Detailed information NTG1052083

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACE17W_RS00145
• Genome accession: NZ_CP169562
• Coordinates: 35275..36138 (+)
• Length: 287 a.a.
• NCBI ID: WP_015444491.1
• Uniprot ID: I7HPT6
• Organism: Legionella pneumophila strain Lp02
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 30275..41138

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACE17W_RS00125 (ACE17W_00125)	-	30768..31094 (-)	327	WP_010947249.1	DUF1820 family protein	-
ACE17W_RS00130 (ACE17W_00130)	-	31277..32020 (-)	744	WP_010947250.1	methyltransferase domain-containing protein	-
ACE17W_RS00135 (ACE17W_00135)	pilB	32279..34003 (+)	1725	WP_010947251.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACE17W_RS00140 (ACE17W_00140)	pilC	34009..35229 (+)	1221	WP_010947252.1	type II secretion system F family protein	Machinery gene
ACE17W_RS00145 (ACE17W_00145)	pilD	35275..36138 (+)	864	WP_015444491.1	prepilin peptidase	Machinery gene
ACE17W_RS00150 (ACE17W_00150)	-	36152..37357 (-)	1206	WP_015444490.1	cyclic nucleotide-binding domain-containing protein	-
ACE17W_RS00155 (ACE17W_00155)	-	37465..38058 (-)	594	WP_010947255.1	hypothetical protein	-
ACE17W_RS00160 (ACE17W_00160)	-	38074..38529 (-)	456	WP_010947256.1	hypothetical protein	-
ACE17W_RS00165 (ACE17W_00165)	-	38663..39478 (+)	816	WP_010947257.1	pyruvate, water dikinase regulatory protein	-
ACE17W_RS00170 (ACE17W_00170)	-	39603..41051 (-)	1449	WP_015444489.1	bifunctional 2-methylcitrate dehydratase/aconitate hydratase	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 32769.36 Da
• Isoelectric Point: 7.8442

>NTDB_id=1052083 ACE17W_RS00145 WP_015444491.1 35275..36138(+) (pilD) [Legionella pneumophila strain Lp02]
MINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFHLEQRKEKIKLNLFLPRSFCPHCKAMVKA
WQNIPLLSYILLRGRCYQCDSPISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTL
GLLWIGLIANTQNVFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITGA
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDSIINWYIGYWM

Nucleotide

• Length: 864 bp

>NTDB_id=1052083 ACE17W_RS00145 WP_015444491.1 35275..36138(+) (pilD) [Legionella pneumophila strain Lp02]
ATGATAAATGCACTAATAATCAATTATCCATGGTTTATGTATTTAGTCGTTGGACTCTTTTCTCTAGCCGTTGGAAGTTT
GCTTAATGTGATAATCTATCGCTTACCAATTATTTTGCAGGAAGAGTGGAAAGAGCAATGCTGTGAGCTATTCCATCTTG
AACAGCGAAAAGAAAAAATAAAACTTAATTTATTTTTACCACGTTCTTTTTGTCCTCATTGTAAAGCGATGGTTAAAGCG
TGGCAGAATATTCCTCTGTTAAGCTATATTTTGTTAAGAGGACGCTGTTACCAATGTGATAGTCCAATTTCAATCCGCTA
CCCTTTTGTTGAGACGTTGACCACAGTTTTATCCTTATACGCCAGTTGGCATTTTGGGTTTACAATACAATTATTATTTG
CTTTATTAGCTATCTGGATATTAATATCACTTGTTTTTATTGATCTTGATCATCAATTACTCCCTGATAGCCTCACTTTA
GGGCTCTTATGGATTGGCCTTATTGCTAATACCCAGAATGTATTTGTTAGTCTTGACGTTGCTGTATTAAGCTGCGCTGG
CGCTTATTTAGCCTTATGGCTATTCATTAATCTGTTTTATCTGATGACAGGTAAAGTAGGTATGGGACATGGAGATTTCA
AATTATTTGCAGCATTTGGTGCCTGGCTTGGATGGATGTATTTACCATTAATTCTACTAATATCTTCAATAACGGGGGCT
ATCATTGGTTTGATTTATTTAAAGATAAATGGTAAATCAAGAGATACTGCAATACCATTTGGCCCTTTTCTTTGCATTTC
TGGATTAATAGCTATGTTTTGGGGAGACTCAATCATCAATTGGTATATAGGTTATTGGATGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-125)

• Type IV leader peptidase family (Peptidase_A24) (136-244)

3D structure

• AlphaFold DB: I7HPT6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.11	97.909	0.481
pilD	Acinetobacter nosocomialis M2	47.985	95.122	0.456
pilD	Acinetobacter baumannii D1279779	47.273	95.819	0.453
pilD	Vibrio campbellii strain DS40M4	45.324	96.864	0.439
pilD	Neisseria gonorrhoeae MS11	42.806	96.864	0.415