	type II secretion system minor pseudopilin GspJ	-
ACETK2_RS14405	gspI	3229781..3230146 (-)	366	WP_015841044.1	type II secretion system minor pseudopilin GspI	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31307.46 Da
• Isoelectric Point: 7.1428

>NTDB_id=1052544 ACETK2_RS14375 WP_015841038.1 3224694..3225539(-) (pilD) [Pectobacterium aroidearum strain Pc3]
MDDLREFALVFPAWWYGALGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELQTGVTDVEHVTSYNLCWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQPVSVQYPLVEVITMLAFLVAGLLWLPGMALCGALILLSFLVVLTVIDIKTLLLPDVL
TLSLLWMGLLFNLSETFVPLRDAVVGAMAGYLSLWLLYWVFKYATGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLIWRGVRKEDTVKPLAFGPWLAIGGVFGVIMNGLYL

Nucleotide

• Length: 846 bp

>NTDB_id=1052544 ACETK2_RS14375 WP_015841038.1 3224694..3225539(-) (pilD) [Pectobacterium aroidearum strain Pc3]
GTGGACGATTTAAGGGAATTCGCACTGGTATTTCCGGCATGGTGGTATGGTGCGCTGGGCGTATTGGGCTTGATTGTTGG
CAGTTTTCTGAACGTCGTGATTTACCGCCTGCCGATTATGTTAGAACGCCGCTGGCGGCAGGACATCGAACTGCAAACGG
GTGTGACCGATGTCGAGCATGTGACGAGTTACAACCTGTGTTGGCCGCCTTCGTCTTGCCCGCACTGTCAGCAGGCGATT
GCGGTGAAAGATAACATCCCGTTATTCAGCTGGCTGTGGCTGCGCGGCCGCTCCCGCTGCTGTCATCAGCCAGTGTCTGT
GCAGTATCCGCTGGTGGAAGTCATTACGATGCTGGCTTTTCTGGTCGCAGGGTTACTGTGGTTGCCCGGCATGGCGTTAT
GCGGGGCGCTGATTCTGCTGTCCTTTCTGGTGGTTTTGACCGTCATTGATATAAAAACGTTACTGCTGCCAGATGTGCTT
ACGCTTTCGCTCTTGTGGATGGGACTGCTGTTTAATCTGTCGGAGACCTTTGTCCCATTACGCGATGCCGTAGTAGGCGC
GATGGCGGGCTATTTGTCCCTGTGGCTACTTTATTGGGTATTTAAATATGCCACAGGCAAAGAAGCGCTAGGGTATGGTG
ATTTTAAGTTACTCGCCGCGCTGGGTGCCTGGCTGGGCTGGCAGGCATTGCCGAATCTGGTCTTGGTGGCGGCGCTGAGC
GGGCTGGTCGTTACTCTCATCTGGCGCGGGGTGCGTAAAGAAGATACGGTTAAACCGTTGGCCTTTGGCCCTTGGCTGGC
GATTGGCGGCGTGTTCGGTGTGATAATGAACGGACTCTATCTGTAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

3D structure

• AlphaFold DB: C6DAP4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.741	96.085	0.488
pilD	Neisseria gonorrhoeae MS11	49.805	91.459	0.456
pilD	Vibrio campbellii strain DS40M4	46.85	90.391	0.423
pilD	Acinetobacter baumannii D1279779	42.336	97.509	0.413
pilD	Acinetobacter nosocomialis M2	42.336	97.509	0.413